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Title | Structure of the membrane-bound formate hydrogenlyase complex from Escherichia coli. |
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Journal, issue, pages | Nat Commun, Vol. 13, Issue 1, Page 5395, Year 2022 |
Publish date | Sep 14, 2022 |
![]() | Ralf Steinhilper / Gabriele Höff / Johann Heider / Bonnie J Murphy / ![]() |
PubMed Abstract | The prototypical hydrogen-producing enzyme, the membrane-bound formate hydrogenlyase (FHL) complex from Escherichia coli, links formate oxidation at a molybdopterin-containing formate dehydrogenase ...The prototypical hydrogen-producing enzyme, the membrane-bound formate hydrogenlyase (FHL) complex from Escherichia coli, links formate oxidation at a molybdopterin-containing formate dehydrogenase to proton reduction at a [NiFe] hydrogenase. It is of intense interest due to its ability to efficiently produce H during fermentation, its reversibility, allowing H-dependent CO reduction, and its evolutionary link to respiratory complex I. FHL has been studied for over a century, but its atomic structure remains unknown. Here we report cryo-EM structures of FHL in its aerobically and anaerobically isolated forms at resolutions reaching 2.6 Å. This includes well-resolved density for conserved loops linking the soluble and membrane arms believed to be essential in coupling enzymatic turnover to ion translocation across the membrane in the complex I superfamily. We evaluate possible structural determinants of the bias toward hydrogen production over its oxidation and describe an unpredicted metal-binding site near the interface of FdhF and HycF subunits that may play a role in redox-dependent regulation of FdhF interaction with the complex. |
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Methods | EM (single particle) |
Resolution | 2.6 - 3.4 Å |
Structure data | EMDB-14429, PDB-7z0s: EMDB-14430, PDB-7z0t: ![]() EMDB-14431: Structure of the Escherichia coli formate hydrogenlyase complex (aerobic preparation, consensus refinement) ![]() EMDB-14432: Structure of the Escherichia coli formate hydrogenlyase complex (aerobic preparation, focused refinement FdhF) ![]() EMDB-14433: Structure of the Escherichia coli formate hydrogenlyase complex (aerobic preparation, focused refinement peripheral arm) ![]() EMDB-14434: Structure of the Escherichia coli formate hydrogenlyase complex (aerobic preparation, focused refinement membrane arm) |
Chemicals | ![]() ChemComp-CDL: ![]() ChemComp-PTY: ![]() ChemComp-NI: ![]() ChemComp-FCO: ![]() ChemComp-DR9: ![]() ChemComp-SF4: ![]() ChemComp-FE: ![]() ChemComp-LMN: ![]() ChemComp-HOH: ![]() ChemComp-MGD: ![]() ChemComp-6MO: |
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