EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of the human ATP synthase.
ジャーナル・号・ページ	Mol Cell, Vol. 83, Issue 12, Page 2137-22147.e4, Year 2023
掲載日	2023年6月15日
著者	Yuezheng Lai / Yuying Zhang / Shan Zhou / Jinxu Xu / Zhanqiang Du / Ziyan Feng / Long Yu / Ziqing Zhao / Weiwei Wang / Yanting Tang / Xiuna Yang / Luke W Guddat / Fengjiang Liu / Yan Gao / Zihe Rao / Hongri Gong /
PubMed 要旨	Biological energy currency ATP is produced by FF-ATP synthase. However, the molecular mechanism for human ATP synthase action remains unknown. Here, we present snapshot images for three main ...Biological energy currency ATP is produced by FF-ATP synthase. However, the molecular mechanism for human ATP synthase action remains unknown. Here, we present snapshot images for three main rotational states and one substate of human ATP synthase using cryoelectron microscopy. These structures reveal that the release of ADP occurs when the β subunit of FF-ATP synthase is in the open conformation, showing how ADP binding is coordinated during synthesis. The accommodation of the symmetry mismatch between F and F motors is resolved by the torsional flexing of the entire complex, especially the γ subunit, and the rotational substep of the c subunit. Water molecules are identified in the inlet and outlet half-channels, suggesting that the proton transfer in these two half-channels proceed via a Grotthus mechanism. Clinically relevant mutations are mapped to the structure, showing that they are mainly located at the subunit-subunit interfaces, thus causing instability of the complex.
リンク	Mol Cell / PubMed:37244256
手法	EM (単粒子)
解像度	2.53 - 8.0 Å
構造データ	34564 8h9e EMDB-34564, PDB-8h9e: Human ATP synthase F1 domain, state 1 手法: EM (単粒子) / 解像度: 2.53 Å 34565 8h9f EMDB-34565, PDB-8h9f: Human ATP synthase state 1 subregion 3 手法: EM (単粒子) / 解像度: 2.69 Å 34566 8h9g EMDB-34566, PDB-8h9g: Human ATP synthase state 1 subregion 2 手法: EM (単粒子) / 解像度: 2.95 Å 34568 8h9i EMDB-34568, PDB-8h9i: Human ATP synthase F1 domain, state2 手法: EM (単粒子) / 解像度: 2.77 Å 34569 8h9j EMDB-34569, PDB-8h9j: Human ATP synthase state2 subregion 3 手法: EM (単粒子) / 解像度: 3.26 Å 34570 8h9k EMDB-34570, PDB-8h9k: Human ATP synthase state 2 subregion 2 手法: EM (単粒子) / 解像度: 3.51 Å 34572 8h9l EMDB-34572, PDB-8h9l: Human ATP synthase F1 domain, state 3a 手法: EM (単粒子) / 解像度: 2.61 Å 34573 8h9m EMDB-34573, PDB-8h9m: Human ATP synthase state 3a subregion 3 手法: EM (単粒子) / 解像度: 3.0 Å 34574 8h9n EMDB-34574, PDB-8h9n: Human ATP synthase state 3a subregion 2 手法: EM (単粒子) / 解像度: 3.56 Å 34576 8h9p EMDB-34576, PDB-8h9p: Human ATP synthase F1 domain, state 3b 手法: EM (単粒子) / 解像度: 3.02 Å 34577 8h9q EMDB-34577, PDB-8h9q: Human ATP synthase state 3b subregion 3 手法: EM (単粒子) / 解像度: 3.47 Å 34578 8h9r EMDB-34578, PDB-8h9r: Human ATP synthase state 3b subregion 2 手法: EM (単粒子) / 解像度: 3.97 Å 34580 8h9s EMDB-34580, PDB-8h9s: Human ATP synthase state 1 (combined) 手法: EM (単粒子) / 解像度: 2.53 Å 34581 8h9t EMDB-34581, PDB-8h9t: Human ATP synthase state 2 (combined) 手法: EM (単粒子) / 解像度: 2.77 Å 34582 8h9u EMDB-34582, PDB-8h9u: Human ATP synthase state 3a (combined) 手法: EM (単粒子) / 解像度: 2.61 Å 34583 8h9v EMDB-34583, PDB-8h9v: Human ATP synthase state 3b (combined) 手法: EM (単粒子) / 解像度: 3.02 Å EMDB-34584: Human ATP synthase dimer 手法: EM (単粒子) / 解像度: 8.0 Å
化合物	ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-CDL: CARDIOLIPIN / リン脂質YM ChemComp-3PH*: 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE / ジステアロイルホスファチジン酸
由来	homo sapiens (ヒト)
キーワード	MEMBRANE PROTEIN / ATP synthase