EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	A mechanistic model of primer synthesis from catalytic structures of DNA polymerase α-primase.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 31, Issue 5, Page 777-790, Year 2024
掲載日	2024年3月15日
著者	Elwood A Mullins / Lauren E Salay / Clarissa L Durie / Noah P Bradley / Jane E Jackman / Melanie D Ohi / Walter J Chazin / Brandt F Eichman /
PubMed 要旨	The mechanism by which polymerase α-primase (polα-primase) synthesizes chimeric RNA-DNA primers of defined length and composition, necessary for replication fidelity and genome stability, is ...The mechanism by which polymerase α-primase (polα-primase) synthesizes chimeric RNA-DNA primers of defined length and composition, necessary for replication fidelity and genome stability, is unknown. Here, we report cryo-EM structures of Xenopus laevis polα-primase in complex with primed templates representing various stages of DNA synthesis. Our data show how interaction of the primase regulatory subunit with the primer 5' end facilitates handoff of the primer to polα and increases polα processivity, thereby regulating both RNA and DNA composition. The structures detail how flexibility within the heterotetramer enables synthesis across two active sites and provide evidence that termination of DNA synthesis is facilitated by reduction of polα and primase affinities for the varied conformations along the chimeric primer-template duplex. Together, these findings elucidate a critical catalytic step in replication initiation and provide a comprehensive model for primer synthesis by polα-primase.
リンク	Nat Struct Mol Biol / PubMed:38491139 / PubMed Central
手法	EM (単粒子)
解像度	2.8 - 14.06 Å
構造データ	29862 8g99 EMDB-29862, PDB-8g99: Partial auto-inhibitory complex of Xenopus laevis DNA polymerase alpha-primase 手法: EM (単粒子) / 解像度: 2.8 Å 29864 8g9f EMDB-29864, PDB-8g9f: Complete auto-inhibitory complex of Xenopus laevis DNA polymerase alpha-primase 手法: EM (単粒子) / 解像度: 3.2 Å 29871 8g9l EMDB-29871, PDB-8g9l: DNA initiation subcomplex of Xenopus laevis DNA polymerase alpha-primase 手法: EM (単粒子) / 解像度: 3.3 Å 29872 8g9n EMDB-29872, PDB-8g9n: Partial DNA elongation subcomplex of Xenopus laevis DNA polymerase alpha-primase 手法: EM (単粒子) / 解像度: 3.5 Å 29873 8g9o EMDB-29873, PDB-8g9o: Complete DNA elongation subcomplex of Xenopus laevis DNA polymerase alpha-primase 手法: EM (単粒子) / 解像度: 4.4 Å 29888 8v5m EMDB-29888, PDB-8v5m: Tetramer core subcomplex (conformation 1) of Xenopus laevis DNA polymerase alpha-primase 手法: EM (単粒子) / 解像度: 5.3 Å 29889 8v5n EMDB-29889, PDB-8v5n: Tetramer core subcomplex (conformation 2) of Xenopus laevis DNA polymerase alpha-primase 手法: EM (単粒子) / 解像度: 4.2 Å 29891 8v5o EMDB-29891, PDB-8v5o: Tetramer core subcomplex (conformation 3) of Xenopus laevis DNA polymerase alpha-primase 手法: EM (単粒子) / 解像度: 4.7 Å 42140 8ucu EMDB-42140, PDB-8ucu: Partial DNA termination subcomplex of Xenopus laevis DNA polymerase alpha-primase 手法: EM (単粒子) / 解像度: 2.85 Å 42141 8ucv EMDB-42141, PDB-8ucv: Complete DNA termination subcomplex 1 of Xenopus laevis DNA polymerase alpha-primase 手法: EM (単粒子) / 解像度: 3.81 Å 42142 8ucw EMDB-42142, PDB-8ucw: Complete DNA termination subcomplex 2 of Xenopus laevis DNA polymerase alpha-primase 手法: EM (単粒子) / 解像度: 3.64 Å 42990 8v6g EMDB-42990, PDB-8v6g: DNA initiation complex (configuration 1) of Xenopus laevis DNA polymerase alpha-primase 手法: EM (単粒子) / 解像度: 11.16 Å 42991 8v6h EMDB-42991, PDB-8v6h: DNA initiation complex (configuration 2) of Xenopus laevis DNA polymerase alpha-primase 手法: EM (単粒子) / 解像度: 11.11 Å 42992 8v6i EMDB-42992, PDB-8v6i: DNA elongation complex (configuration 1) of Xenopus laevis DNA polymerase alpha-primase 手法: EM (単粒子) / 解像度: 14.06 Å 42993 8v6j EMDB-42993, PDB-8v6j: DNA elongation complex (configuration 2) of Xenopus laevis DNA polymerase alpha-primase 手法: EM (単粒子) / 解像度: 11.11 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-SF4: IRON/SULFUR CLUSTER ChemComp-DGT: 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE / dGTP ChemComp-MG: Unknown entry / マグネシウムジカチオン
由来	xenopus laevis (アフリカツメガエル) synthetic construct (人工物)
キーワード	REPLICATION / TRANSFERASE / Primase / DNA polymerase / chimeric RNA-DNA primer / RNA/DNA hybrid / DNA replication / DNA synthesis / TRANSFERASE/DNA/RNA / TRANSFERASE-DNA-RNA complex