タイトル | Escherichia coli transcription-translation coupled complex class A (TTC-A) containing RfaH bound to ops signal, mRNA with a 21 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome |
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ジャーナル・号・ページ | Nat. Struct. Mol. Biol., Year 2024 |
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掲載日 | 2023年10月23日 (構造データの登録日) |
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著者 | Molodtsov V / Wang C / Zhang J / Kaelber JT / Blaha G / Ebright RH |
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リンク | PubMedで検索 |
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手法 | EM (単粒子) |
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解像度 | 3.2 - 6.6 Å |
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構造データ | EMDB-42453, PDB-8upo: Escherichia coli transcription-translation coupled complex class A (TTC-A) containing RfaH bound to ops signal, mRNA with a 21 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome 手法: EM (単粒子) / 解像度: 5.5 Å EMDB-42454, PDB-8upr: Escherichia coli transcription-translation coupled complex class A (TTC-A) containing RfaH bound to ops signal, mRNA with a 21 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome 手法: EM (単粒子) / 解像度: 5.3 Å EMDB-42473, PDB-8uql: Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH in loaded state, mRNA with a 24 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-42474, PDB-8uqm: Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH in loaded state, NusA, mRNA with a 24 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome 手法: EM (単粒子) / 解像度: 5.3 Å EMDB-42477, PDB-8uqp: Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, mRNA with a 24 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-42479, PDB-8ur0: Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, NusA, mRNA with a 24 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-42492, PDB-8urh: Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, mRNA with a 27 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome 手法: EM (単粒子) / 解像度: 5.7 Å EMDB-42493, PDB-8uri: Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, NusA, mRNA with a 27 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome 手法: EM (単粒子) / 解像度: 5.3 Å EMDB-42503, PDB-8urx: Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, mRNA with a 30 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome 手法: EM (単粒子) / 解像度: 6.6 Å |
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化合物 | |
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由来 | ![](img/tx_bacteria.gif) escherichia coli (大腸菌)- synthetic construct (人工物)
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キーワード | TRANSCRIPTION / translation / RfaH / gene expression / regulation / ops / ribosome / coupling |
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