EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of ATP synthase from an early photosynthetic bacterium .
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 122, Issue 13, Page e2425824122, Year 2025
掲載日	2025年3月25日
著者	Xin Zhang / Jingyi Wu / Zhenzhen Min / Jiamao Wang / Xin Hong / Xinkai Pei / Zihe Rao / Xiaoling Xu /
PubMed 要旨	F-type ATP synthase (FF) catalyzes proton motive force-driven ATP synthesis in mitochondria, chloroplasts, and bacteria. Different from the mitochondrial and bacterial enzymes, FF from photosynthetic ...F-type ATP synthase (FF) catalyzes proton motive force-driven ATP synthesis in mitochondria, chloroplasts, and bacteria. Different from the mitochondrial and bacterial enzymes, FF from photosynthetic organisms have evolved diverse structural and mechanistic details to adapt to the light-dependent reactions. Although complete structure of chloroplast FF has been reported, no high-resolution structure of an FF from photosynthetic bacteria has been available. Here, we report cryo-EM structures of an intact and functionally competent FF from (FF), a filamentous anoxygenic phototrophic bacterium from the earliest branch of photosynthetic organisms. The structures of FF in its ADP-free and ADP-bound forms for three rotational states reveal a previously unrecognized architecture of ATP synthases. A pair of peripheral stalks connect to the F head through a dimer of δ-subunits, and associate with two membrane-embedded a-subunits that are asymmetrically positioned outside and clamp F's c-ring. The two a-subunits constitute two proton inlets on the periplasmic side and two proton outlets on the cytoplasmic side, endowing FF with unique proton translocation pathways that allow more protons being translocated relative to single a-subunit FF. Our findings deepen understanding of the architecture and proton translocation mechanisms of FF synthases and suggest innovative strategies for modulating their activities by altering the number of a-subunit.
リンク	Proc Natl Acad Sci U S A / PubMed:40131952 / PubMed Central
手法	EM (単粒子)
解像度	2.84 - 5.18 Å
構造データ	60868 9itj EMDB-60868, PDB-9itj: Chloroflexus aurantiacus ATP synthase, state 1 手法: EM (単粒子) / 解像度: 2.84 Å 60869 9itk EMDB-60869, PDB-9itk: Chloroflexus aurantiacus ATP synthase, state 2 手法: EM (単粒子) / 解像度: 2.89 Å 60870 9itl EMDB-60870, PDB-9itl: Chloroflexus aurantiacus ATP synthase, state 3 手法: EM (単粒子) / 解像度: 3.31 Å 60871 9itm EMDB-60871, PDB-9itm: Chloroflexus aurantiacus ATP synthase, state 1, focused refinement of FO 手法: EM (単粒子) / 解像度: 3.16 Å 60872 9itn EMDB-60872, PDB-9itn: Chloroflexus aurantiacus ATP synthase, state 1, focused refinement of FO and peripheral stalk 手法: EM (単粒子) / 解像度: 3.48 Å 60873 9ito EMDB-60873, PDB-9ito: Chloroflexus aurantiacus ATP synthase, state 2, focused refinement of FO 手法: EM (単粒子) / 解像度: 3.3 Å 60874 9itp EMDB-60874, PDB-9itp: Chloroflexus aurantiacus ATP synthase, state 2, focused refinement of FO and peripheral stalk 手法: EM (単粒子) / 解像度: 3.85 Å 60875 9itq EMDB-60875, PDB-9itq: Chloroflexus aurantiacus ATP synthase, state 3, focused refinement of FO 手法: EM (単粒子) / 解像度: 3.98 Å 60876 9itr EMDB-60876, PDB-9itr: Chloroflexus aurantiacus ATP synthase, state 3, focused refinement of FO and peripheral stalk 手法: EM (単粒子) / 解像度: 4.6 Å 60877 9its EMDB-60877, PDB-9its: Chloroflexus aurantiacus ADP-bound ATP synthase, state 1 手法: EM (単粒子) / 解像度: 2.89 Å 60878 9itt EMDB-60878, PDB-9itt: Chloroflexus aurantiacus ADP-bound ATP synthase, state 2 手法: EM (単粒子) / 解像度: 2.96 Å 60879 9itu EMDB-60879, PDB-9itu: Chloroflexus aurantiacus ADP-bound ATP synthase, state 3 手法: EM (単粒子) / 解像度: 3.18 Å 60880 9itv EMDB-60880, PDB-9itv: Chloroflexus aurantiacus ADP-bound ATP synthase, state 1, focused refinement of FO 手法: EM (単粒子) / 解像度: 3.97 Å 60881 9itw EMDB-60881, PDB-9itw: Chloroflexus aurantiacus ADP-bound ATP synthase, state 1, focused refinement of FO and peripheral stalk 手法: EM (単粒子) / 解像度: 4.08 Å 60882 9itx EMDB-60882, PDB-9itx: Chloroflexus aurantiacus ADP-bound ATP synthase, state 2, focused refinement of FO 手法: EM (単粒子) / 解像度: 4.1 Å 60883 9ity EMDB-60883, PDB-9ity: Chloroflexus aurantiacus ADP-bound ATP synthase, state 2, focused refinement of FO and peripheral stalk 手法: EM (単粒子) / 解像度: 4.95 Å 60884 9itz EMDB-60884, PDB-9itz: Chloroflexus aurantiacus ADP-bound ATP synthase, state 3, focused refinement of FO 手法: EM (単粒子) / 解像度: 4.28 Å 60885 9iu0 EMDB-60885, PDB-9iu0: Chloroflexus aurantiacus ADP-bound ATP synthase, state 3, focused refinement of FO and peripheral stalk 手法: EM (単粒子) / 解像度: 5.18 Å
化合物	ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-PO4: PHOSPHATE ION / ホスファ-ト
由来	chloroflexus aurantiacus j-10-fl (バクテリア)
キーワード	MEMBRANE PROTEIN / ATP synthesis / proton channels / proton-motive force / proton translocation