EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis for processive daughter-strand synthesis and proofreading by the human leading-strand DNA polymerase Pol ε.
ジャーナル・号・ページ	Nat Struct Mol Biol, Year 2024
掲載日	2024年8月7日
著者	Johann J Roske / Joseph T P Yeeles /
PubMed 要旨	During chromosome replication, the nascent leading strand is synthesized by DNA polymerase epsilon (Pol ε), which associates with the sliding clamp processivity factor proliferating cell nuclear ...During chromosome replication, the nascent leading strand is synthesized by DNA polymerase epsilon (Pol ε), which associates with the sliding clamp processivity factor proliferating cell nuclear antigen (PCNA) to form a processive holoenzyme. For high-fidelity DNA synthesis, Pol ε relies on nucleotide selectivity and its proofreading ability to detect and excise a misincorporated nucleotide. Here, we present cryo-electron microscopy (cryo-EM) structures of human Pol ε in complex with PCNA, DNA and an incoming nucleotide, revealing how Pol ε associates with PCNA through its PCNA-interacting peptide box and additional unique features of its catalytic domain. Furthermore, by solving a series of cryo-EM structures of Pol ε at a mismatch-containing DNA, we elucidate how Pol ε senses and edits a misincorporated nucleotide. Our structures delineate steps along an intramolecular switching mechanism between polymerase and exonuclease activities, providing the basis for a proofreading mechanism in B-family replicative polymerases.
リンク	Nat Struct Mol Biol / PubMed:39112807
手法	EM (単粒子)
解像度	3.3 - 4.2 Å
構造データ	50222 9f6d EMDB-50222, PDB-9f6d: Human DNA polymerase epsilon bound to DNA and PCNA (open conformation) 手法: EM (単粒子) / 解像度: 3.6 Å 50223 9f6e EMDB-50223, PDB-9f6e: Human DNA polymerase epsilon bound to DNA and PCNA (ajar conformation) 手法: EM (単粒子) / 解像度: 3.74 Å 50224 9f6f EMDB-50224, PDB-9f6f: Human DNA polymerase epsilon bound to DNA and PCNA (closed conformation) 手法: EM (単粒子) / 解像度: 3.75 Å 50225 9f6i EMDB-50225, PDB-9f6i: Human DNA Polymerase epsilon bound to T-C mismatched DNA (Post-Insertion state) 手法: EM (単粒子) / 解像度: 3.3 Å 50226 9f6j EMDB-50226, PDB-9f6j: Human DNA Polymerase epsilon bound to T-C mismatched DNA (Polymerase Arrest state) 手法: EM (単粒子) / 解像度: 3.9 Å 50227 9f6k EMDB-50227, PDB-9f6k: Human DNA Polymerase epsilon bound to T-C mismatched DNA (Frayed Substrate state) 手法: EM (単粒子) / 解像度: 4.2 Å 50228 9f6l EMDB-50228, PDB-9f6l: Human DNA Polymerase epsilon bound to T-C mismatched DNA (Mismatch Excision state) 手法: EM (単粒子) / 解像度: 3.9 Å
化合物	ChemComp-SF4: IRON/SULFUR CLUSTER ChemComp-DDS: 2',3'-dideoxyadenosine triphosphate / ddATP ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-CA: Unknown entry / カルシウムジカチオン
由来	homo sapiens (ヒト) synthetic construct (人工物)
キーワード	REPLICATION / DNA / polymerase / epsilon / PCNA / leading strand / human / replisome / proofreading