EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	P. aeruginosa CtpA protease adopts a novel activation mechanism to initiate the proteolytic process.
ジャーナル・号・ページ	EMBO J, Vol. 43, Issue 8, Page 1634-1652, Year 2024
掲載日	2024年3月11日
著者	Hao-Chi Hsu / Michelle Wang / Amanda Kovach / Andrew J Darwin / Huilin Li /
PubMed 要旨	During bacterial cell growth, hydrolases cleave peptide cross-links between strands of the peptidoglycan sacculus to allow new strand insertion. The Pseudomonas aeruginosa carboxyl-terminal ...During bacterial cell growth, hydrolases cleave peptide cross-links between strands of the peptidoglycan sacculus to allow new strand insertion. The Pseudomonas aeruginosa carboxyl-terminal processing protease (CTP) CtpA regulates some of these hydrolases by degrading them. CtpA assembles as an inactive hexamer composed of a trimer-of-dimers, but its lipoprotein binding partner LbcA activates CtpA by an unknown mechanism. Here, we report the cryo-EM structures of the CtpA-LbcA complex. LbcA has an N-terminal adaptor domain that binds to CtpA, and a C-terminal superhelical tetratricopeptide repeat domain. One LbcA molecule attaches to each of the three vertices of a CtpA hexamer. LbcA triggers relocation of the CtpA PDZ domain, remodeling of the substrate binding pocket, and realignment of the catalytic residues. Surprisingly, only one CtpA molecule in a CtpA dimer is activated upon LbcA binding. Also, a long loop from one CtpA dimer inserts into a neighboring dimer to facilitate the proteolytic activity. This work has revealed an activation mechanism for a bacterial CTP that is strikingly different from other CTPs that have been characterized structurally.
リンク	EMBO J / PubMed:38467832 / PubMed Central
手法	EM (単粒子)
解像度	3.55 - 4.14 Å
構造データ	EMDB-40846: The C-terminal protease CtpA-LbcA complex of pseudomonas aeruginosa with the TPR at the low position 手法: EM (単粒子) / 解像度: 4.14 Å EMDB-40847: The C-terminal protease CtpA-LbcA complex of pseudomonas aeruginosa with the TPR at the high position 手法: EM (単粒子) / 解像度: 4.04 Å EMDB-40848: The structure of C-terminal protease CtpA-LbcA complex of Pseudomonas aeruginosa 手法: EM (単粒子) / 解像度: 3.84 Å 40849 8sxe EMDB-40849, PDB-8sxe: Structure of the C-terminal protease CtpA-LbcA complex of Pseudomonas aeruginosa 手法: EM (単粒子) / 解像度: 3.55 Å 40850 8sxf EMDB-40850, PDB-8sxf: The C-terminal protease CtpA-LbcA complex of pseudomonas aeruginosa with the TPR at the high position 手法: EM (単粒子) / 解像度: 3.92 Å 40851 8sxg EMDB-40851, PDB-8sxg: The C-terminal protease CtpA-LbcA complex of pseudomonas aeruginosa with the TPR at the low position 手法: EM (単粒子) / 解像度: 4.14 Å 40852 8sxh EMDB-40852, PDB-8sxh: Structure of the C-terminal protease CtpA-LbcA complex of Pseudomonas aeruginosa 手法: EM (単粒子) / 解像度: 3.94 Å
由来	pseudomonas aeruginosa (緑膿菌) Escherichia coli (大腸菌) escherichia coli bl21(de3) (大腸菌)
キーワード	HYDROLASE / CtpA / LbcA / C-terminal protease / pseudomonas aeruginosa