EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural insight into an Arl1-ArfGEF complex involved in Golgi recruitment of a GRIP-domain golgin.
ジャーナル・号・ページ	Nat Commun, Vol. 15, Issue 1, Page 1942, Year 2024
掲載日	2024年3月2日
著者	H Diessel Duan / Bhawik K Jain / Hua Li / Todd R Graham / Huilin Li /
PubMed 要旨	Arl1 is an Arf-like (Arl) GTP-binding protein that interacts with the guanine nucleotide exchange factor Gea2 to recruit the golgin Imh1 to the Golgi. The Arl1-Gea2 complex also binds and activates ...Arl1 is an Arf-like (Arl) GTP-binding protein that interacts with the guanine nucleotide exchange factor Gea2 to recruit the golgin Imh1 to the Golgi. The Arl1-Gea2 complex also binds and activates the phosphatidylserine flippase Drs2 and these functions may be related, although the underlying molecular mechanism is unclear. Here we report high-resolution cryo-EM structures of the full-length Gea2 and the Arl1-Gea2 complex. Gea2 is a large protein with 1459 residues and is composed of six domains (DCB, HUS, SEC7, HDS1-3). We show that Gea2 assembles a stable dimer via an extensive interface involving hydrophobic and electrostatic interactions in the DCB and HUS region. Contrary to the previous report on a Gea2 homolog in which Arl1 binds to the dimerization surface of the DCB domain, implying a disrupted dimer upon Arl1 binding, we find that Arl1 binds to the outside surface of the Gea2 DCB domain, leaving the Gea2 dimer intact. The interaction between Arl1 and Gea2 involves the classic FWY aromatic residue triad as well as two Arl1-specific residues. We show that key mutations that disrupt the Arl1-Gea2 interaction abrogate Imh1 Golgi association. This work clarifies the Arl1-Gea2 interaction and improves our understanding of molecular events in the membrane trafficking.
リンク	Nat Commun / PubMed:38431634 / PubMed Central
手法	EM (単粒子)
解像度	3.3 - 4.2 Å
構造データ	28743 8ezj EMDB-28743, PDB-8ezj: Cryo-EM structure of the S. cerevisiae Arf-like protein Arl1 bound to the Arf guanine nucleotide exchange factor Gea2 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-28744: Cryo-EM consensus map of the S. cerevisiae Arf-like protein Arl1 bound to its effector guanine nucleotide exchange factor Gea2 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-28746: Cryo-EM local map covering Gea2 protomer B HDS domains of the S. cerevisiae Arf-like protein Arl1 bound to the Arf guanine nucleotide exchange factor Gea2 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-28747: Cryo-EM local map covering Gea2 protomer A HDS domains of the S. cerevisiae Arf-like protein Arl1 bound to the Arf guanine nucleotide exchange factor Gea2 手法: EM (単粒子) / 解像度: 3.8 Å 28748 8ezq EMDB-28748, PDB-8ezq: Cryo-EM structure of the S. cerevisiae guanine nucleotide exchange factor Gea2 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-28749: Cryo-EM consensus map of the S. cerevisiae guanine nucleotide exchange factor Gea2 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-28750: Cryo-EM local map covering Gea2 protomer B HDS domains of the S. cerevisiae guanine nucleotide exchange factor Gea2 手法: EM (単粒子) / 解像度: 4.2 Å EMDB-28751: Cryo-EM local map covering Gea2 protomer A HDS domains of the S. cerevisiae guanine nucleotide exchange factor Gea2 手法: EM (単粒子) / 解像度: 4.1 Å
化合物	ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子*YM
由来	saccharomyces cerevisiae (パン酵母)
キーワード	LIPID TRANSPORT / small GTPase / guanine nucleotide exchange factor / membrane trafficking / lipid flippase / trans-Golgi network / protein transport