EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Blueprinting extendable nanomaterials with standardized protein blocks.
ジャーナル・号・ページ	Nature, Vol. 627, Issue 8005, Page 898-904, Year 2024
掲載日	2024年3月13日
著者	Timothy F Huddy / Yang Hsia / Ryan D Kibler / Jinwei Xu / Neville Bethel / Deepesh Nagarajan / Rachel Redler / Philip J Y Leung / Connor Weidle / Alexis Courbet / Erin C Yang / Asim K Bera / Nicolas Coudray / S John Calise / Fatima A Davila-Hernandez / Hannah L Han / Kenneth D Carr / Zhe Li / Ryan McHugh / Gabriella Reggiano / Alex Kang / Banumathi Sankaran / Miles S Dickinson / Brian Coventry / T J Brunette / Yulai Liu / Justas Dauparas / Andrew J Borst / Damian Ekiert / Justin M Kollman / Gira Bhabha / David Baker /
PubMed 要旨	A wooden house frame consists of many different lumber pieces, but because of the regularity of these building blocks, the structure can be designed using straightforward geometrical principles. The ...A wooden house frame consists of many different lumber pieces, but because of the regularity of these building blocks, the structure can be designed using straightforward geometrical principles. The design of multicomponent protein assemblies, in comparison, has been much more complex, largely owing to the irregular shapes of protein structures. Here we describe extendable linear, curved and angled protein building blocks, as well as inter-block interactions, that conform to specified geometric standards; assemblies designed using these blocks inherit their extendability and regular interaction surfaces, enabling them to be expanded or contracted by varying the number of modules, and reinforced with secondary struts. Using X-ray crystallography and electron microscopy, we validate nanomaterial designs ranging from simple polygonal and circular oligomers that can be concentrically nested, up to large polyhedral nanocages and unbounded straight 'train track' assemblies with reconfigurable sizes and geometries that can be readily blueprinted. Because of the complexity of protein structures and sequence-structure relationships, it has not previously been possible to build up large protein assemblies by deliberate placement of protein backbones onto a blank three-dimensional canvas; the simplicity and geometric regularity of our design platform now enables construction of protein nanomaterials according to 'back of an envelope' architectural blueprints.
リンク	Nature / PubMed:38480887 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2.48 - 12.98 Å
構造データ	29974 8gel EMDB-29974, PDB-8gel: Cryo-EM structure of synthetic tetrameric building block sC4 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-40070: Cryo-EM map of synthetic cage_O3_10 reconstructed without symmetry (C1) 手法: EM (単粒子) / 解像度: 7.4 Å EMDB-40071: Cryo-EM map of synthetic cage_O3_10 reconstructed with O symmetry 手法: EM (単粒子) / 解像度: 6.0 Å EMDB-40073: Cryo-EM map of synthetic cage_T3_5 reconstructed without symmetry (C1), with 1 monomer missing (class 3.0) 手法: EM (単粒子) / 解像度: 6.1 Å EMDB-40074: Cryo-EM map of synthetic cage_T3_5 reconstructed with T symmetry 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-40075: Cryo-EM map of synthetic cage_T3_5 reconstructed without symmetry (C1) 手法: EM (単粒子) / 解像度: 4.3 Å EMDB-40076: Cryo-EM map of synthetic cage_T3_5+2 reconstructed without symmetry (C1) 手法: EM (単粒子) / 解像度: 6.7 Å 41364 8tl7 EMDB-41364, PDB-8tl7: CryoEM Structure of a Computationally Designed T3 Tetrahedral Nanocage 手法: EM (単粒子) / 解像度: 4.05 Å EMDB-41907: Computationally Designed, Expandable O4 Octahedral Handshake Nanocage 手法: EM (単粒子) / 解像度: 6.34 Å EMDB-42031: Computational Designed Nanocage O43_129_+8 手法: EM (単粒子) / 解像度: 12.98 Å 42906 8v2d EMDB-42906, PDB-8v2d: Computational Designed Nanocage O43_129 手法: EM (単粒子) / 解像度: 6.77 Å 42944 8v3b EMDB-42944, PDB-8v3b: Computational Designed Nanocage O43_129_+4 手法: EM (単粒子) / 解像度: 6.4 Å EMDB-43318: Twistless helix 12 repeat ring design R12B 手法: EM (単粒子) / 解像度: 5.2 Å PDB-8g9j: Geometrically programmable nanomaterial construction using regularized protein building blocks 手法: X-RAY DIFFRACTION / 解像度: 2.5 Å PDB-8g9k: Geometrically programmable nanomaterial construction using regularized protein building blocks 手法: X-RAY DIFFRACTION / 解像度: 2.48 Å PDB-8ga6: Geometrically programmable nanomaterial construction using regularized protein building blocks 手法: X-RAY DIFFRACTION / 解像度: 2.5 Å PDB-8ga7: Geometrically programmable nanomaterial construction using regularized protein building blocks 手法: X-RAY DIFFRACTION / 解像度: 2.93 Å
化合物	ChemComp-HOH: WATER
由来	synthetic construct (人工物) unidentified (未定義)
キーワード	DE NOVO PROTEIN / nanomaterial / protein building blocks / De novo design / train-track / synthetic / tetramer / self-assembling / expandable nanomaterial / de novo / t3 tetrahedral nanocage / computationally designed / nanocage / expandable nanomaterials / O43_129 / extendable nanomaterials / O43 / Programmable Design