EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Visualizing the nucleoplasmic maturation of human pre-60S ribosomal particles.
ジャーナル・号・ページ	Cell Res, Vol. 33, Issue 11, Page 867-878, Year 2023
掲載日	2023年7月25日
著者	Yunyang Zhang / Xiaomeng Liang / Sha Luo / Yan Chen / Yu Li / Chengying Ma / Ningning Li / Ning Gao /
PubMed 要旨	Eukaryotic ribosome assembly is a highly orchestrated process that involves over two hundred protein factors. After early assembly events on nascent rRNA in the nucleolus, pre-60S particles undergo ...Eukaryotic ribosome assembly is a highly orchestrated process that involves over two hundred protein factors. After early assembly events on nascent rRNA in the nucleolus, pre-60S particles undergo continuous maturation steps in the nucleoplasm, and prepare for nuclear export. Here, we report eleven cryo-EM structures of the nuclear pre-60S particles isolated from human cells through epitope-tagged GNL2, at resolutions of 2.8-4.3 Å. These high-resolution snapshots provide fine details for several major structural remodeling events at a virtual temporal resolution. Two new human nuclear factors, L10K and C11orf98, were also identified. Comparative structural analyses reveal that many assembly factors act as successive place holders to control the timing of factor association/dissociation events. They display multi-phasic binding properties for different domains and generate complex binding inter-dependencies as a means to guide the rRNA maturation process towards its mature conformation. Overall, our data reveal that nuclear assembly of human pre-60S particles is generally hierarchical with short branch pathways, and a few factors display specific roles as rRNA chaperones by confining rRNA helices locally to facilitate their folding, such as the C-terminal domain of SDAD1.
リンク	Cell Res / PubMed:37491604 / PubMed Central
手法	EM (単粒子)
解像度	2.8 - 4.3 Å
構造データ	35370 8idt EMDB-35370, PDB-8idt: human nuclear pre-60S ribosomal particle - State G 手法: EM (単粒子) / 解像度: 2.8 Å 35371 8idy EMDB-35371, PDB-8idy: human nuclear pre-60S ribosomal particle - State F 手法: EM (単粒子) / 解像度: 3.0 Å 35375 8ie3 EMDB-35375, PDB-8ie3: human nuclear pre-60S ribosomal particle - State E 手法: EM (単粒子) / 解像度: 3.3 Å 35596 8ine EMDB-35596, PDB-8ine: human nuclear pre-60S ribosomal particle - State G' 手法: EM (単粒子) / 解像度: 3.2 Å 35597 8inf EMDB-35597, PDB-8inf: human nuclear pre-60S ribosomal particle - State F' 手法: EM (単粒子) / 解像度: 3.0 Å 35599 8ink EMDB-35599, PDB-8ink: human nuclear pre-60S ribosomal particle - State D 手法: EM (単粒子) / 解像度: 3.2 Å 35639 8ipd EMDB-35639, PDB-8ipd: human nuclear pre-60S ribosomal particle - State C 手法: EM (単粒子) / 解像度: 3.2 Å 35649 8ipx EMDB-35649, PDB-8ipx: human nuclear pre-60S ribosomal particle - State C' 手法: EM (単粒子) / 解像度: 4.3 Å 35651 8ipy EMDB-35651, PDB-8ipy: human nuclear pre-60S ribosomal particle - State D' 手法: EM (単粒子) / 解像度: 3.2 Å 35672 8ir1 EMDB-35672, PDB-8ir1: human nuclear pre-60S ribosomal particle - State A 手法: EM (単粒子) / 解像度: 3.3 Å 35673 8ir3 EMDB-35673, PDB-8ir3: human nuclear pre-60S ribosomal particle - State B' 手法: EM (単粒子) / 解像度: 3.5 Å
化合物	ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-K: Unknown entry / カリウムカチオン ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM
由来	homo sapiens (ヒト)
キーワード	RIBOSOME / GNL2 / nuclear / pre-60S