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- PDB-8ury: Escherichia coli transcription-translation coupled complex class ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8ury | ||||||
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Title | Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, NusA, mRNA with a 30 nt long spacer, and fMet-tRNA in E-site and P-site of the ribosome | ||||||
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![]() | RIBOSOME / transcription / translation / coupling / expressome / NusA / RfaH / RNA / RNA polymerase | ||||||
Function / homology | ![]() transcription antitermination factor activity, DNA binding / transcription elongation-coupled chromatin remodeling / RNA polymerase complex / submerged biofilm formation / cellular response to cell envelope stress / cytosolic DNA-directed RNA polymerase complex / stringent response / regulation of DNA-templated transcription initiation / bacterial-type flagellum assembly / transcription antitermination factor activity, RNA binding ...transcription antitermination factor activity, DNA binding / transcription elongation-coupled chromatin remodeling / RNA polymerase complex / submerged biofilm formation / cellular response to cell envelope stress / cytosolic DNA-directed RNA polymerase complex / stringent response / regulation of DNA-templated transcription initiation / bacterial-type flagellum assembly / transcription antitermination factor activity, RNA binding / misfolded RNA binding / Group I intron splicing / RNA folding / bacterial-type flagellum-dependent cell motility / nitrate assimilation / translational termination / DnaA-L2 complex / negative regulation of translational initiation / negative regulation of DNA-templated DNA replication initiation / ribosome assembly / mRNA regulatory element binding translation repressor activity / positive regulation of RNA splicing / assembly of large subunit precursor of preribosome / transcription elongation factor complex / regulation of DNA-templated transcription elongation / cytosolic ribosome assembly / transcription antitermination / DNA-templated transcription initiation / cell motility / DNA-templated transcription termination / maintenance of translational fidelity / response to radiation / ribosomal large subunit assembly / ribonucleoside binding / mRNA 5'-UTR binding / DNA-directed 5'-3' RNA polymerase activity / DNA-directed RNA polymerase / large ribosomal subunit / ribosome biogenesis / ribosome binding / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / transferase activity / 5S rRNA binding / response to heat / large ribosomal subunit rRNA binding / protein-containing complex assembly / cytosolic small ribosomal subunit / intracellular iron ion homeostasis / cytosolic large ribosomal subunit / tRNA binding / cytoplasmic translation / protein dimerization activity / rRNA binding / negative regulation of translation / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / DNA-binding transcription factor activity / response to antibiotic / nucleotide binding / mRNA binding / magnesium ion binding / DNA binding / RNA binding / zinc ion binding / membrane / metal ion binding / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() synthetic construct (others) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.1 Å | ||||||
![]() | Molodtsov, V. / Wang, C. / Ebright, R.H. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structural basis of RfaH-mediated transcription-translation coupling Authors: Molodtsov, V. / Wang, C. / Zhang, J. / Kaelber, J.T. / Blaha, G. / Ebright, R.H. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 5.9 MB | Display | ![]() |
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PDB format | ![]() | Display | ![]() | |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 1.7 MB | Display | ![]() |
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Full document | ![]() | 1.9 MB | Display | |
Data in XML | ![]() | 304.6 KB | Display | |
Data in CIF | ![]() | 524.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 42504MC M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Assembly
Deposited unit | ![]()
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Components
+50S ribosomal protein ... , 25 types, 25 molecules 01239YZcfhijklmnpqrtuvwxz
-Large ribosomal subunit protein ... , 7 types, 7 molecules 4begosy
#5: Protein | Mass: 10713.465 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#42: Protein | Mass: 9146.540 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
#45: Protein | Mass: 7155.267 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
#47: Protein | Mass: 7887.117 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
#55: Protein | Mass: 7181.835 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
#59: Protein | Mass: 16050.606 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
#65: Protein | Mass: 13159.278 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
-DNA chain , 2 types, 2 molecules 56
#6: DNA chain | Mass: 11786.552 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others) / Production host: synthetic construct (others) |
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#7: DNA chain | Mass: 11534.374 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others) / Production host: synthetic construct (others) |
-RNA chain , 5 types, 6 molecules 7ABDad
#8: RNA chain | Mass: 14749.310 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others) / Production host: synthetic construct (others) | ||||||
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#10: RNA chain | Mass: 24496.617 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #18: RNA chain | | Mass: 499690.031 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #41: RNA chain | | Mass: 941635.438 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #44: RNA chain | | Mass: 38790.090 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
-DNA-directed RNA polymerase subunit ... , 4 types, 5 molecules AAACADAEAF
#11: Protein | Mass: 150820.875 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: rpoB, groN, nitB, rif, ron, stl, stv, tabD, b3987, JW3950 Production host: ![]() ![]() | ||||
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#13: Protein | Mass: 36558.680 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #14: Protein | | Mass: 155366.781 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #15: Protein | | Mass: 10249.547 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
-Transcription ... , 2 types, 2 molecules ABAG
#12: Protein | Mass: 18364.217 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#16: Protein | Mass: 54932.684 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
-30S ribosomal protein ... , 19 types, 19 molecules CEFGIJKLMNOQRSTUVWX
#17: Protein | Mass: 9005.472 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#19: Protein | Mass: 9708.464 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
#20: Protein | Mass: 8524.039 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
#21: Protein | Mass: 26781.670 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
#23: Protein | Mass: 26031.316 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
#24: Protein | Mass: 23514.199 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
#25: Protein | Mass: 17629.398 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
#26: Protein | Mass: 12125.993 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
#27: Protein | Mass: 20055.156 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
#28: Protein | Mass: 14146.557 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: rpsH, A5U30_004540, A8502_004035, A9X72_02245, ACN81_25460, ACU57_21020, AM464_09160, AW118_24840, AWP47_12705, B6R15_004081, B6R31_004980, BANRA_04316, BCB93_004281, BER14_25130, BF481_004942, ...Gene: rpsH, A5U30_004540, A8502_004035, A9X72_02245, ACN81_25460, ACU57_21020, AM464_09160, AW118_24840, AWP47_12705, B6R15_004081, B6R31_004980, BANRA_04316, BCB93_004281, BER14_25130, BF481_004942, BG944_001121, BGM66_003605, BJI68_08735, BJJ90_02185, BK292_20445, BK383_24705, BKL28_004555, BTB68_004368, BTQ06_10630, BvCmsKKP061_03018, BvCmsSIP010_02673, BXT93_05535, C0P57_002656, C1Q91_004916, C2121_004174, C2M16_25465, C2R31_004621, C3F40_19780, C9E67_02785, C9Z68_22575, CA593_09710, CF22_004861, CG692_20245, CG704_20930, CG831_003637, CIG67_10735, CQ986_004893, CR538_02480, CR539_21840, CR628_001354, CTR35_003618, CV83915_02792, CY655_21260, D1912_07455, D3G36_23110, D4M65_20595, D4N09_20195, D9D43_22610, D9E49_05180, D9J61_16675, DD762_23570, DIV22_15235, DNQ45_03780, DNX30_25455, DQ87_004788, DS732_24285, DTL43_20975, DU321_12025, E4K51_21515, E5H86_24500, E6D34_21735, EAI46_07500, EC95NR1_02697, ECs4171, EEP55_004504, EIA08_23980, EIMP300_17820, EIZ93_12445, EN85_004361, EPS76_07190, EPS97_20070, EWK56_25020, ExPECSC038_03652, F7F11_22700, F7N46_23985, F9407_19270, F9413_21210, F9461_25630, F9B07_24660, FEJ01_22490, FIJ20_21170, FJQ40_18330, FKO60_25660, FOI11_019235, FOI11_03945, FPI65_20330, FPS11_25405, FTV92_20690, FV293_22440, FVB16_04895, FWK02_06295, FZN31_02610, FZU14_21845, G3V95_19870, G3W53_20980, G4A38_21535, G4A47_20805, G5603_24610, G8C01_22530, G9448_17050, GAI89_24805, GAJ12_24695, GGB84_004463, GJ11_21410, GKF66_21570, GNW61_16655, GOP25_22730, GP711_23330, GP954_00920, GP965_04320, GP975_01210, GP979_01980, GQA06_03925, GQM04_10590, GQM13_25225, GQM21_11255, GQN34_23290, GQW07_21525, GRO95_20790, GRW05_09085, GRW24_04730, GRW56_00320, GRW57_03515, GTP92_23170, GUC01_21205, H0O72_19440, HCQ42_003944, HEP30_018660, HEP34_004766, HHH44_004531, HI055_004144, HIE29_005191, HJQ60_005007, HLX92_10050, HLZ50_22155, HMV95_19630, HV109_02165, HV146_21695, HV209_14690, HVV39_09290, HVW04_17510, HVW43_18645, HVY77_02150, HVZ29_19120, HX086_19430, HX136_02170, I0E88_004513, I4I37_25280, I6H00_20900, I6H02_12015, IFB95_004810, IUC12_004856, J0541_004401, J5U05_004083, JNP96_25330, KV259_004354, KV317_004326, KV371_004340, KV406_004333, KV449_004368, KV455_004331, KV463_004353, KV469_004346, KV499_004417, KV500_004304, NCTC10082_02835, NCTC10089_00498, NCTC10090_03523, NCTC10764_03859, NCTC10767_01512, NCTC11112_01040, NCTC11124_04717, NCTC11126_02767, NCTC11181_02734, NCTC11341_01893, NCTC12950_00475, NCTC13148_03445, NCTC4450_02471, NCTC7922_04046, NCTC7927_00547, NCTC7928_02520, NCTC8009_01507, NCTC8179_05938, NCTC8333_00485, NCTC8500_00329, NCTC8621_00504, NCTC8959_03265, NCTC8960_03066, NCTC8985_04978, NCTC9001_04250, NCTC9045_00561, NCTC9077_00600, NCTC9081_00913, NCTC9117_00752, NCTC9702_00533, NCTC9706_02733, NCTC9962_06793, NEP60_26315, O5851_12305, RG28_21635, SAMEA3752557_01956, TUM18780_04320, WR15_19630 Production host: ![]() ![]() |
#29: Protein | Mass: 14886.270 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: rpsI, A5U30_002029, A6592_14035, A8499_002400, A8502_003025, A9X72_02640, AC065_15530, ACN81_18270, ACU57_14275, AM464_08780, APX88_13640, AW118_08655, AWP47_10935, B6R12_000313, B6R15_000990, ...Gene: rpsI, A5U30_002029, A6592_14035, A8499_002400, A8502_003025, A9X72_02640, AC065_15530, ACN81_18270, ACU57_14275, AM464_08780, APX88_13640, AW118_08655, AWP47_10935, B6R12_000313, B6R15_000990, B6R17_003098, B6R31_001806, B6R48_002633, B6R87_002413, BANRA_04240, BB540_06935, BER14_09340, BF481_001923, BG944_002163, BGM66_003544, BHS81_19395, BJI68_19125, BJJ90_02580, BK292_10050, BK383_16130, BKL28_000276, BMT50_12670, BMT91_11440, BR158_000189, BTB68_000438, BTQ06_23470, BUO55_000295, BvCmsKKP061_00922, BvCmsSIP010_02602, BXT93_24385, BZL69_07895, C0P57_001852, C1Q91_000916, C2121_004467, C2M16_04035, C2R31_003461, C3F40_20175, C5N07_22725, C9E67_03180, C9Z68_06055, CA593_10120, CCS08_10250, CCU84_001112, CDL36_04425, CF22_002985, CG692_13375, CG704_16430, CG831_001247, CIG67_16815, CQ986_001737, CQB02_14740, CR538_02875, CR539_21445, CR628_001957, CRM83_23300, CTR35_000744, CV83915_02871, CWS33_08570, CX938_003362, CY655_20865, D1912_21090, D3C88_18900, D3G36_08955, D4M65_13015, D4N09_15115, D6T60_21260, D9D43_03785, D9E34_11910, D9E49_09750, D9H94_03690, D9J03_05350, D9J61_01070, DD762_12930, DIV22_02780, DNQ45_08935, DNX30_11580, DS732_23890, DTL43_04485, DTL90_07760, DTM45_14705, DU321_08840, E0I52_08435, E4K51_20805, E5H86_10565, E6D34_05915, EA239_09010, EAI46_01110, EB534_RS02840, EC95NR1_02610, ECs4103, EIA08_12335, EIZ93_10150, EN85_002333, EPS76_12290, EPS97_17880, ERS085406_02965, ERS139208_02274, EWK56_02620, ExPECSC038_02741, F7F11_08870, F7N46_13330, F9413_10425, F9461_07600, F9B07_01435, FA868_08430, FEJ01_06675, FFF58_13605, FGG80_03865, FHD44_17870, FHQ97_04375, FIJ20_03860, FJQ40_07185, FKO60_06410, FN993_000631, FOI11_019605, FOI11_03575, FPI65_19955, FPS11_10475, FTV92_21060, FTV93_21255, FV293_08185, FVB16_17315, FZN31_03025, FZU14_16365, G3V95_08965, G3W53_05220, G4A38_08495, G4A47_07885, G5603_16210, G9448_17420, GAI89_03645, GAJ12_14530, GAJ26_01560, GGB84_000282, GIB53_11705, GJ11_20975, GKF52_04875, GKF66_06285, GKF89_22220, GNW61_12520, GOP14_001750, GOP25_03570, GP711_11340, GP965_13255, GP975_23890, GP979_14590, GQA06_14425, GQM04_13525, GQM13_09620, GQM17_12695, GQM21_01635, GQN34_18070, GQW07_04110, GRO95_03860, GRW05_16510, GRW24_10515, GRW56_09935, GRW57_10250, GSM54_11730, GSY44_07995, GUC01_09885, GUI33_02630, H0O53_08235, H0O72_09800, H0P11_01640, H5Z35_000289, H6Y26_003879, HCQ42_000364, HEP30_003470, HEP34_000253, HHH44_002822, HI055_000385, HIE29_003186, HJQ60_003835, HJY54_000681, HKA49_001576, HLQ92_10840, HLV18_15680, HLX92_02455, HLZ50_05640, HMV95_21920, HMW38_00255, HV109_02535, HV146_21315, HV209_15060, HVV39_08880, HVW04_17925, HVW43_19020, HVY77_02530, HVZ29_18700, HVZ30_02410, HVZ71_02705, HX136_02545, I6H00_21285, I6H02_11635, IFB95_002590, IUD45_003198, J0541_003264, J4S20_002626, J5U05_001240, J7T50_001183, JFD_02469, JFD_04155, JNP96_24960, KQ660_11425, NCTC10082_02912, NCTC10089_00573, NCTC10090_03603, NCTC10418_00792, NCTC10487_05043, NCTC10764_03943, NCTC10767_01601, NCTC10865_00738, NCTC10974_00628, NCTC11112_00948, NCTC11126_02865, NCTC11127_03151, NCTC11181_02813, NCTC11341_01809, NCTC12950_00560, NCTC13127_00868, NCTC4450_02548, NCTC7922_03943, NCTC7927_00632, NCTC7928_02411, NCTC8179_06030, NCTC8450_05022, NCTC8500_00425, NCTC8621_00591, NCTC8622_00406, NCTC8959_03190, NCTC8960_03154, NCTC9001_05559, NCTC9044_01272, NCTC9045_00641, NCTC9050_03684, NCTC9071_00556, NCTC9073_00293, NCTC9075_00824, NCTC9077_00687, NCTC9081_01013, NCTC9111_00940, NCTC9117_00832, NCTC9702_00613, NCTC9706_02809, NCTC9775_04087, NCTC9777_01975, NCTC9962_00338, NEP60_07135, OS110_19195, RG28_21240, SAMEA3472044_04260, SAMEA3472056_03130, SAMEA3752557_01816, SAMEA3753106_02430, TUM18780_04980, WR15_24270 Production host: ![]() ![]() |
#31: Protein | Mass: 12487.200 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
#32: Protein | Mass: 13768.157 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
#33: Protein | Mass: 11606.560 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
#34: Protein | Mass: 10290.816 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
#35: Protein | Mass: 9207.572 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
#36: Protein | Mass: 9724.491 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: rpsQ, A5U30_004545, A6592_25760, A8499_003325, A8502_004030, A9X72_02220, AC065_15970, ACN81_25485, ACU57_20995, AM464_09185, APX88_16110, AW118_24865, AWP47_12680, B6R12_002814, B6R15_004076, ...Gene: rpsQ, A5U30_004545, A6592_25760, A8499_003325, A8502_004030, A9X72_02220, AC065_15970, ACN81_25485, ACU57_20995, AM464_09185, APX88_16110, AW118_24865, AWP47_12680, B6R12_002814, B6R15_004076, B6R17_004949, B6R31_004975, B6R48_005042, B6R87_004952, BANRA_04321, BB540_01705, BER14_25155, BF481_004937, BG944_001126, BGM66_003600, BHS81_19800, BJI68_08710, BJJ90_02160, BK292_20470, BK383_24680, BKL28_004550, BMT50_12250, BMT91_20035, BR158_003783, BTB68_004363, BTQ06_10655, BUO55_004704, BvCmsKKP061_03023, BvCmsSIP010_02678, BXT93_05510, BZL69_25995, C0P57_002661, C1Q91_004911, C2121_004169, C2M16_25440, C2R31_004626, C3F40_19755, C5N07_23525, C9E67_02760, C9Z68_22600, CA593_09685, CCS08_00180, CCU84_002909, CDL36_25545, CF22_004856, CG692_20220, CG704_20905, CG831_003642, CIG67_10710, CQ986_004888, CQB02_00675, CR538_02455, CR539_21865, CR628_001359, CRM83_22880, CTR35_003623, CV83915_02787, CWS33_24905, CX938_003824, CY655_21285, D1912_22345, D3C88_12855, D3G36_23085, D4M65_20620, D4N09_20220, D6T60_05740, D9D43_22585, D9E34_23560, D9E49_05205, D9H94_20785, D9J03_20100, D9J61_16700, DD762_23595, DIV22_15210, DNQ45_03805, DNX30_25430, DS732_24310, DTL43_20950, DTL90_24740, DTM45_24505, DU321_12050, E0I52_20190, E4K51_21540, E5H86_24475, E6D34_21710, EA239_22840, EAI46_07525, EB534_RS01750, EC95NR1_02702, ECs4176, EIA08_24005, EIZ93_12420, EN85_004356, EPS76_07165, EPS97_20045, ERS085406_04397, ERS139208_04345, EWK56_25045, ExPECSC038_03647, F3P10_05520, F7F11_22675, F7N46_23960, F9413_21185, F9461_25605, F9B07_24635, FA868_22795, FEJ01_22465, FFF58_25405, FGG80_25085, FHD44_21315, FHQ97_20070, FIJ20_21145, FJQ40_18355, FKO60_25685, FN993_004702, FOI11_019210, FOI11_03970, FPI65_20355, FPS11_25380, FTV92_20665, FTV93_20850, FV293_22415, FVB16_04920, FWK02_06320, FZN31_02585, FZU14_21870, G3V95_19895, G3W53_21005, G4A38_21560, G4A47_20830, G5603_24635, G9448_17025, GAI89_24780, GAJ12_24670, GAJ26_22060, GGB84_004458, GIB53_19350, GJ11_21435, GKF52_21290, GKF66_21595, GKF89_18520, GNW61_16680, GOP14_004282, GOP25_22755, GP711_23355, GP954_00895, GP965_04345, GP975_01235, GP979_01955, GQA06_03900, GQM04_10615, GQM13_25250, GQM17_19965, GQM21_11280, GQN34_23315, GQW07_21500, GRO95_20765, GRW05_09110, GRW24_04705, GRW56_00345, GRW57_03490, GSM54_23280, GSY44_20050, GUC01_21230, GUI33_20095, H0O53_20780, H0O72_19465, H0P11_20875, H5Z35_004780, H6Y26_003173, HCQ42_003939, HEP30_018685, HEP34_004761, HHH44_004526, HI055_004149, HIE29_005196, HJQ60_005002, HJY54_004744, HKA49_005026, HLQ92_22550, HLV18_23290, HLX92_10025, HLZ50_22130, HMV95_19655, HMW38_23155, HV109_02140, HV146_21720, HV209_14665, HVV39_09315, HVW04_17485, HVW43_18620, HVY77_02125, HVZ29_19145, HVZ30_02005, HVZ71_02310, HX136_02145, I6H00_20875, I6H02_12040, IFB95_004815, IUD45_003549, J0541_004406, J4S20_004793, J5U05_004078, J7T50_001462, JFD_01893, JFD_03722, JNP96_25355, KQ660_11820, NCTC10082_02830, NCTC10089_00493, NCTC10090_03518, NCTC10418_00682, NCTC10429_01085, NCTC10487_00436, NCTC10764_03854, NCTC10767_01507, NCTC10865_00650, NCTC10974_00540, NCTC11112_01045, NCTC11127_03232, NCTC11181_02729, NCTC11341_01898, NCTC12950_00470, NCTC13127_00777, NCTC4450_02466, NCTC7922_04051, NCTC7927_00542, NCTC7928_02526, NCTC8009_01502, NCTC8179_05933, NCTC8333_00480, NCTC8450_04928, NCTC8500_00325, NCTC8621_00499, NCTC8622_00515, NCTC8959_03270, NCTC8960_03061, NCTC8985_04973, NCTC9044_01367, NCTC9045_00556, NCTC9050_03602, NCTC9071_00474, NCTC9073_00388, NCTC9075_00715, NCTC9077_00595, NCTC9081_00908, NCTC9111_00858, NCTC9117_00747, NCTC9702_00528, NCTC9706_02728, NCTC9775_03994, NCTC9777_01882, NCTC9962_06788, NEP60_26340, OS110_25165, RG28_21660, SAMEA3472044_01153, SAMEA3472056_05420, SAMEA3752557_01961, SAMEA3753106_04625, TUM18780_04270, WR15_19655 Production host: ![]() ![]() |
#37: Protein | Mass: 10455.355 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: rpsS, rpsS_1, A5U30_004550, A8502_004025, A9X72_02195, ACN81_25510, ACU57_20970, AM464_09210, AW118_24890, AWP47_12655, B6R15_004071, B6R31_004970, BANRA_04326, BCB93_004291, BER14_25180, BF481_ ...Gene: rpsS, rpsS_1, A5U30_004550, A8502_004025, A9X72_02195, ACN81_25510, ACU57_20970, AM464_09210, AW118_24890, AWP47_12655, B6R15_004071, B6R31_004970, BANRA_04326, BCB93_004291, BER14_25180, BF481_004932, BG944_001131, BGM66_003595, BJI68_08685, BJJ90_02135, BK292_20495, BK383_24655, BKL28_004545, BTB68_004358, BTQ06_10680, BvCmsKKP061_03028, BvCmsSIP010_02683, BXT93_05485, C0P57_002666, C1Q91_004906, C2121_004164, C2M16_25415, C2R31_004631, C3F40_19730, C9E67_02735, C9Z68_22625, CA593_09660, CF22_004851, CG692_20195, CG704_20880, CG831_003647, CIG67_10685, CQ986_004883, CR538_02430, CR539_21890, CR628_001364, CTR35_003628, CY655_21310, D1912_22370, D3G36_23060, D4M65_20645, D4N09_20245, D9D43_22560, D9E49_05230, D9J61_16725, DD762_23620, DIV22_15185, DNQ45_03830, DNX30_25405, DQ87_004778, DTL43_20925, DU321_12075, E4K51_21565, E5H86_24450, E6D34_21685, EAI46_07550, EC95NR1_02707, ECs4181, EEP55_004494, EIA08_24030, EIZ93_12395, EN85_004351, EPS76_07140, EPS97_20020, EWK56_25070, ExPECSC038_03642, F7F11_22650, F7N46_23935, F9407_19220, F9413_21160, F9461_25580, F9B07_24610, FEJ01_22440, FIJ20_21120, FJQ40_18380, FKO60_25710, FOI11_019185, FOI11_03995, FPI65_20380, FPS11_25355, FTV92_20640, FTV93_20825, FV293_22390, FVB16_04945, FWK02_12640, FZN31_02560, FZU14_21895, G3V95_19920, G3W53_21030, G4A38_21585, G4A47_20855, G5603_24660, G8C01_22480, G9448_17000, GAI89_24755, GAJ12_24645, GGB84_004453, GJ11_21460, GKF66_21620, GNW61_16705, GOP25_22780, GP711_23380, GP954_00870, GP965_04370, GP975_01260, GP979_01930, GQA06_03875, GQM04_10640, GQM13_25275, GQM21_11305, GQN34_23340, GQW07_21475, GRO95_20740, GRW05_09135, GRW24_04680, GRW56_00370, GRW57_03465, GTP92_23120, GUC01_21255, H0O72_19490, HCQ42_003934, HEP30_018710, HEP34_004756, HHH44_004521, HI055_004154, HIE29_005201, HJQ60_004997, HLX92_10000, HLZ50_22105, HMV95_19680, HV109_02115, HV146_21745, HV209_14640, HVV39_09340, HVW04_17460, HVW43_18595, HVY77_02100, HVZ29_19170, HX086_19480, HX136_02120, I0E88_004503, I4I37_21030, I6H00_20850, I6H02_12065, IFB95_004820, IUC12_004846, J0541_004411, J5U05_004073, JNP96_25380, KV259_004344, KV317_004316, KV371_004330, KV406_004323, KV449_004358, KV455_004321, KV463_004343, KV469_004336, KV499_004407, KV500_004294, NCTC10082_02825, NCTC10089_00488, NCTC10090_03513, NCTC10429_01079, NCTC10764_03849, NCTC10767_01502, NCTC10865_00644, NCTC10974_00535, NCTC11112_01049, NCTC11126_02756, NCTC11181_02724, NCTC11341_01903, NCTC12950_00465, NCTC4450_02461, NCTC7927_00537, NCTC7928_02531, NCTC8179_05927, NCTC8333_00475, NCTC8500_00320, NCTC8621_00494, NCTC8622_00520, NCTC8959_03275, NCTC8960_03056, NCTC8985_04967, NCTC9001_04240, NCTC9044_01374, NCTC9045_00551, NCTC9077_00590, NCTC9081_00903, NCTC9117_00742, NCTC9702_00523, NCTC9706_02723, NCTC9962_06783, NEP60_26365, O5851_12255, RG28_21685, SAMEA3752557_01966, TUM18780_04220, WR15_19680 Production host: ![]() ![]() |
#38: Protein | Mass: 13128.467 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
-Small ribosomal subunit protein ... , 2 types, 2 molecules HP
#22: Protein | Mass: 61238.887 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#30: Protein | Mass: 11254.041 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
-Non-polymers , 2 types, 3 molecules ![](data/chem/img/MG.gif)
![](data/chem/img/ZN.gif)
![](data/chem/img/ZN.gif)
#67: Chemical | ChemComp-MG / |
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#68: Chemical |
-Details
Has ligand of interest | N |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
Component | Name: Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, NusA, mRNA with a 30 nt long spacer, and fMet-tRNA in E-site and P-site of the ribosome Type: COMPLEX / Entity ID: #1-#66 / Source: RECOMBINANT |
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Source (natural) | Organism: ![]() ![]() |
Source (recombinant) | Organism: ![]() ![]() |
Buffer solution | pH: 7.6 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2500 nm / Nominal defocus min: 800 nm |
Image recording | Electron dose: 45 e/Å2 / Film or detector model: GATAN K2 SUMMIT (4k x 4k) |
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Processing
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION |
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3D reconstruction | Resolution: 3.1 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 12096 / Symmetry type: POINT |