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TitleAssociation of 2-oxoacid dehydrogenase complexes with respirasomes in mitochondria.
Journal, issue, pagesFEBS J, Vol. 291, Issue 1, Page 132-141, Year 2024
Publish dateOct 15, 2023
AuthorsKonstantin S Plokhikh / Semen V Nesterov / Yuriy M Chesnokov / Anton G Rogov / Roman A Kamyshinsky / Aleksandr L Vasiliev / Lev S Yaguzhinsky / Raif G Vasilov /
PubMed AbstractIn the present study, cryo-electron tomography was used to investigate the localization of 2-oxoacid dehydrogenase complexes (OADCs) in cardiac mitochondria and mitochondrial inner membrane samples. ...In the present study, cryo-electron tomography was used to investigate the localization of 2-oxoacid dehydrogenase complexes (OADCs) in cardiac mitochondria and mitochondrial inner membrane samples. Two classes of ordered OADC inner cores with different symmetries were distinguished and their quaternary structures modeled. One class corresponds to pyruvate dehydrogenase complexes and the other to dehydrogenase complexes of α-ketoglutarate and branched-chain α-ketoacids. OADCs were shown to be localized in close proximity to membrane-embedded respirasomes, as observed both in densely packed lamellar cristae of cardiac mitochondria and in ruptured mitochondrial samples where the dense packing is absent. This suggests the specificity of the OADC-respirasome interaction, which allows localized NADH/NAD exchange between OADCs and complex I of the respiratory chain. The importance of this local coupling is based on OADCs being the link between respiration, glycolysis and amino acid metabolism. The coupling of these basic metabolic processes can vary in different tissues and conditions and may be involved in the development of various pathologies. The present study shows that this important and previously missing parameter of mitochondrial complex coupling can be successfully assessed using cryo-electron tomography.
External linksFEBS J / PubMed:37789611
MethodsEM (subtomogram averaging)
Resolution17.0 - 32.0 Å
Structure data

EMDB-16672: Pyruvate dehydrogenase complex core (E2, dihydrolipoyl transacetylase)
Method: EM (subtomogram averaging) / Resolution: 17.0 Å

EMDB-16731: Alpha-ketoglutarate dehydrogenase complex core (E2, dihydrolipoyl transsuccinylase), Branched-chain alpha-ketoacid dehydrogenase complex core (E2, dihydrolipoyl transacylase), indistinguishable
Method: EM (subtomogram averaging) / Resolution: 32.0 Å

Source
  • Rattus norvegicus (Norway rat)

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