EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of a thylakoid-anchored contractile injection system in multicellular cyanobacteria.
ジャーナル・号・ページ	Nat Microbiol, Vol. 7, Issue 3, Page 386-396, Year 2022
掲載日	2022年2月14日
著者	Gregor L Weiss / Fabian Eisenstein / Ann-Katrin Kieninger / Jingwei Xu / Hannah A Minas / Milena Gerber / Miki Feldmüller / Iris Maldener / Karl Forchhammer / Martin Pilhofer /
PubMed 要旨	Contractile injection systems (CISs) mediate cell-cell interactions by phage tail-like structures, using two distinct modes of action: extracellular CISs are released into the medium, while type 6 ...Contractile injection systems (CISs) mediate cell-cell interactions by phage tail-like structures, using two distinct modes of action: extracellular CISs are released into the medium, while type 6 secretion systems (T6SSs) are attached to the cytoplasmic membrane and function upon cell-cell contact. Here, we characterized a CIS in the multicellular cyanobacterium Anabaena, with features distinct from extracellular CISs and T6SSs. Cryo-electron tomography of focused ion beam-milled cells revealed that CISs were anchored in thylakoid membrane stacks, facing the cell periphery. Single particle cryo-electron microscopy showed that this unique in situ localization was mediated by extensions of tail fibre and baseplate components. On stress, cyanobacteria induced the formation of ghost cells, presenting thylakoid-anchored CISs to the environment. Functional assays suggest that these CISs may mediate ghost cell formation and/or interactions of ghost cells with other organisms. Collectively, these data provide a framework for understanding the evolutionary re-engineering of CISs and potential roles of these CISs in cyanobacterial programmed cell death.
リンク	Nat Microbiol / PubMed:35165386 / PubMed Central
手法	EM (単粒子) / EM (トモグラフィー) / EM (サブトモグラム平均)
解像度	2.8 - 50.0 Å
構造データ	12029 7b5h EMDB-12029, PDB-7b5h: Cryo-EM structure of the contractile injection system base plate from Anabaena PCC7120 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-12030: Cryo-EM structure of the contractile injection system base plate from Anabaena PCC7120 (C6) 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-12031: Cryo-EM structure of the contractile injection system base plate connector from Anabaena PCC7120 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-12032: Cryo-EM structure of the contractile injection system crown trimer from Anabaena PCC7120 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-12033: Cryo-EM structure of the contractile injection system base plate in contracted form from Anabaena PCC7120 手法: EM (単粒子) / 解像度: 6.7 Å 12034 7b5i EMDB-12034, PDB-7b5i: Cryo-EM structure of the contractile injection system cap complex from Anabaena PCC7120 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-13770: Cryo-electron tomogram of cryo-FIB milled Anabaena PCC 7120 手法: EM (トモグラフィー) EMDB-13771: Cryo-electron tomogram of cryo-FIB milled Anabaena PCC 7120, cell periphery 手法: EM (トモグラフィー) EMDB-13772: Cryo-electron tomogram of cryo-FIB milled Anabaena PCC 7120 ghost cell 手法: EM (トモグラフィー) EMDB-13773: Subtomogram average of baseplate region of contractile injection systems in Anabaena PCC 7120 in extended state 手法: EM (サブトモグラム平均) / 解像度: 29.1 Å EMDB-13774: Subtomogram average of baseplate region of contractile injection systems in Anabaena PCC 7120 ghost cells in extended state 手法: EM (サブトモグラム平均) / 解像度: 37.2 Å EMDB-13775: Subtomogram average of baseplate region of contractile injection systems in Anabaena PCC 7120 ghost cells in contracted state 手法: EM (サブトモグラム平均) / 解像度: 50.0 Å
由来	Nostoc sp. PCC 7120 = FACHB-418 (バクテリア) nostoc sp. (strain pcc 7120 / sag 25.82 / utex 2576) (バクテリア)
キーワード	PROTEIN TRANSPORT / contractile tail / injection system / macromolecular machine / contractile protein