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-Structure paper
| Title | Structure of the TFIIIC subcomplex τA provides insights into RNA polymerase III pre-initiation complex formation. |
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| Journal, issue, pages | Nat Commun, Vol. 11, Issue 1, Page 4905, Year 2020 |
| Publish date | Sep 30, 2020 |
 Authors | Matthias K Vorländer / Anna Jungblut / Kai Karius / Florence Baudin / Helga Grötsch / Jan Kosinski / Christoph W Müller /  |
| PubMed Abstract | Transcription factor (TF) IIIC is a conserved eukaryotic six-subunit protein complex with dual function. It serves as a general TF for most RNA polymerase (Pol) III genes by recruiting TFIIIB, but it ...Transcription factor (TF) IIIC is a conserved eukaryotic six-subunit protein complex with dual function. It serves as a general TF for most RNA polymerase (Pol) III genes by recruiting TFIIIB, but it is also involved in chromatin organization and regulation of Pol II genes through interaction with CTCF and condensin II. Here, we report the structure of the S. cerevisiae TFIIIC subcomplex τA, which contains the most conserved subunits of TFIIIC and is responsible for recruitment of TFIIIB and transcription start site (TSS) selection at Pol III genes. We show that τA binding to its promoter is auto-inhibited by a disordered acidic tail of subunit τ95. We further provide a negative-stain reconstruction of τA bound to the TFIIIB subunits Brf1 and TBP. This shows that a ruler element in τA achieves positioning of TFIIIB upstream of the TSS, and suggests remodeling of the complex during assembly of TFIIIB by TFIIIC. |
 External links | Nat Commun / PubMed:32999288 / PubMed Central |
| Methods | EM (single particle) |
| Resolution | 3.1 - 32.36 Å |
| Structure data |  EMDB-10795: EMDB-10817, PDB-6yj6: |
| Source |
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 Keywords | TRANSCRIPTION / TFIIIC / tauA / Transcription initiation / Pol III / TFIIIB / Transcription factor |
saccharomyces cerevisiae (brewer's yeast)