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-Structure paper
Title | Following the signal sequence from ribosomal tunnel exit to signal recognition particle. |
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Journal, issue, pages | Nature, Vol. 444, Issue 7118, Page 507-511, Year 2006 |
Publish date | Nov 23, 2006 |
![]() | Mario Halic / Michael Blau / Thomas Becker / Thorsten Mielke / Martin R Pool / Klemens Wild / Irmgard Sinning / Roland Beckmann / ![]() |
PubMed Abstract | Membrane and secretory proteins can be co-translationally inserted into or translocated across the membrane. This process is dependent on signal sequence recognition on the ribosome by the signal ...Membrane and secretory proteins can be co-translationally inserted into or translocated across the membrane. This process is dependent on signal sequence recognition on the ribosome by the signal recognition particle (SRP), which results in targeting of the ribosome-nascent-chain complex to the protein-conducting channel at the membrane. Here we present an ensemble of structures at subnanometre resolution, revealing the signal sequence both at the ribosomal tunnel exit and in the bacterial and eukaryotic ribosome-SRP complexes. Molecular details of signal sequence interaction in both prokaryotic and eukaryotic complexes were obtained by fitting high-resolution molecular models. The signal sequence is presented at the ribosomal tunnel exit in an exposed position ready for accommodation in the hydrophobic groove of the rearranged SRP54 M domain. Upon ribosome binding, the SRP54 NG domain also undergoes a conformational rearrangement, priming it for the subsequent docking reaction with the NG domain of the SRP receptor. These findings provide the structural basis for improving our understanding of the early steps of co-translational protein sorting. |
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Methods | EM (single particle) |
Resolution | 8.7 - 10.7 Å |
Structure data | EMDB-1261: Following the signal sequence from ribosomal tunnel exit to signal recognition particle. EMDB-1262: Following the signal sequence from ribosomal tunnel exit to signal recognition particle. |
Chemicals | ![]() ChemComp-MG: ![]() ChemComp-HOH: |
Source |
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![]() | RIBOSOME / PROTEIN-RNA COMPLEX / SIGNAL RECOGNITION PARTICLE / SRP / TRANSLATION/RNA |