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TitleThe beginning of kinesin's force-generating cycle visualized at 9-A resolution.
Journal, issue, pagesJ Cell Biol, Vol. 177, Issue 3, Page 377-385, Year 2007
Publish dateMay 7, 2007
AuthorsCharles V Sindelar / Kenneth H Downing /
PubMed AbstractWe have used cryo-electron microscopy of kinesin-decorated microtubules to resolve the structure of the motor protein kinesin's crucial nucleotide response elements, switch I and the switch II helix, ...We have used cryo-electron microscopy of kinesin-decorated microtubules to resolve the structure of the motor protein kinesin's crucial nucleotide response elements, switch I and the switch II helix, in kinesin's poorly understood nucleotide-free state. Both of the switch elements undergo conformational change relative to the microtubule-free state. The changes in switch I suggest a role for it in "ejecting" adenosine diphosphate when kinesin initially binds to the microtubule. The switch II helix has an N-terminal extension, apparently stabilized by conserved microtubule contacts, implying a microtubule activation mechanism that could convey the state of the bound nucleotide to kinesin's putative force-delivering element (the "neck linker"). In deriving this structure, we have adapted an image-processing technique, single-particle reconstruction, for analyzing decorated microtubules. The resulting reconstruction visualizes the asymmetric seam present in native, 13-protofilament microtubules, and this method will provide an avenue to higher-resolution characterization of a variety of microtubule- binding proteins, as well as the microtubule itself.
External linksJ Cell Biol / PubMed:17470637 / PubMed Central
MethodsEM (single particle)
Resolution9.0 Å
Structure data

1340

2p4n

EMDB-1340: The beginning of kinesin's force-generating cycle visualized at 9-A resolution.
PDB-2p4n: Human Monomeric Kinesin (1BG2) and Bovine Tubulin (1JFF) Docked into the 9-Angstrom Cryo-EM Map of Nucleotide-Free Kinesin Complexed to the Microtubule
Method: EM (single particle) / Resolution: 9.0 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-ZN:
Unknown entry

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

ChemComp-TA1:
TAXOL / medication, chemotherapy*YM

Source
  • homo sapiens (human)
  • bos taurus (domestic cattle)
KeywordsTRANSPORT PROTEIN / Motor protein / ATPase

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