Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Activation mechanism of the mouse cold-sensing TRPM8 channel by cooling agonist and PIP.
Journal, issue, pages	Science, Vol. 378, Issue 6616, Page eadd1268, Year 2022
Publish date	Oct 14, 2022
Authors	Ying Yin / Feng Zhang / Shasha Feng / Kevin John Butay / Mario J Borgnia / Wonpil Im / Seok-Yong Lee /
PubMed Abstract	The transient receptor potential melastatin 8 (TRPM8) channel is the primary molecular transducer responsible for the cool sensation elicited by menthol and cold in mammals. TRPM8 activation is ...The transient receptor potential melastatin 8 (TRPM8) channel is the primary molecular transducer responsible for the cool sensation elicited by menthol and cold in mammals. TRPM8 activation is controlled by cooling compounds together with the membrane lipid phosphatidylinositol 4,5-bisphosphate (PIP). Our knowledge of cold sensation and the therapeutic potential of TRPM8 for neuroinflammatory diseases and pain will be enhanced by understanding the structural basis of cooling agonist- and PIP-dependent TRPM8 activation. We present cryo-electron microscopy structures of mouse TRPM8 in closed, intermediate, and open states along the ligand- and PIP-dependent gating pathway. Our results uncover two discrete agonist sites, state-dependent rearrangements in the gate positions, and a disordered-to-ordered transition of the gate-forming S6-elucidating the molecular basis of chemically induced cool sensation in mammals.
External links	Science / PubMed:36227998 / PubMed Central
Methods	EM (single particle)
Resolution	3.07 - 3.59 Å
Structure data	EMDB-27889: Mouse TRPM8 structure determined in the ligand- and PI(4,5)P2-free condition, Class II, C1 state with endogenous PI(4,5)P2 bound Method: EM (single particle) / Resolution: 3.13 Å EMDB-27890: Mouse TRPM8 structure determined in the ligand- and PI(4,5)P2-free condition, Class III, putative desensitized state Method: EM (single particle) / Resolution: 3.43 Å 27891 8e4l EMDB-27891, PDB-8e4l: The open state mouse TRPM8 structure in complex with the cooling agonist C3, AITC, and PI(4,5)P2 Method: EM (single particle) / Resolution: 3.32 Å 27892 8e4m EMDB-27892, PDB-8e4m: The intermediate C2-state mouse TRPM8 structure in complex with the cooling agonist C3 and PI(4,5)P2 Method: EM (single particle) / Resolution: 3.44 Å 27893 8e4n EMDB-27893, PDB-8e4n: The closed C1-state mouse TRPM8 structure in complex with PI(4,5)P2 Method: EM (single particle) / Resolution: 3.07 Å 27894 8e4o EMDB-27894, PDB-8e4o: The closed C1-state mouse TRPM8 structure in complex with putative PI(4,5)P2 Method: EM (single particle) / Resolution: 3.43 Å 27895 8e4p EMDB-27895, PDB-8e4p: Mouse TRPM8 structure determined in the ligand- and PI(4,5)P2-free condition, Class I , C0 state Method: EM (single particle) / Resolution: 3.59 Å 27896 8e4q EMDB-27896, PDB-8e4q: The closed C0-state flycatcher TRPM8 structure in complex with PI(4,5)P2 Method: EM (single particle) / Resolution: 3.51 Å
Chemicals	ChemComp-CA: Unknown entry ChemComp-UL9: 3-isothiocyanatoprop-1-ene ChemComp-ULO: nonyl(oxo)di(propan-2-yl)-lambda~5~-phosphane ChemComp-PIO: [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate ChemComp-Y01: CHOLESTEROL HEMISUCCINATE
Source	mus musculus (house mouse) Mus musculus ficedula albicollis (Collared flycatcher)
Keywords	MEMBRANE PROTEIN / TRPM8 / menthol receptor / cold receptor / PI(4 / 5)P2 / cooling agonists / temperature sensing / ion channel / sensory transduction / transient receptor potential ion channel / cold sensor