EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	The MscS-like channel YnaI has a gating mechanism based on flexible pore helices.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 117, Issue 46, Page 28754-28762, Year 2020
掲載日	2020年11月17日
著者	Vanessa Judith Flegler / Akiko Rasmussen / Shanlin Rao / Na Wu / Renato Zenobi / Mark S P Sansom / Rainer Hedrich / Tim Rasmussen / Bettina Böttcher /
PubMed 要旨	The mechanosensitive channel of small conductance (MscS) is the prototype of an evolutionarily diversified large family that fine-tunes osmoregulation but is likely to fulfill additional functions. ...The mechanosensitive channel of small conductance (MscS) is the prototype of an evolutionarily diversified large family that fine-tunes osmoregulation but is likely to fulfill additional functions. has six osmoprotective paralogs with different numbers of transmembrane helices. These helices are important for gating and sensing in MscS but the role of the additional helices in the paralogs is not understood. The medium-sized channel YnaI was extracted and delivered in native nanodiscs in closed-like and open-like conformations using the copolymer diisobutylene/maleic acid (DIBMA) for structural studies. Here we show by electron cryomicroscopy that YnaI has an extended sensor paddle that during gating relocates relative to the pore concomitant with bending of a GGxGG motif in the pore helices. YnaI is the only one of the six paralogs that has this GGxGG motif allowing the sensor paddle to move outward. Access to the pore is through a vestibule on the cytosolic side that is fenestrated by side portals. In YnaI, these portals are obstructed by aromatic side chains but are still fully hydrated and thus support conductance. For comparison with large-sized channels, we determined the structure of YbiO, which showed larger portals and a wider pore with no GGxGG motif. Further in silico comparison of MscS, YnaI, and YbiO highlighted differences in the hydrophobicity and wettability of their pores and vestibule interiors. Thus, MscS-like channels of different sizes have a common core architecture but show different gating mechanisms and fine-tuned conductive properties.
リンク	Proc Natl Acad Sci U S A / PubMed:33148804 / PubMed Central
手法	EM (単粒子)
解像度	3.0 - 4.2 Å
構造データ	EMDB-11556: small conductance mechanosensitive channel YnaI mutant G149A/G152A 手法: EM (単粒子) / 解像度: 4.2 Å 11557 6zyd EMDB-11557, PDB-6zyd: YnaI 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-11558: mechanosensitive channel YnaI in amphipol A8-35 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-11559: mechanosensitive channel YnaI in a closed-like conformation 手法: EM (単粒子) / 解像度: 3.8 Å 11560 6zye EMDB-11560, PDB-6zye: YnaI in an open-like conformation 手法: EM (単粒子) / 解像度: 4.1 Å EMDB-11561: mechanosensitive channel YnaI in an intermediate conformation 手法: EM (単粒子) / 解像度: 3.32 Å 11629 7a46 EMDB-11629, PDB-7a46: small conductance mechanosensitive channel YbiO 手法: EM (単粒子) / 解像度: 3.0 Å
化合物	ChemComp-PEE: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine / DOPE, リン脂質*YM / Discrete optimized protein energy
由来	escherichia coli (大腸菌) escherichia coli (strain k12) (大腸菌)
キーワード	MEMBRANE PROTEIN (膜タンパク質) / YnaI small-conductance mechanosensitive channel DIBMALPs / YnaI / small-conductance mechanosensitive channel / DIBMALPs / YbiO / mechanosensitive channel / MscS-like channel / E. coli (大腸菌)