EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Elongational stalling activates mitoribosome-associated quality control.
ジャーナル・号・ページ	Science, Vol. 370, Issue 6520, Page 1105-1110, Year 2020
掲載日	2020年11月27日
著者	Nirupa Desai / Hanting Yang / Viswanathan Chandrasekaran / Razina Kazi / Michal Minczuk / V Ramakrishnan /
PubMed 要旨	The human mitochondrial ribosome (mitoribosome) and associated proteins regulate the synthesis of 13 essential subunits of the oxidative phosphorylation complexes. We report the discovery of a ...The human mitochondrial ribosome (mitoribosome) and associated proteins regulate the synthesis of 13 essential subunits of the oxidative phosphorylation complexes. We report the discovery of a mitoribosome-associated quality control pathway that responds to interruptions during elongation, and we present structures at 3.1- to 3.3-angstrom resolution of mitoribosomal large subunits trapped during ribosome rescue. Release factor homolog C12orf65 (mtRF-R) and RNA binding protein C6orf203 (MTRES1) eject the nascent chain and peptidyl transfer RNA (tRNA), respectively, from stalled ribosomes. Recruitment of mitoribosome biogenesis factors to these quality control intermediates suggests additional roles for these factors during mitoribosome rescue. We also report related cryo-electron microscopy structures (3.7 to 4.4 angstrom resolution) of elongating mitoribosomes bound to tRNAs, nascent polypeptides, the guanosine triphosphatase elongation factors mtEF-Tu and mtEF-G1, and the Oxa1L translocase.
リンク	Science / PubMed:33243891 / PubMed Central
手法	EM (単粒子)
解像度	3.1 - 4.4 Å
構造データ	EMDB-11636: Cryo-EM structure of the human mitoribosome in the rotated-1 state with A/A and P/E mt-tRNAs 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-11637: Cryo-EM structure of Oxa1L-bound human mitoribosomal large subunit 手法: EM (単粒子) / 解像度: 3.1 Å 11641 7a5f EMDB-11641, PDB-7a5f: Structure of the stalled human mitoribosome with P- and E-site mt-tRNAs 手法: EM (単粒子) / 解像度: 4.4 Å 11642 7a5g EMDB-11642, PDB-7a5g: Structure of the elongating human mitoribosome bound to mtEF-Tu.GMPPCP and A/T mt-tRNA 手法: EM (単粒子) / 解像度: 4.33 Å 11643 7a5h EMDB-11643, PDB-7a5h: Structure of the split human mitoribosomal large subunit with rescue factors mtRF-R and MTRES1 手法: EM (単粒子) / 解像度: 3.3 Å 11644 7a5i EMDB-11644, PDB-7a5i: Structure of the human mitoribosome with A- P-and E-site mt-tRNAs 手法: EM (単粒子) / 解像度: 3.7 Å 11645 7a5j EMDB-11645, PDB-7a5j: Structure of the split human mitoribosomal large subunit with P-and E-site mt-tRNAs 手法: EM (単粒子) / 解像度: 3.1 Å 11646 7a5k EMDB-11646, PDB-7a5k: Structure of the human mitoribosome in the post translocation state bound to mtEF-G1 手法: EM (単粒子) / 解像度: 3.7 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子YM ChemComp-SO4: SULFATE ION / 硫酸ジアニオン ChemComp-MLI: MALONATE ION / マロナ-ト ChemComp-CL: Unknown entry / クロリド ChemComp-NA: Unknown entry / ナトリウムカチオン ChemComp-HOH: WATER ChemComp-SPD: SPERMIDINE / スペルミジン ChemComp-GCP: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / グアニリルメチレンジホスホン酸 / GMP-PCP, エネルギー貯蔵分子類似体YM ChemComp-PNS: 4'-PHOSPHOPANTETHEINE / パンテテイン4′-りん酸
由来	homo sapiens (ヒト) thermus thermophilus (strain hb27 / atcc baa-163 / dsm 7039) (バクテリア) human (ヒト) thermus thermophilus hb27 (バクテリア)
キーワード	RIBOSOME / mitochondrial ribosome / ribosome stalling / cryo-EM