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-Structure paper
タイトル | Molecular recognition of trehalose and trehalose analogues by LpqY-SugABC. |
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ジャーナル・号・ページ | Proc Natl Acad Sci U S A, Vol. 120, Issue 35, Page e2307625120, Year 2023 |
掲載日 | 2023年8月29日 |
著者 | Jingxi Liang / Fengjiang Liu / Peng Xu / Wei Shangguan / Tianyu Hu / Shule Wang / Xiaolin Yang / Zhiqi Xiong / Xiuna Yang / Luke W Guddat / Biao Yu / Zihe Rao / Bing Zhang / |
PubMed 要旨 | Trehalose plays a crucial role in the survival and virulence of the deadly human pathogen (). The type I ATP-binding cassette (ABC) transporter LpqY-SugABC is the sole pathway for trehalose to enter ...Trehalose plays a crucial role in the survival and virulence of the deadly human pathogen (). The type I ATP-binding cassette (ABC) transporter LpqY-SugABC is the sole pathway for trehalose to enter . The substrate-binding protein, LpqY, which forms a stable complex with the translocator SugABC, recognizes and captures trehalose and its analogues in the periplasmic space, but the precise molecular mechanism for this process is still not well understood. This study reports a 3.02-Å cryoelectron microscopy structure of trehalose-bound LpqY-SugABC in the pretranslocation state, a crystal structure of LpqY in a closed form with trehalose bound and five crystal structures of LpqY in complex with different trehalose analogues. These structures, accompanied by substrate-stimulated ATPase activity data, reveal how LpqY recognizes and binds trehalose and its analogues, and highlight the flexibility in the substrate binding pocket of LpqY. These data provide critical insights into the design of trehalose analogues that could serve as potential molecular probe tools or as anti-TB drugs. |
リンク | Proc Natl Acad Sci U S A / PubMed:37603751 / PubMed Central |
手法 | EM (単粒子) / X線回折 |
解像度 | 1.6 - 3.02 Å |
構造データ | EMDB-36125, PDB-8ja7: PDB-8ja8: PDB-8ja9: PDB-8jaa: PDB-8jab: PDB-8jac: PDB-8jad: |
化合物 | ChemComp-SO4: ChemComp-HOH: ChemComp-TY6: ChemComp-U0X: ChemComp-W4Z: ChemComp-GLC: ChemComp-BEZ: |
由来 |
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キーワード | MEMBRANE PROTEIN / ABC transporter / SUGAR BINDING PROTEIN / Trehalose / LpqY / Mycobacterium tuberculosis |