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-Structure paper
Title | Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation. |
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Journal, issue, pages | EMBO J, Vol. 23, Issue 5, Page 1008-1019, Year 2004 |
Publish date | Mar 10, 2004 |
Authors | Christian M T Spahn / Maria G Gomez-Lorenzo / Robert A Grassucci / Rene Jørgensen / Gregers R Andersen / Roland Beckmann / Pawel A Penczek / Juan P G Ballesta / Joachim Frank / |
PubMed Abstract | An 11.7-A-resolution cryo-EM map of the yeast 80S.eEF2 complex in the presence of the antibiotic sordarin was interpreted in molecular terms, revealing large conformational changes within eEF2 and ...An 11.7-A-resolution cryo-EM map of the yeast 80S.eEF2 complex in the presence of the antibiotic sordarin was interpreted in molecular terms, revealing large conformational changes within eEF2 and the 80S ribosome, including a rearrangement of the functionally important ribosomal intersubunit bridges. Sordarin positions domain III of eEF2 so that it can interact with the sarcin-ricin loop of 25S rRNA and protein rpS23 (S12p). This particular conformation explains the inhibitory action of sordarin and suggests that eEF2 is stalled on the 80S ribosome in a conformation that has similarities with the GTPase activation state. A ratchet-like subunit rearrangement (RSR) occurs in the 80S.eEF2.sordarin complex that, in contrast to Escherichia coli 70S ribosomes, is also present in vacant 80S ribosomes. A model is suggested, according to which the RSR is part of a mechanism for moving the tRNAs during the translocation reaction. |
External links | EMBO J / PubMed:14976550 / PubMed Central |
Methods | EM (single particle) |
Resolution | 11.7 Å |
Structure data | EMDB-1067: Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation. |
Source |
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Keywords | RIBOSOME / 80S ribosome / 40S ribosomal subunit / eEF2 / tRNA translocation / sordarin / cryo-EM |