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TitleCryo-EM reconstructions of BMV-derived virus-like particles reveal assembly defects in the icosahedral lattice structure.
Journal, issue, pagesNanoscale, Vol. 14, Issue 8, Page 3224-3233, Year 2022
Publish dateFeb 24, 2022
AuthorsMilosz Ruszkowski / Aleksander Strugala / Paulina Indyka / Guillaume Tresset / Marek Figlerowicz / Anna Urbanowicz /
PubMed AbstractThe increasing interest in virus-like particles (VLPs) has been reflected by the growing number of studies on their assembly and application. However, the formation of complete VLPs is a complex ...The increasing interest in virus-like particles (VLPs) has been reflected by the growing number of studies on their assembly and application. However, the formation of complete VLPs is a complex phenomenon, making it difficult to rationally design VLPs with desired features . In this paper, we describe VLPs assembled from the recombinant capsid protein of brome mosaic virus (BMV). The analysis of VLPs was performed by Cryo-EM reconstructions and allowed us to visualize a few classes of VLPs, giving insight into the VLP self-assembly process. Apart from the mature icosahedral VLP practically identical with native virions, we describe putative VLP intermediates displaying non-icosahedral arrangements of capsomers, proposed to occur before the final disorder-order transition stage of icosahedral VLP assembly. Some of the described VLP classes show a lack of protein shell continuity, possibly resulting from too strong interaction with the cargo (in this case tRNA) with the capsid protein. We believe that our results are a useful prerequisite for the rational design of VLPs in the future and lead the way to the effective production of modified VLPs.
External linksNanoscale / PubMed:35156989
MethodsEM (single particle)
Resolution3.0 - 3.2 Å
Structure data

13344

7pe1

EMDB-13344, PDB-7pe1:
Cryo-EM structure of BMV-derived VLP expressed in E. coli and assembled in the presence of tRNA (tVLP)
Method: EM (single particle) / Resolution: 3.0 Å

13345

7pe2

EMDB-13345, PDB-7pe2:
Cryo-EM structure of BMV-derived VLP expressed in E. coli (eVLP)
Method: EM (single particle) / Resolution: 3.2 Å

Chemicals

ChemComp-HOH:
WATER

Source
  • brome mosaic virus
KeywordsVIRUS LIKE PARTICLE / BMV / brome mosaic virus / capsid proteins

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