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TitleStructural basis for RNA polymerase II ubiquitylation and inactivation in transcription-coupled repair.
Journal, issue, pagesNat Struct Mol Biol, Vol. 31, Issue 3, Page 536-547, Year 2024
Publish dateFeb 5, 2024
AuthorsGoran Kokic / George Yakoub / Diana van den Heuvel / Annelotte P Wondergem / Paula J van der Meer / Yana van der Weegen / Aleksandar Chernev / Isaac Fianu / Thornton J Fokkens / Sonja Lorenz / Henning Urlaub / Patrick Cramer / Martijn S Luijsterburg /
PubMed AbstractDuring transcription-coupled DNA repair (TCR), RNA polymerase II (Pol II) transitions from a transcriptionally active state to an arrested state that allows for removal of DNA lesions. This ...During transcription-coupled DNA repair (TCR), RNA polymerase II (Pol II) transitions from a transcriptionally active state to an arrested state that allows for removal of DNA lesions. This transition requires site-specific ubiquitylation of Pol II by the CRL4 ubiquitin ligase, a process that is facilitated by ELOF1 in an unknown way. Using cryogenic electron microscopy, biochemical assays and cell biology approaches, we found that ELOF1 serves as an adaptor to stably position UVSSA and CRL4 on arrested Pol II, leading to ligase neddylation and activation of Pol II ubiquitylation. In the presence of ELOF1, a transcription factor IIS (TFIIS)-like element in UVSSA gets ordered and extends through the Pol II pore, thus preventing reactivation of Pol II by TFIIS. Our results provide the structural basis for Pol II ubiquitylation and inactivation in TCR.
External linksNat Struct Mol Biol / PubMed:38316879 / PubMed Central
MethodsEM (single particle)
Resolution4.4 Å
Structure data

15827

8b3g

EMDB-15827, PDB-8b3g:
C(N)RL4CSA-UVSSA-E2-ubiquitin complex.
Method: EM (single particle) / Resolution: 4.4 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • homo sapiens (human)
KeywordsTRANSCRIPTION / DNA repair / ubiquitin / cryo-EM

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