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TitleNanoscale architecture of a VAP-A-OSBP tethering complex at membrane contact sites.
Journal, issue, pagesNat Commun, Vol. 12, Issue 1, Page 3459, Year 2021
Publish dateJun 8, 2021
AuthorsEugenio de la Mora / Manuela Dezi / Aurélie Di Cicco / Joëlle Bigay / Romain Gautier / John Manzi / Joël Polidori / Daniel Castaño-Díez / Bruno Mesmin / Bruno Antonny / Daniel Lévy /
PubMed AbstractMembrane contact sites (MCS) are subcellular regions where two organelles appose their membranes to exchange small molecules, including lipids. Structural information on how proteins form MCS is ...Membrane contact sites (MCS) are subcellular regions where two organelles appose their membranes to exchange small molecules, including lipids. Structural information on how proteins form MCS is scarce. We designed an in vitro MCS with two membranes and a pair of tethering proteins suitable for cryo-tomography analysis. It includes VAP-A, an ER transmembrane protein interacting with a myriad of cytosolic proteins, and oxysterol-binding protein (OSBP), a lipid transfer protein that transports cholesterol from the ER to the trans Golgi network. We show that VAP-A is a highly flexible protein, allowing formation of MCS of variable intermembrane distance. The tethering part of OSBP contains a central, dimeric, and helical T-shape region. We propose that the molecular flexibility of VAP-A enables the recruitment of partners of different sizes within MCS of adjustable thickness, whereas the T geometry of the OSBP dimer facilitates the movement of the two lipid-transfer domains between membranes.
External linksNat Commun / PubMed:34103503 / PubMed Central
MethodsEM (subtomogram averaging) / EM (tomography)
Resolution9.8 - 25.2 Å
Structure data

EMDB-11376:
Subtomogram averaging of the OSBP construct NPHFFAT.
Method: EM (subtomogram averaging) / Resolution: 9.8 Å

EMDB-11399:
VAP-A engaged in in vitro membrane contact sites.
Method: EM (subtomogram averaging) / Resolution: 25.2 Å

EMDB-11402:
VAP-A engaged in in vitro membrane contact sites with the OSBP construct N-PH-FFAT at 19.6 A resolution
Method: EM (subtomogram averaging) / Resolution: 19.6 Å

EMDB-11427:
Representative tomogram of in vitro membrane contact sites between VAP-A and N-PH-FFAT
Method: EM (tomography)

EMDB-11438:
Representative tomogram of in vitro membrane contact sites between VAP-A and N-PH-FFAT. Example of VAP-A reconstituted at high concentration.
Method: EM (tomography)

EMDB-11455:
Representative tomogram of in vitro membrane contact sites between VAP-A and OSBP.
Method: EM (tomography)

Source
  • Homo sapiens (human)

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