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-Structure paper
タイトル | Cryo-EM structure of a DNA-PK trimer: higher order oligomerisation in NHEJ. |
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ジャーナル・号・ページ | Structure, Vol. 31, Issue 8, Page 895-902.e3, Year 2023 |
掲載日 | 2023年8月3日 |
著者 | Steven W Hardwick / Antonia Kefala Stavridi / Dimitri Y Chirgadze / Taiana Maia De Oliveira / Jean-Baptiste Charbonnier / Virginie Ropars / Katheryn Meek / Tom L Blundell / Amanda K Chaplin / |
PubMed 要旨 | The ability of humans to maintain the integrity of the genome is imperative for cellular survival. DNA double-strand breaks (DSBs) are considered the most critical type of DNA lesion, which can ...The ability of humans to maintain the integrity of the genome is imperative for cellular survival. DNA double-strand breaks (DSBs) are considered the most critical type of DNA lesion, which can ultimately lead to diseases including cancer. Non-homologous end joining (NHEJ) is one of two core mechanisms utilized to repair DSBs. DNA-PK is a key component in this process and has recently been shown to form alternate long-range synaptic dimers. This has led to the proposal that these complexes can be formed before transitioning to a short-range synaptic complex. Here we present cryo-EM data representing an NHEJ supercomplex consisting of a trimer of DNA-PK in complex with XLF, XRCC4, and DNA Ligase IV. This trimer represents a complex of both long-range synaptic dimers. We discuss the potential role of the trimeric structure, and possible higher order oligomers, as structural intermediates in the NHEJ mechanism, or as functional DNA repair centers. |
リンク | Structure / PubMed:37311458 |
手法 | EM (単粒子) |
解像度 | 7.76 Å |
構造データ | EMDB-16145, PDB-8bot: |
由来 |
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キーワード | DNA BINDING PROTEIN / NHEJ / DNA-PK / DNA-PKcs / Ku70 / Ku80 / XLF / DNA repair |