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TitleStructure of a full-length bacterial polysaccharide co-polymerase.
Journal, issue, pagesNat Commun, Vol. 12, Issue 1, Page 369, Year 2021
Publish dateJan 14, 2021
AuthorsBenjamin Wiseman / Ram Gopal Nitharwal / Göran Widmalm / Martin Högbom /
PubMed AbstractLipopolysaccharides are important components of the bacterial cell envelope that among other things act as a protective barrier against the environment and toxic molecules such as antibiotics. One of ...Lipopolysaccharides are important components of the bacterial cell envelope that among other things act as a protective barrier against the environment and toxic molecules such as antibiotics. One of the most widely disseminated pathways of polysaccharide biosynthesis is the inner membrane bound Wzy-dependent pathway. Here we present the 3.0 Å structure of the co-polymerase component of this pathway, WzzB from E. coli solved by single-particle cryo-electron microscopy. The overall architecture is octameric and resembles a box jellyfish containing a large bell-shaped periplasmic domain with the 2-helix transmembrane domain from each protomer, positioned 32 Å apart, encircling a large empty transmembrane chamber. This structure also reveals the architecture of the transmembrane domain, including the location of key residues for the Wzz-family of proteins and the Wzy-dependent pathway present in many Gram-negative bacteria, explaining several of the previous biochemical and mutational studies and lays the foundation for future investigations.
External linksNat Commun / PubMed:33446644 / PubMed Central
MethodsEM (single particle)
Resolution3.0 - 7.2 Å
Structure data

EMDB-11908:
N-terminally truncated WzzB from E.coli - C1 symmetry
Method: EM (single particle) / Resolution: 7.2 Å

EMDB-11909:
N-terminally truncated WzzB from E.coli - C8 symmetry
Method: EM (single particle) / Resolution: 4.5 Å

EMDB-4791:
full-length bacterial polysaccharide co-polymerase - C1 symmetry
Method: EM (single particle) / Resolution: 3.4 Å

4798

6rbg

EMDB-4798: full-length bacterial polysaccharide co-polymerase - C8 symmetry
PDB-6rbg: full-length bacterial polysaccharide co-polymerase
Method: EM (single particle) / Resolution: 3.0 Å

Source
  • Escherichia coli K-12 (bacteria)
  • escherichia coli (strain k12) (bacteria)
KeywordsMEMBRANE PROTEIN / enzyme / polysaccharide / co-polymerase

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