EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Human PRPS1 filaments stabilize allosteric sites to regulate activity.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 30, Issue 3, Page 391-402, Year 2023
掲載日	2023年2月6日
著者	Kelli L Hvorecny / Kenzee Hargett / Joel D Quispe / Justin M Kollman /
PubMed 要旨	The universally conserved enzyme phosphoribosyl pyrophosphate synthetase (PRPS) assembles filaments in evolutionarily diverse organisms. PRPS is a key regulator of nucleotide metabolism, and ...The universally conserved enzyme phosphoribosyl pyrophosphate synthetase (PRPS) assembles filaments in evolutionarily diverse organisms. PRPS is a key regulator of nucleotide metabolism, and mutations in the human enzyme PRPS1 lead to a spectrum of diseases. Here we determine structures of human PRPS1 filaments in active and inhibited states, with fixed assembly contacts accommodating both conformations. The conserved assembly interface stabilizes the binding site for the essential activator phosphate, increasing activity in the filament. Some disease mutations alter assembly, supporting the link between filament stability and activity. Structures of active PRPS1 filaments turning over substrate also reveal coupling of catalysis in one active site with product release in an adjacent site. PRPS1 filaments therefore provide an additional layer of allosteric control, conserved throughout evolution, with likely impact on metabolic homeostasis. Stabilization of allosteric binding sites by polymerization adds to the growing diversity of assembly-based enzyme regulatory mechanisms.
リンク	Nat Struct Mol Biol / PubMed:36747094 / PubMed Central
手法	EM (単粒子)
解像度	2.0 - 3.2 Å
構造データ	27279 8dbc EMDB-27279, PDB-8dbc: Human PRPS1 with Phosphate; Hexamer 手法: EM (単粒子) / 解像度: 3.2 Å 27280 8dbd EMDB-27280, PDB-8dbd: Human PRPS1 with Phosphate; Filament Interface 手法: EM (単粒子) / 解像度: 3.2 Å 27281 8dbe EMDB-27281, PDB-8dbe: Human PRPS1 with ADP; Hexamer 手法: EM (単粒子) / 解像度: 2.1 Å 27282 8dbf EMDB-27282, PDB-8dbf: Human PRPS1 with ADP; Filament Interface 手法: EM (単粒子) / 解像度: 2.2 Å 27283 8dbg EMDB-27283, PDB-8dbg: Human PRPS1 with Phosphate and ATP; Hexamer 手法: EM (単粒子) / 解像度: 2.2 Å 27284 8dbh EMDB-27284, PDB-8dbh: Human PRPS1 with Phosphate and ATP; Filament Interface 手法: EM (単粒子) / 解像度: 2.2 Å 27285 8dbi EMDB-27285, PDB-8dbi: Human PRPS1 with Phosphate, ATP, and R5P; Hexamer 手法: EM (単粒子) / 解像度: 2.0 Å 27286 8dbj EMDB-27286, PDB-8dbj: Human PRPS1 with Phosphate, ATP, and R5P; Filament Interface 手法: EM (単粒子) / 解像度: 2.0 Å 27287 8dbk EMDB-27287, PDB-8dbk: Human PRPS1 with Phosphate, ATP, and R5P; Hexamer with resolved catalytic loops 手法: EM (単粒子) / 解像度: 2.1 Å EMDB-27288: Human PRPS1 with Phosphate, ATP, and R5P; Hexamer with resolved catalytic loops 手法: EM (単粒子) / 解像度: 2.1 Å 27289 8dbl EMDB-27289, PDB-8dbl: Human PRPS1 with Phosphate and PRPP; Hexamer 手法: EM (単粒子) / 解像度: 2.4 Å 27290 8dbm EMDB-27290, PDB-8dbm: Human PRPS1 with Phosphate and PRPP; Filament Interface 手法: EM (単粒子) / 解像度: 2.4 Å 27291 8dbn EMDB-27291, PDB-8dbn: Human PRPS1-E307A engineered mutation with Phosphate, ATP, and R5P; Hexamer 手法: EM (単粒子) / 解像度: 2.4 Å EMDB-27292: Human PRPS1-E307A engineered mutation with Phosphate, ATP, and R5P; Symmetry-expanded Hexamer 手法: EM (単粒子) / 解像度: 2.6 Å 27293 8dbo EMDB-27293, PDB-8dbo: Human PRPS1-E307A engineered mutation with ADP; Hexamer 手法: EM (単粒子) / 解像度: 2.5 Å EMDB-27294: Human PRPS1-E307A engineered mutation with ADP; Symmetry-expanded Hexamer 1 手法: EM (単粒子) / 解像度: 2.5 Å EMDB-27295: Human PRPS1-E307A engineered mutation with ADP; Symmetry-expanded Hexamer 2 手法: EM (単粒子) / 解像度: 2.6 Å
化合物	ChemComp-PO4: PHOSPHATE ION / ホスファ-ト ChemComp-HSX: 5-O-phosphono-alpha-D-ribofuranose / α-D-リボフラノ-ス5-りん酸 ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-HOH: WATER ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-PRP: 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose / PRPP ChemComp-AMP: ADENOSINE MONOPHOSPHATE / AMP / AMP*YM
由来	homo sapiens (ヒト)
キーワード	TRANSFERASE / phosphoribosyl pyrophosphate synthetase / phosphate / phosphate activator / ADP inhibitor / ATP substrate / ATP and R5P substrates / catalytic loop / PRPP product / ATP/R5P substrate / Filament-breaking mutation