EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM structures of light-harvesting 2 complexes from reveal the molecular origin of absorption tuning.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 119, Issue 43, Page e2210109119, Year 2022
掲載日	2022年10月25日
著者	Pu Qian / Cam T Nguyen-Phan / Alastair T Gardiner / Tristan I Croll / Aleksander W Roszak / June Southall / Philip J Jackson / Cvetelin Vasilev / Pablo Castro-Hartmann / Kasim Sader / C Neil Hunter / Richard J Cogdell /
PubMed 要旨	The genomes of some purple photosynthetic bacteria contain a multigene family encoding a series of α- and β-polypeptides that together form a heterogeneous antenna of light-harvesting 2 (LH2) ...The genomes of some purple photosynthetic bacteria contain a multigene family encoding a series of α- and β-polypeptides that together form a heterogeneous antenna of light-harvesting 2 (LH2) complexes. To unravel this complexity, we generated four sets of deletion mutants in , each encoding a single type of gene pair and enabling the purification of complexes designated as PucA-LH2, PucB-LH2, PucD-LH2, and PucE-LH2. The structures of all four purified LH2 complexes were determined by cryogenic electron microscopy (cryo-EM) at resolutions ranging from 2.7 to 3.6 Å. Uniquely, each of these complexes contains a hitherto unknown polypeptide, γ, that forms an extended undulating ribbon that lies in the plane of the membrane and that encloses six of the nine LH2 αβ-subunits. The γ-subunit, which is located near to the cytoplasmic side of the complex, breaks the C9 symmetry of the LH2 complex and binds six extra bacteriochlorophylls (BChls) that enhance the 800-nm absorption of each complex. The structures show that all four complexes have two complete rings of BChls, conferring absorption bands centered at 800 and 850 nm on the PucA-LH2, PucB-LH2, and PucE-LH2 complexes, but, unusually, the PucD-LH2 antenna has only a single strong near-infared (NIR) absorption peak at 803 nm. Comparison of the cryo-EM structures of these LH2 complexes reveals altered patterns of hydrogen bonds between LH2 αβ-side chains and the bacteriochlorin rings, further emphasizing the major role that H bonds play in spectral tuning of bacterial antenna complexes.
リンク	Proc Natl Acad Sci U S A / PubMed:36251992 / PubMed Central
手法	EM (単粒子)
解像度	2.7 - 3.6 Å
構造データ	14633 7zcu EMDB-14633, PDB-7zcu: PucA-LH2 complex from Rps. palustris 手法: EM (単粒子) / 解像度: 2.7 Å 14650 7zdi EMDB-14650, PDB-7zdi: PucB-LH2 complex from Rps. palustris 手法: EM (単粒子) / 解像度: 2.9 Å 14682 7ze3 EMDB-14682, PDB-7ze3: PucD-LH2 complex from Rps. palustris 手法: EM (単粒子) / 解像度: 2.7 Å 14685 7ze8 EMDB-14685, PDB-7ze8: PucE-LH2 complex from Rps. palustris 手法: EM (単粒子) / 解像度: 3.6 Å
化合物	ChemComp-BCL: BACTERIOCHLOROPHYLL A / バクテリオクロロフィル ChemComp-IRM: 1,2-Dihydro-psi,psi-caroten-1-ol / ロドピン / Rhodopin ChemComp-HOH: WATER / 水 ChemComp-ZE0: (3'E)-3',4'-didehydro-1,2-dihydro-psi,psi-caroten-1-ol / 1,2-ジヒドロ-3′,4′-ジデヒドロ-ψ,ψ-カロテン-1-オ-ル
由来	rhodopseudomonas palustris atcc 17001 (光合成細菌) rhodopseudomonas palustris (光合成細菌)
キーワード	PHOTOSYNTHESIS (光合成) / light harvesting complex 2 / LH2 / Rps. palustris / purple bacteria (紅色細菌) / cryo-EM (低温電子顕微鏡法) / single particle analysis (単粒子解析法)