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TitleThe respiratory supercomplex from C. glutamicum.
Journal, issue, pagesStructure, Vol. 30, Issue 3, Page 338-349.e3, Year 2022
Publish dateMar 3, 2022
AuthorsAgnes Moe / Terezia Kovalova / Sylwia Król / David J Yanofsky / Michael Bott / Dan Sjöstrand / John L Rubinstein / Martin Högbom / Peter Brzezinski /
PubMed AbstractCorynebacterium glutamicum is a preferentially aerobic gram-positive bacterium belonging to the phylum Actinobacteria, which also includes the pathogen Mycobacterium tuberculosis. In these bacteria, ...Corynebacterium glutamicum is a preferentially aerobic gram-positive bacterium belonging to the phylum Actinobacteria, which also includes the pathogen Mycobacterium tuberculosis. In these bacteria, respiratory complexes III and IV form a CIIICIV supercomplex that catalyzes oxidation of menaquinol and reduction of dioxygen to water. We isolated the C. glutamicum supercomplex and used cryo-EM to determine its structure at 2.9 Å resolution. The structure shows a central CIII dimer flanked by a CIV on two sides. A menaquinone is bound in each of the Q and Q sites in each CIII and an additional menaquinone is positioned ∼14 Å from heme b. A di-heme cyt. cc subunit electronically connects each CIII with an adjacent CIV, with the Rieske iron-sulfur protein positioned with the iron near heme b. Multiple subunits interact to form a convoluted sub-structure at the cytoplasmic side of the supercomplex, which defines a path for proton transfer into CIV.
External linksStructure / PubMed:34910901
MethodsEM (single particle)
Resolution2.9 Å
Structure data

13777

7q21

EMDB-13777, PDB-7q21:
III2-IV2 respiratory supercomplex from Corynebacterium glutamicum
Method: EM (single particle) / Resolution: 2.9 Å

Chemicals

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM / Cardiolipin

ChemComp-7PH:
(1R)-2-(dodecanoyloxy)-1-[(phosphonooxy)methyl]ethyl tetradecanoate / Phosphatidic acid

ChemComp-TRD:
TRIDECANE / Tridecane

ChemComp-9XX:
(2S)-1-(hexadecanoyloxy)propan-2-yl (10S)-10-methyloctadecanoate

ChemComp-TWT:
DOCOSANE / Higher alkanes

ChemComp-CU:
COPPER (II) ION / Copper

ChemComp-MG:
Unknown entry

ChemComp-HAS:
HEME-AS

ChemComp-CA:
Unknown entry

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER / Iron–sulfur cluster

ChemComp-MQ9:
MENAQUINONE-9 / Vitamin K2

ChemComp-9YF:
(2R)-2-(hexadecanoyloxy)-3-{[(S)-hydroxy{[(1R,2R,3R,4R,5R,6S)-2,3,4,5,6-pentahydroxycyclohexyl]oxy}phosphoryl]oxy}propyl (9S)-9-methyloctadecanoate

ChemComp-PLM:
PALMITIC ACID / Palmitic acid

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE / Heme B

ChemComp-HEC:
HEME C / Heme C

Source
  • corynebacterium glutamicum atcc 13032 (bacteria)
  • corynebacterium glutamicum (strain atcc 13032 / dsm 20300 / bcrc 11384 / jcm 1318 / lmg 3730 / ncimb 10025) (bacteria)
KeywordsELECTRON TRANSPORT / MEMBRANE PROTEIN / CRYO-EM / RESPIRATORY SUPERCOMPLEX / ACTINOBACTERIA

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