EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Lipid flipping in the omega-3 fatty-acid transporter.
ジャーナル・号・ページ	Nat Commun, Vol. 14, Issue 1, Page 2571, Year 2023
掲載日	2023年5月8日
著者	Chi Nguyen / Hsiang-Ting Lei / Louis Tung Faat Lai / Marc J Gallenito / Xuelang Mu / Doreen Matthies / Tamir Gonen /
PubMed 要旨	Mfsd2a is the transporter for docosahexaenoic acid (DHA), an omega-3 fatty acid, across the blood brain barrier (BBB). Defects in Mfsd2a are linked to ailments from behavioral and motor dysfunctions ...Mfsd2a is the transporter for docosahexaenoic acid (DHA), an omega-3 fatty acid, across the blood brain barrier (BBB). Defects in Mfsd2a are linked to ailments from behavioral and motor dysfunctions to microcephaly. Mfsd2a transports long-chain unsaturated fatty-acids, including DHA and α-linolenic acid (ALA), that are attached to the zwitterionic lysophosphatidylcholine (LPC) headgroup. Even with the recently determined structures of Mfsd2a, the molecular details of how this transporter performs the energetically unfavorable task of translocating and flipping lysolipids across the lipid bilayer remains unclear. Here, we report five single-particle cryo-EM structures of Danio rerio Mfsd2a (drMfsd2a): in the inward-open conformation in the ligand-free state and displaying lipid-like densities modeled as ALA-LPC at four distinct positions. These Mfsd2a snapshots detail the flipping mechanism for lipid-LPC from outer to inner membrane leaflet and release for membrane integration on the cytoplasmic side. These results also map Mfsd2a mutants that disrupt lipid-LPC transport and are associated with disease.
リンク	Nat Commun / PubMed:37156797 / PubMed Central
手法	EM (単粒子)
解像度	2.9 - 4.1 Å
構造データ	27148 8d2s EMDB-27148, PDB-8d2s: Zebrafish MFSD2A isoform B in inward open ligand bound conformation 手法: EM (単粒子) / 解像度: 2.9 Å 27149 8d2t EMDB-27149, PDB-8d2t: Zebrafish MFSD2A isoform B in inward open ligand-free conformation 手法: EM (単粒子) / 解像度: 3.4 Å 27150 8d2u EMDB-27150, PDB-8d2u: Zebrafish MFSD2A isoform B in inward open ligand 1A conformation 手法: EM (単粒子) / 解像度: 3.3 Å 27151 8d2v EMDB-27151, PDB-8d2v: Zebrafish MFSD2A isoform B in inward open ligand 1B conformation 手法: EM (単粒子) / 解像度: 4.1 Å 27152 8d2w EMDB-27152, PDB-8d2w: Zebrafish MFSD2A isoform B in inward open ligand 2B conformation 手法: EM (単粒子) / 解像度: 3.4 Å 27153 8d2x EMDB-27153, PDB-8d2x: Zebrafish MFSD2A isoform B in inward open ligand 3C conformation 手法: EM (単粒子) / 解像度: 3.4 Å
化合物	ChemComp-ZGS: [(2~{R})-2-oxidanyl-3-[oxidanyl-[2-(trimethyl-$l^{4}-azanyl)ethoxy]phosphoryl]oxy-propyl] (9~{Z},12~{Z},15~{Z})-octadeca-9,12,15-trienoate / 1-α-リノレノイルリソホスファチジルコリン ChemComp-LMT: DODECYL-BETA-D-MALTOSIDE / ドデシルβ-D-マルトシド / 可溶化剤YM ChemComp-NA: Unknown entry / ナトリウムカチオン ChemComp-HOH*: WATER
由来	danio rerio (ゼブラフィッシュ) mus musculus (ハツカネズミ)
キーワード	LIPID TRANSPORT / omega-3 fatty acid / membrane protein / Fab