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TitleStructure of nucleosome-bound human BAF complex.
Journal, issue, pagesScience, Vol. 367, Issue 6480, Page 875-881, Year 2020
Publish dateFeb 21, 2020
AuthorsShuang He / Zihan Wu / Yuan Tian / Zishuo Yu / Jiali Yu / Xinxin Wang / Jie Li / Bijun Liu / Yanhui Xu /
PubMed AbstractMammalian SWI/SNF family chromatin remodelers, BRG1/BRM-associated factor (BAF) and polybromo-associated BAF (PBAF), regulate chromatin structure and transcription, and their mutations are linked to ...Mammalian SWI/SNF family chromatin remodelers, BRG1/BRM-associated factor (BAF) and polybromo-associated BAF (PBAF), regulate chromatin structure and transcription, and their mutations are linked to cancers. The 3.7-angstrom-resolution cryo-electron microscopy structure of human BAF bound to the nucleosome reveals that the nucleosome is sandwiched by the base and the adenosine triphosphatase (ATPase) modules, which are bridged by the actin-related protein (ARP) module. The ATPase motor is positioned proximal to nucleosomal DNA and, upon ATP hydrolysis, engages with and pumps DNA along the nucleosome. The C-terminal α helix of SMARCB1, enriched in positively charged residues frequently mutated in cancers, mediates interactions with an acidic patch of the nucleosome. AT-rich interactive domain-containing protein 1A (ARID1A) and the SWI/SNF complex subunit SMARCC serve as a structural core and scaffold in the base module organization, respectively. Our study provides structural insights into subunit organization and nucleosome recognition of human BAF complex.
External linksScience / PubMed:32001526
MethodsEM (single particle)
Resolution3.0 - 10.3 Å
Structure data

0968

6lth

EMDB-0968, PDB-6lth:
Structure of human BAF Base module
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-0969:
Structure of human BAF ARP module
Method: EM (single particle) / Resolution: 4.2 Å

EMDB-0970:
Structure of nucleosome in BAF-NCP complex
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-0971:
Structure of nucleosome-bound human full length BAF complex
Method: EM (single particle) / Resolution: 6.6 Å

EMDB-0972:
Structure of nucleosome with DNA detachment in BAF-NCP complex
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-0973:
Structure of nucleosome-bound human BAF complex in ADP-bound state
Method: EM (single particle) / Resolution: 10.3 Å

0974

6ltj

EMDB-0974, PDB-6ltj:
Structure of nucleosome-bound human BAF complex
Method: EM (single particle) / Resolution: 3.7 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • homo sapiens (human)
  • Xenopus calcaratus (Biafran clawed frog)
  • xenopus laevis (African clawed frog)
KeywordsGENE REGULATION / Chromatin remodeler / Complex

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