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| Title | Cryo-EM Analysis of the Effect of Seeding with Brain-derived Aβ Amyloid Fibrils. |
|---|---|
| Journal, issue, pages | J Mol Biol, Vol. 436, Issue 4, Page 168422, Year 2024 |
| Publish date | Feb 15, 2024 |
 Authors | Peter Benedikt Pfeiffer / Marijana Ugrina / Nadine Schwierz / Christina J Sigurdson / Matthias Schmidt / Marcus Fändrich /   |
| PubMed Abstract | Aβ amyloid fibrils from Alzheimer's brain tissue are polymorphic and structurally different from typical in vitro formed Aβ fibrils. Here, we show that brain-derived (ex vivo) fibril structures can ...Aβ amyloid fibrils from Alzheimer's brain tissue are polymorphic and structurally different from typical in vitro formed Aβ fibrils. Here, we show that brain-derived (ex vivo) fibril structures can be proliferated by seeding in vitro. The proliferation reaction is only efficient for one of the three abundant ex vivo Aβ fibril morphologies, which consists of two peptide stacks, while the inefficiently proliferated fibril morphologies contain four or six peptide stacks. In addition to the seeded fibril structures, we find that de novo nucleated fibril structures can emerge in seeded samples if the seeding reaction is continued over multiple generations. These data imply a competition between de novo nucleation and seed extension and suggest further that seeding favours the outgrowth of fibril morphologies that contain fewer peptide stacks. |
 External links | J Mol Biol / PubMed:38158175 |
| Methods | EM (helical sym.) |
| Resolution | 2.59 - 2.97 Å |
| Structure data | EMDB-17166, PDB-8ot1: EMDB-17167, PDB-8ot3: EMDB-17168, PDB-8ot4: |
| Source |
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 Keywords | PROTEIN FIBRIL /  Amyloid fibril / Amyloid-beta |
