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-Structure paper
| タイトル | The respiratory supercomplex from C. glutamicum. |
|---|---|
| ジャーナル・号・ページ | Structure, Vol. 30, Issue 3, Page 338-349.e3, Year 2022 |
| 掲載日 | 2022年3月3日 |
著者 | Agnes Moe / Terezia Kovalova / Sylwia Król / David J Yanofsky / Michael Bott / Dan Sjöstrand / John L Rubinstein / Martin Högbom / Peter Brzezinski / ![]() |
| PubMed 要旨 | Corynebacterium glutamicum is a preferentially aerobic gram-positive bacterium belonging to the phylum Actinobacteria, which also includes the pathogen Mycobacterium tuberculosis. In these bacteria, ...Corynebacterium glutamicum is a preferentially aerobic gram-positive bacterium belonging to the phylum Actinobacteria, which also includes the pathogen Mycobacterium tuberculosis. In these bacteria, respiratory complexes III and IV form a CIIICIV supercomplex that catalyzes oxidation of menaquinol and reduction of dioxygen to water. We isolated the C. glutamicum supercomplex and used cryo-EM to determine its structure at 2.9 Å resolution. The structure shows a central CIII dimer flanked by a CIV on two sides. A menaquinone is bound in each of the Q and Q sites in each CIII and an additional menaquinone is positioned ∼14 Å from heme b. A di-heme cyt. cc subunit electronically connects each CIII with an adjacent CIV, with the Rieske iron-sulfur protein positioned with the iron near heme b. Multiple subunits interact to form a convoluted sub-structure at the cytoplasmic side of the supercomplex, which defines a path for proton transfer into CIV. |
リンク | Structure / PubMed:34910901 |
| 手法 | EM (単粒子) |
| 解像度 | 2.9 Å |
| 構造データ | EMDB-13777, PDB-7q21: |
| 化合物 | ![]() ChemComp-CDL: ![]() ChemComp-7PH: ![]() ChemComp-TRD: ![]() ChemComp-9XX: ![]() ChemComp-TWT: ![]() ChemComp-CU: ![]() ChemComp-MG: ![]() ChemComp-HAS: ![]() ChemComp-CA: ![]() ChemComp-FES: ![]() ChemComp-MQ9: ![]() ChemComp-9YF: ![]() ChemComp-PLM: ![]() ChemComp-HEM: ![]() ChemComp-HEC: |
| 由来 |
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キーワード | ELECTRON TRANSPORT / MEMBRANE PROTEIN / CRYO-EM / RESPIRATORY SUPERCOMPLEX / ACTINOBACTERIA |
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corynebacterium glutamicum atcc 13032 (バクテリア)
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