EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Activation mechanism of the mouse cold-sensing TRPM8 channel by cooling agonist and PIP.
ジャーナル・号・ページ	Science, Vol. 378, Issue 6616, Page eadd1268, Year 2022
掲載日	2022年10月14日
著者	Ying Yin / Feng Zhang / Shasha Feng / Kevin John Butay / Mario J Borgnia / Wonpil Im / Seok-Yong Lee /
PubMed 要旨	The transient receptor potential melastatin 8 (TRPM8) channel is the primary molecular transducer responsible for the cool sensation elicited by menthol and cold in mammals. TRPM8 activation is ...The transient receptor potential melastatin 8 (TRPM8) channel is the primary molecular transducer responsible for the cool sensation elicited by menthol and cold in mammals. TRPM8 activation is controlled by cooling compounds together with the membrane lipid phosphatidylinositol 4,5-bisphosphate (PIP). Our knowledge of cold sensation and the therapeutic potential of TRPM8 for neuroinflammatory diseases and pain will be enhanced by understanding the structural basis of cooling agonist- and PIP-dependent TRPM8 activation. We present cryo-electron microscopy structures of mouse TRPM8 in closed, intermediate, and open states along the ligand- and PIP-dependent gating pathway. Our results uncover two discrete agonist sites, state-dependent rearrangements in the gate positions, and a disordered-to-ordered transition of the gate-forming S6-elucidating the molecular basis of chemically induced cool sensation in mammals.
リンク	Science / PubMed:36227998 / PubMed Central
手法	EM (単粒子)
解像度	3.07 - 3.59 Å
構造データ	EMDB-27889: Mouse TRPM8 structure determined in the ligand- and PI(4,5)P2-free condition, Class II, C1 state with endogenous PI(4,5)P2 bound 手法: EM (単粒子) / 解像度: 3.13 Å EMDB-27890: Mouse TRPM8 structure determined in the ligand- and PI(4,5)P2-free condition, Class III, putative desensitized state 手法: EM (単粒子) / 解像度: 3.43 Å 27891 8e4l EMDB-27891, PDB-8e4l: The open state mouse TRPM8 structure in complex with the cooling agonist C3, AITC, and PI(4,5)P2 手法: EM (単粒子) / 解像度: 3.32 Å 27892 8e4m EMDB-27892, PDB-8e4m: The intermediate C2-state mouse TRPM8 structure in complex with the cooling agonist C3 and PI(4,5)P2 手法: EM (単粒子) / 解像度: 3.44 Å 27893 8e4n EMDB-27893, PDB-8e4n: The closed C1-state mouse TRPM8 structure in complex with PI(4,5)P2 手法: EM (単粒子) / 解像度: 3.07 Å 27894 8e4o EMDB-27894, PDB-8e4o: The closed C1-state mouse TRPM8 structure in complex with putative PI(4,5)P2 手法: EM (単粒子) / 解像度: 3.43 Å 27895 8e4p EMDB-27895, PDB-8e4p: Mouse TRPM8 structure determined in the ligand- and PI(4,5)P2-free condition, Class I , C0 state 手法: EM (単粒子) / 解像度: 3.59 Å 27896 8e4q EMDB-27896, PDB-8e4q: The closed C0-state flycatcher TRPM8 structure in complex with PI(4,5)P2 手法: EM (単粒子) / 解像度: 3.51 Å
化合物	ChemComp-CA: Unknown entry / カルシウムジカチオン ChemComp-UL9: 3-isothiocyanatoprop-1-ene / アリルイソチオシアナ-ト ChemComp-ULO: nonyl(oxo)di(propan-2-yl)-lambda~5~-phosphane ChemComp-PIO: [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate / 1-O-(1-O,2-O-ジオクタノイル-L-グリセロ-3-ホスホ)-D-myo-イノシト-ル4,5-ビスりん酸 ChemComp-Y01: CHOLESTEROL HEMISUCCINATE / コレステリルヘミスクシナ-ト
由来	mus musculus (ハツカネズミ) Mus musculus ficedula albicollis (シロエリヒタキ)
キーワード	MEMBRANE PROTEIN / TRPM8 / menthol receptor / cold receptor / PI(4 / 5)P2 / cooling agonists / temperature sensing / ion channel / sensory transduction / transient receptor potential ion channel / cold sensor