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Structure paper

TitleBacterial crystalline cellulose secretion via a supramolecular BcsHD scaffold.
Journal, issue, pagesSci Adv, Vol. 8, Issue 50, Page eadd1170, Year 2022
Publish dateDec 16, 2022
AuthorsWiem Abidi / Marion Decossas / Lucía Torres-Sánchez / Lucie Puygrenier / Sylvie Létoffé / Jean-Marc Ghigo / Petya V Krasteva /
PubMed AbstractCellulose, the most abundant biopolymer on Earth, is not only the predominant constituent of plants but also a key extracellular polysaccharide in the biofilms of many bacterial species. Depending on ...Cellulose, the most abundant biopolymer on Earth, is not only the predominant constituent of plants but also a key extracellular polysaccharide in the biofilms of many bacterial species. Depending on the producers, chemical modifications, and three-dimensional assemblies, bacterial cellulose (BC) can present diverse degrees of crystallinity. Highly ordered, or crystalline, cellulose presents great economical relevance due to its ever-growing number of biotechnological applications. Even if some acetic acid bacteria have long been identified as BC superproducers, the molecular mechanisms determining the secretion of crystalline versus amorphous cellulose remain largely unknown. Here, we present structural and mechanistic insights into the role of the accessory subunits BcsH (CcpAx) and BcsD (CesD) that determine crystalline BC secretion in the lineage. We show that oligomeric BcsH drives the assembly of BcsD into a supramolecular cytoskeletal scaffold that likely stabilizes the cellulose-extruding synthase nanoarrays through an unexpected inside-out mechanism for secretion system assembly.
External linksSci Adv / PubMed:36525496 / PubMed Central
MethodsEM (single particle)
Resolution2.6 - 3.3 Å
Structure data

15039

7zzq

EMDB-15039, PDB-7zzq:
BcsH-BcsD 'beads-on-a-string' filament, local refine
Method: EM (single particle) / Resolution: 2.6 Å

EMDB-15040: BcsHD cis-filaments: four 'beads-on-a-string'
Method: EM (single particle) / Resolution: 2.77 Å

15041

7zzy

EMDB-15041, PDB-7zzy:
Solution BcsD structure
Method: EM (single particle) / Resolution: 3.3 Å

Chemicals

ChemComp-HOH:
WATER

Source
  • komagataeibacter hansenii atcc 23769 (bacteria)
KeywordsSTRUCTURAL PROTEIN / Bacterial cytoskeleton / cellulose secretion / Crystalline cellulose secretion

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