EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure determination by cryoEM at 100 keV.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 120, Issue 49, Page e2312905120, Year 2023
掲載日	2023年12月5日
著者	Greg McMullan / Katerina Naydenova / Daniel Mihaylov / Keitaro Yamashita / Mathew J Peet / Hugh Wilson / Joshua L Dickerson / Shaoxia Chen / Giuseppe Cannone / Yang Lee / Katherine A Hutchings / Olivia Gittins / Mohamed A Sobhy / Torquil Wells / Mohamed M El-Gomati / Jason Dalby / Matthias Meffert / Clemens Schulze-Briese / Richard Henderson / Christopher J Russo /
PubMed 要旨	Electron cryomicroscopy can, in principle, determine the structures of most biological molecules but is currently limited by access, specimen preparation difficulties, and cost. We describe a purpose- ...Electron cryomicroscopy can, in principle, determine the structures of most biological molecules but is currently limited by access, specimen preparation difficulties, and cost. We describe a purpose-built instrument operating at 100 keV-including advances in electron optics, detection, and processing-that makes structure determination fast and simple at a fraction of current costs. The instrument attains its theoretical performance limits, allowing atomic resolution imaging of gold test specimens and biological molecular structure determination in hours. We demonstrate its capabilities by determining the structures of eleven different specimens, ranging in size from 140 kDa to 2 MDa, using a fraction of the data normally required. CryoEM with a microscope designed specifically for high-efficiency, on-the-spot imaging of biological molecules will expand structural biology to a wide range of previously intractable problems.
リンク	Proc Natl Acad Sci U S A / PubMed:38011573 / PubMed Central
手法	EM (単粒子)
解像度	2.6 - 4.5 Å
構造データ	17958 8pv9 EMDB-17958, PDB-8pv9: Structure of DPS determined by cryoEM at 100 keV 手法: EM (単粒子) / 解像度: 2.7 Å 17959 8pva EMDB-17959, PDB-8pva: Structure of bacterial ribosome determined by cryoEM at 100 keV 手法: EM (単粒子) / 解像度: 4.5 Å 17960 8pvb EMDB-17960, PDB-8pvb: Structure of GABAAR determined by cryoEM at 100 keV 手法: EM (単粒子) / 解像度: 3.6 Å 17961 8pvc EMDB-17961, PDB-8pvc: Structure of mouse heavy-chain apoferritin determined by cryoEM at 100 keV 手法: EM (単粒子) / 解像度: 2.6 Å 17962 8pvd EMDB-17962, PDB-8pvd: Structure of catalase determined by cryoEM at 100 keV 手法: EM (単粒子) / 解像度: 3.4 Å 17963 8pve EMDB-17963, PDB-8pve: Structure of AHIR determined by cryoEM at 100 keV 手法: EM (単粒子) / 解像度: 3.3 Å 17964 8pvf EMDB-17964, PDB-8pvf: Structure of GAPDH determined by cryoEM at 100 keV 手法: EM (単粒子) / 解像度: 2.9 Å 17965 8pvg EMDB-17965, PDB-8pvg: Structure of E. coli glutamine synthetase determined by cryoEM at 100 keV 手法: EM (単粒子) / 解像度: 3.4 Å 17966 8pvh EMDB-17966, PDB-8pvh: Structure of human apo ALDH1A1 determined by cryoEM at 100 keV 手法: EM (単粒子) / 解像度: 2.9 Å 17967 8pvi EMDB-17967, PDB-8pvi: Structure of PaaZ determined by cryoEM at 100 keV 手法: EM (単粒子) / 解像度: 3.7 Å 17968 8pvj EMDB-17968, PDB-8pvj: Structure of lumazine synthase determined by cryoEM at 100 keV 手法: EM (単粒子) / 解像度: 3.0 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-R16: HEXADECANE / ヘキサデカン ChemComp-D10: DECANE / デカン ChemComp-HSM: HISTAMINE / ヒスタミン / 神経伝達物質, ホルモンYM ChemComp-CL: Unknown entry / クロリド ChemComp-V8D: Etomidate / エトミダ-ト ChemComp-FE: Unknown entry ChemComp-NDP: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / NADPH ChemComp-HEM: PROTOPORPHYRIN IX CONTAINING FE ChemComp-NAD: NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NADYM
由来	escherichia coli (大腸菌) homo sapiens (ヒト) lama glama (ラマ) mus musculus (ハツカネズミ) thermus thermophilus (バクテリア) cryptosporidium parvum (小形クリプトスポリジウム) aquifex aeolicus (バクテリア)
キーワード	DNA BINDING PROTEIN / RIBOSOME / MEMBRANE PROTEIN / Pentameric ligand-gated ion channel / Neurotrasmitter receptor / GABA(A) receptor / METAL BINDING PROTEIN / Iron storage / OXIDOREDUCTASE / GAPDH / BIOSYNTHETIC PROTEIN / Glutamine synthetase / filament / Oxidoreductase Aldehyde dehydrogenase / HYDROLASE / substrate channeling / bi-functional enzyme / dehydrogenase / TRANSFERASE / LUMAZINE SYNTHASE