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- EMDB-42479: Escherichia coli transcription-translation coupled complex class ... -
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Open data
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Basic information
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Title | Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, NusA, mRNA with a 24 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome | |||||||||
![]() | Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, NusA, mRNA with a 24 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome | |||||||||
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![]() | transcription / translation / RfaH / gene expression / regulation / ops / ribosome / coupling | |||||||||
Function / homology | ![]() transcription antitermination factor activity, DNA binding / negative regulation of cytoplasmic translational initiation / transcription elongation-coupled chromatin remodeling / RNA polymerase complex / submerged biofilm formation / cellular response to cell envelope stress / cytosolic DNA-directed RNA polymerase complex / stringent response / regulation of DNA-templated transcription initiation / bacterial-type flagellum assembly ...transcription antitermination factor activity, DNA binding / negative regulation of cytoplasmic translational initiation / transcription elongation-coupled chromatin remodeling / RNA polymerase complex / submerged biofilm formation / cellular response to cell envelope stress / cytosolic DNA-directed RNA polymerase complex / stringent response / regulation of DNA-templated transcription initiation / bacterial-type flagellum assembly / misfolded RNA binding / Group I intron splicing / RNA folding / bacterial-type flagellum-dependent cell motility / positive regulation of ribosome biogenesis / nitrate assimilation / translational termination / DnaA-L2 complex / negative regulation of translational initiation / negative regulation of DNA-templated DNA replication initiation / translational initiation / ribosome assembly / mRNA regulatory element binding translation repressor activity / positive regulation of RNA splicing / assembly of large subunit precursor of preribosome / transcription elongation factor complex / regulation of DNA-templated transcription elongation / cytosolic ribosome assembly / transcription antitermination / DNA-templated transcription initiation / cell motility / regulation of cell growth / DNA-templated transcription termination / maintenance of translational fidelity / ribosomal large subunit assembly / ribonucleoside binding / mRNA 5'-UTR binding / DNA-directed 5'-3' RNA polymerase activity / DNA-directed RNA polymerase / large ribosomal subunit / ribosome binding / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / transferase activity / 5S rRNA binding / response to heat / large ribosomal subunit rRNA binding / protein-containing complex assembly / cytosolic small ribosomal subunit / intracellular iron ion homeostasis / cytosolic large ribosomal subunit / tRNA binding / cytoplasmic translation / protein dimerization activity / rRNA binding / negative regulation of translation / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / DNA-binding transcription factor activity / response to antibiotic / nucleotide binding / mRNA binding / magnesium ion binding / DNA binding / RNA binding / zinc ion binding / membrane / cytoplasm / cytosol Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.4 Å | |||||||||
![]() | Molodtsov V / Wang C / Ebright RH | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Escherichia coli transcription-translation coupled complex class A (TTC-A) containing RfaH bound to ops signal, mRNA with a 21 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Authors: Molodtsov V / Wang C / Zhang J / Kaelber JT / Blaha G / Ebright RH | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 196.5 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 94.6 KB 94.6 KB | Display Display | ![]() |
Images | ![]() | 37.6 KB | ||
Filedesc metadata | ![]() | 20.1 KB | ||
Others | ![]() ![]() | 585.3 MB 588.7 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 1 MB | Display | ![]() |
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Full document | ![]() | 1 MB | Display | |
Data in XML | ![]() | 21 KB | Display | |
Data in CIF | ![]() | 23.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8ur0MC ![]() 8upoC ![]() 8uprC ![]() 8uqlC ![]() 8uqmC ![]() 8uqpC ![]() 8urhC ![]() 8uriC ![]() 8urxC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Annotation | Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, NusA, mRNA with a 24 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.069 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: half-map 2
File | emd_42479_half_map_1.map | ||||||||||||
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Annotation | half-map 2 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: half-map 1
File | emd_42479_half_map_2.map | ||||||||||||
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Annotation | half-map 1 | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
+Entire : Escherichia coli transcription-translation coupled complex class ...
+Supramolecule #1: Escherichia coli transcription-translation coupled complex class ...
+Macromolecule #1: Ribosomal protein L21
+Macromolecule #2: 50S ribosomal protein L22
+Macromolecule #3: 50S ribosomal protein L23
+Macromolecule #4: 50S ribosomal protein L24
+Macromolecule #5: 50S ribosomal protein L25
+Macromolecule #9: 50S ribosomal protein L10
+Macromolecule #11: DNA-directed RNA polymerase subunit beta
+Macromolecule #12: Transcription antitermination protein RfaH
+Macromolecule #13: DNA-directed RNA polymerase subunit alpha
+Macromolecule #14: DNA-directed RNA polymerase subunit beta'
+Macromolecule #15: DNA-directed RNA polymerase subunit omega
+Macromolecule #16: Transcription termination/antitermination protein NusA
+Macromolecule #17: 30S ribosomal protein S18
+Macromolecule #19: 30S ribosomal protein S20
+Macromolecule #20: 30S ribosomal protein S21
+Macromolecule #21: 30S ribosomal protein S2
+Macromolecule #22: 30S ribosomal protein S1
+Macromolecule #23: 30S ribosomal protein S3
+Macromolecule #24: 30S ribosomal protein S4
+Macromolecule #25: 30S ribosomal protein S5
+Macromolecule #26: 30S ribosomal protein S6
+Macromolecule #27: 30S ribosomal protein S7
+Macromolecule #28: 30S ribosomal protein S8
+Macromolecule #29: 30S ribosomal protein S9
+Macromolecule #30: 30S ribosomal protein S10
+Macromolecule #31: 30S ribosomal protein S11
+Macromolecule #32: 30S ribosomal protein S12
+Macromolecule #33: 30S ribosomal protein S14
+Macromolecule #34: 30S ribosomal protein S15
+Macromolecule #35: 30S ribosomal protein S16
+Macromolecule #36: 30S ribosomal protein S17
+Macromolecule #37: 30S ribosomal protein S19
+Macromolecule #38: 30S ribosomal protein S13
+Macromolecule #39: 50S ribosomal protein L11
+Macromolecule #40: 50S ribosomal protein L7/L12
+Macromolecule #42: 50S ribosomal protein L27
+Macromolecule #43: 50S ribosomal protein L28
+Macromolecule #45: 50S ribosomal protein L29
+Macromolecule #46: 50S ribosomal protein L30
+Macromolecule #47: 50S ribosomal protein L31
+Macromolecule #48: 50S ribosomal protein L2
+Macromolecule #49: 50S ribosomal protein L32
+Macromolecule #50: 50S ribosomal protein L3
+Macromolecule #51: 50S ribosomal protein L33
+Macromolecule #52: 50S ribosomal protein L4
+Macromolecule #53: 50S ribosomal protein L34
+Macromolecule #54: 50S ribosomal protein L5
+Macromolecule #55: 50S ribosomal protein L35
+Macromolecule #56: 50S ribosomal protein L6
+Macromolecule #57: 50S ribosomal protein L36
+Macromolecule #58: 50S ribosomal protein L9
+Macromolecule #59: 50S ribosomal protein L13
+Macromolecule #60: 50S ribosomal protein L14
+Macromolecule #61: 50S ribosomal protein L15
+Macromolecule #62: 50S ribosomal protein L16
+Macromolecule #63: 50S ribosomal protein L17
+Macromolecule #64: 50S ribosomal protein L18
+Macromolecule #65: 50S ribosomal protein L19
+Macromolecule #66: 50S ribosomal protein L20
+Macromolecule #6: NT DNA ops
+Macromolecule #7: T DNA ops
+Macromolecule #8: mRNA with 24 nt long spacer
+Macromolecule #10: E-site and P-site fMet-tRNA
+Macromolecule #18: 16S rRNA
+Macromolecule #41: 23S rRNA
+Macromolecule #44: 5S rRNA
+Macromolecule #67: MAGNESIUM ION
+Macromolecule #68: ZINC ION
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TALOS ARCTICA |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 28.0 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.25 µm |
Experimental equipment | ![]() Model: Talos Arctica / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: NONE |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.4 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 14614 |
Initial angle assignment | Type: COMMON LINE |
Final angle assignment | Type: COMMON LINE |