EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Conformational ensemble of yeast ATP synthase at low pH reveals unique intermediates and plasticity in F-F coupling.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 31, Issue 4, Page 657-666, Year 2024
掲載日	2024年2月5日
著者	Stuti Sharma / Min Luo / Hiral Patel / David M Mueller / Maofu Liao /
PubMed 要旨	Mitochondrial adenosine triphosphate (ATP) synthase uses the proton gradient across the inner mitochondrial membrane to synthesize ATP. Structural and single molecule studies conducted mostly at ...Mitochondrial adenosine triphosphate (ATP) synthase uses the proton gradient across the inner mitochondrial membrane to synthesize ATP. Structural and single molecule studies conducted mostly at neutral or basic pH have provided details of the reaction mechanism of ATP synthesis. However, pH of the mitochondrial matrix is slightly acidic during hypoxia and pH-dependent conformational changes in the ATP synthase have been reported. Here we use single-particle cryo-EM to analyze the conformational ensemble of the yeast (Saccharomyces cerevisiae) ATP synthase at pH 6. Of the four conformations resolved in this study, three are reaction intermediates. In addition to canonical catalytic dwell and binding dwell structures, we identify two unique conformations with nearly identical positions of the central rotor but different catalytic site conformations. These structures provide new insights into the catalytic mechanism of the ATP synthase and highlight elastic coupling between the catalytic and proton translocating domains.
リンク	Nat Struct Mol Biol / PubMed:38316880
手法	EM (単粒子)
解像度	2.7 - 4.5 Å
構造データ	EMDB-28685: Yeast ATP synthase consensus map in conformation-0 at pH 6 手法: EM (単粒子) / 解像度: 4.5 Å EMDB-28687: Yeast ATP synthase F1-focused map in conformation-0 at pH 6 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-28689: Yeast ATP synthase Fo-focused map in conformation-0 at pH 6 手法: EM (単粒子) / 解像度: 3.5 Å 28809 8f29 EMDB-28809: Yeast ATP synthase map in conformation-1 at pH 6 PDB-8f29: Yeast ATP synthase in conformation-1 at pH 6 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-28811: F1-focused map of yeast ATP synthase in conformation-1, at pH 6 手法: EM (単粒子) / 解像度: 2.7 Å EMDB-28814: Fo and central rotor focused map of yeast ATP synthase in conformation-1, at pH 6 手法: EM (単粒子) / 解像度: 3.7 Å 28835 8f39 EMDB-28835: Yeast ATP synthase map in conformation-2, at pH 6 PDB-8f39: Yeast ATP synthase in conformation-2, at pH 6 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-28836: F1-focused map of yeast ATP synthase in conformation-2, at pH 6 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-28837: Fo and central rotor focused map of yeast ATP synthase in conformation-2, at pH 6 手法: EM (単粒子) / 解像度: 3.7 Å 29250 8fkj EMDB-29250, PDB-8fkj: Yeast ATP Synthase in conformation-3, at pH 6 手法: EM (単粒子) / 解像度: 4.2 Å EMDB-29251: Fo and central rotor focused map of yeast ATP Synthase in conformation-3, at pH 6 手法: EM (単粒子) / 解像度: 4.1 Å 29270 8fl8 EMDB-29270: Yeast ATP Synthase map in presence of MgATP PDB-8fl8: Yeast ATP Synthase structure in presence of MgATP 手法: EM (単粒子) / 解像度: 4.2 Å EMDB-29278: F1-focused map of yeast ATP Synthase in presence of MgATP 手法: EM (単粒子) / 解像度: 3.2 Å
化合物	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-PO4: PHOSPHATE ION / ホスファ-ト ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM
由来	saccharomyces cerevisiae (パン酵母)
キーワード	MEMBRANE PROTEIN / F-type ATP synthase / yeast / mitochondrial / F-type / ATP Synthase