EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	A robust approach for MicroED sample preparation of lipidic cubic phase embedded membrane protein crystals.
ジャーナル・号・ページ	Nat Commun, Vol. 14, Issue 1, Page 1086, Year 2023
掲載日	2023年2月25日
著者	Michael W Martynowycz / Anna Shiriaeva / Max T B Clabbers / William J Nicolas / Sara J Weaver / Johan Hattne / Tamir Gonen /
PubMed 要旨	Crystallizing G protein-coupled receptors (GPCRs) in lipidic cubic phase (LCP) often yields crystals suited for the cryogenic electron microscopy (cryoEM) method microcrystal electron diffraction ...Crystallizing G protein-coupled receptors (GPCRs) in lipidic cubic phase (LCP) often yields crystals suited for the cryogenic electron microscopy (cryoEM) method microcrystal electron diffraction (MicroED). However, sample preparation is challenging. Embedded crystals cannot be targeted topologically. Here, we use an integrated fluorescence light microscope (iFLM) inside of a focused ion beam and scanning electron microscope (FIB-SEM) to identify fluorescently labeled GPCR crystals. Crystals are targeted using the iFLM and LCP is milled using a plasma focused ion beam (pFIB). The optimal ion source for preparing biological lamellae is identified using standard crystals of proteinase K. Lamellae prepared using either argon or xenon produced the highest quality data and structures. MicroED data are collected from the milled lamellae and the structures are determined. This study outlines a robust approach to identify and mill membrane protein crystals for MicroED and demonstrates plasma ion-beam milling is a powerful tool for preparing biological lamellae.
リンク	Nat Commun / PubMed:36841804 / PubMed Central
手法	EM (電子線結晶学)
解像度	1.39 - 2.0 Å
構造データ	29586 8fyn EMDB-29586, PDB-8fyn: MicroED structure of A2A from plasma milled lamellae 手法: EM (電子線結晶学) / 解像度: 2.0 Å 29587 8fyo EMDB-29587, PDB-8fyo: MicroED structure of Proteinase K from lamellae milled from multiple plasma sources 手法: EM (電子線結晶学) / 解像度: 1.39 Å 29588 8fyp EMDB-29588, PDB-8fyp: MicroED structure of Proteinase K from xenon milled lamellae 手法: EM (電子線結晶学) / 解像度: 1.45 Å 29590 8fyq EMDB-29590, PDB-8fyq: MicroED structure of Proteinase K from argon milled lamellae 手法: EM (電子線結晶学) / 解像度: 1.4 Å 29595 8fyr EMDB-29595, PDB-8fyr: MicroED structure of Proteinase K from oxygen milled lamellae 手法: EM (電子線結晶学) / 解像度: 1.5 Å 29596 8fys EMDB-29596, PDB-8fys: MicroED structure of Proteinase K from nitrogen milled lamellae 手法: EM (電子線結晶学) / 解像度: 1.8 Å
化合物	ChemComp-ZMA: 4-{2-[(7-amino-2-furan-2-yl[1,2,4]triazolo[1,5-a][1,3,5]triazin-5-yl)amino]ethyl}phenol / ZM-241385 / アンタゴニストYM ChemComp-CLR: CHOLESTEROL / コレステロ-ル ChemComp-OLA: OLEIC ACID / オレイン酸 ChemComp-OLC: (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate / L-グリセロ-ル3-オレア-ト ChemComp-OLB: (2S)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate / 1-O-オレオイル-L-グリセロ-ル ChemComp-NA: Unknown entry / ナトリウムカチオン ChemComp-HOH: WATER ChemComp-NO3: NITRATE ION ChemComp-CA*: Unknown entry / カルシウムジカチオン
由来	homo sapiens (ヒト) parengyodontium album (菌類)
キーワード	MEMBRANE PROTEIN / A2A Adenosine Receptor / HYDROLASE