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-Structure paper
タイトル | Structural basis for TBP displacement from TATA box DNA by the Swi2/Snf2 ATPase Mot1. |
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ジャーナル・号・ページ | Nat Struct Mol Biol, Vol. 30, Issue 5, Page 640-649, Year 2023 |
掲載日 | 2023年4月27日 |
著者 | Stephan Woike / Sebastian Eustermann / James Jung / Simon Josef Wenzl / Götz Hagemann / Joseph Bartho / Katja Lammens / Agata Butryn / Franz Herzog / Karl-Peter Hopfner / |
PubMed 要旨 | The Swi2/Snf2 family transcription regulator Modifier of Transcription 1 (Mot1) uses adenosine triphosphate (ATP) to dissociate and reallocate the TATA box-binding protein (TBP) from and between ...The Swi2/Snf2 family transcription regulator Modifier of Transcription 1 (Mot1) uses adenosine triphosphate (ATP) to dissociate and reallocate the TATA box-binding protein (TBP) from and between promoters. To reveal how Mot1 removes TBP from TATA box DNA, we determined cryogenic electron microscopy structures that capture different states of the remodeling reaction. The resulting molecular video reveals how Mot1 dissociates TBP in a process that, intriguingly, does not require DNA groove tracking. Instead, the motor grips DNA in the presence of ATP and swings back after ATP hydrolysis, moving TBP to a thermodynamically less stable position on DNA. Dislodged TBP is trapped by a chaperone element that blocks TBP's DNA binding site. Our results show how Swi2/Snf2 proteins can remodel protein-DNA complexes through DNA bending without processive DNA tracking and reveal mechanistic similarities to RNA gripping DEAD box helicases and RIG-I-like immune sensors. |
リンク | Nat Struct Mol Biol / PubMed:37106137 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.8 - 5.1 Å |
構造データ | EMDB-14534, PDB-7z7n: EMDB-14554: Mot1:TBP - product state EMDB-14562, PDB-7z8s: EMDB-14584, PDB-7zb5: EMDB-14762, PDB-7zke: |
化合物 | ChemComp-MG: ChemComp-ADP: ChemComp-BEF: |
由来 |
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キーワード | TRANSCRIPTION / Swi2/Snf2 protein / remodeler / transcription initiation |