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- PDB-7zcu: PucA-LH2 complex from Rps. palustris -

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Basic information

Entry
Database: PDB / ID: 7zcu
TitlePucA-LH2 complex from Rps. palustris
Components
  • (Light-harvesting protein B-800-850 ...) x 2
  • PucA-LH2-gamma
KeywordsPHOTOSYNTHESIS / light harvesting complex 2 / LH2 / Rps. palustris / purple bacteria / cryo-EM / single particle analysis
Function / homology
Function and homology information


organelle inner membrane / plasma membrane light-harvesting complex / bacteriochlorophyll binding / photosynthesis, light reaction / electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity / membrane => GO:0016020 / metal ion binding / plasma membrane
Similarity search - Function
Antenna complex, beta subunit, conserved site / Antenna complexes beta subunits signature. / Antenna complex, alpha subunit / Antenna complex, beta domain superfamily / Antenna complex, alpha subunit conserved site / Antenna complexes alpha subunits signature. / Light-harvesting protein B beta chain / Antenna complex, alpha/beta subunit / Light-harvesting complex / Antenna complex alpha/beta subunit
Similarity search - Domain/homology
BACTERIOCHLOROPHYLL A / 1,2-Dihydro-psi,psi-caroten-1-ol / Light-harvesting protein B-800-850 alpha chain / Light-harvesting protein B-800-850 beta chain / Uncharacterized protein
Similarity search - Component
Biological speciesRhodopseudomonas palustris ATCC 17001 (phototrophic)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.7 Å
AuthorsQian, P. / Cogdell, R.J.
Funding support United Kingdom, 1items
OrganizationGrant numberCountry
Biotechnology and Biological Sciences Research Council (BBSRC)BB/N016734/1 United Kingdom
Citation
Journal: Proc Natl Acad Sci U S A / Year: 2022
Title: Cryo-EM structures of light-harvesting 2 complexes from reveal the molecular origin of absorption tuning.
Authors: Pu Qian / Cam T Nguyen-Phan / Alastair T Gardiner / Tristan I Croll / Aleksander W Roszak / June Southall / Philip J Jackson / Cvetelin Vasilev / Pablo Castro-Hartmann / Kasim Sader / C Neil ...Authors: Pu Qian / Cam T Nguyen-Phan / Alastair T Gardiner / Tristan I Croll / Aleksander W Roszak / June Southall / Philip J Jackson / Cvetelin Vasilev / Pablo Castro-Hartmann / Kasim Sader / C Neil Hunter / Richard J Cogdell /
Abstract: The genomes of some purple photosynthetic bacteria contain a multigene family encoding a series of α- and β-polypeptides that together form a heterogeneous antenna of light-harvesting 2 (LH2) ...The genomes of some purple photosynthetic bacteria contain a multigene family encoding a series of α- and β-polypeptides that together form a heterogeneous antenna of light-harvesting 2 (LH2) complexes. To unravel this complexity, we generated four sets of deletion mutants in , each encoding a single type of gene pair and enabling the purification of complexes designated as PucA-LH2, PucB-LH2, PucD-LH2, and PucE-LH2. The structures of all four purified LH2 complexes were determined by cryogenic electron microscopy (cryo-EM) at resolutions ranging from 2.7 to 3.6 Å. Uniquely, each of these complexes contains a hitherto unknown polypeptide, γ, that forms an extended undulating ribbon that lies in the plane of the membrane and that encloses six of the nine LH2 αβ-subunits. The γ-subunit, which is located near to the cytoplasmic side of the complex, breaks the C9 symmetry of the LH2 complex and binds six extra bacteriochlorophylls (BChls) that enhance the 800-nm absorption of each complex. The structures show that all four complexes have two complete rings of BChls, conferring absorption bands centered at 800 and 850 nm on the PucA-LH2, PucB-LH2, and PucE-LH2 complexes, but, unusually, the PucD-LH2 antenna has only a single strong near-infared (NIR) absorption peak at 803 nm. Comparison of the cryo-EM structures of these LH2 complexes reveals altered patterns of hydrogen bonds between LH2 αβ-side chains and the bacteriochlorin rings, further emphasizing the major role that H bonds play in spectral tuning of bacterial antenna complexes.
#1: Journal: Acta Crystallogr., Sect. D: Biol. Crystallogr. / Year: 2018
Title: Real-space refinement in PHENIX for cryo-EM and crystallography
Authors: Qian, P. / Cogdell, R.J.
#2: Journal: To Be Published
Title: Cryo-EM structures of light harvesting complex 2 from Rhodopseudomonas palustris: a molecular origin of spectroscopic variation
Authors: Qian, P. / Cogdell, R.j.
History
DepositionMar 28, 2022Deposition site: PDBE / Processing site: PDBE
Revision 1.0Oct 12, 2022Provider: repository / Type: Initial release
Revision 1.1Nov 23, 2022Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Light-harvesting protein B-800-850 alpha chain
B: Light-harvesting protein B-800-850 beta chain
S: PucA-LH2-gamma
C: Light-harvesting protein B-800-850 alpha chain
D: Light-harvesting protein B-800-850 beta chain
E: Light-harvesting protein B-800-850 alpha chain
F: Light-harvesting protein B-800-850 beta chain
G: Light-harvesting protein B-800-850 alpha chain
H: Light-harvesting protein B-800-850 beta chain
I: Light-harvesting protein B-800-850 alpha chain
J: Light-harvesting protein B-800-850 beta chain
K: Light-harvesting protein B-800-850 alpha chain
L: Light-harvesting protein B-800-850 beta chain
M: Light-harvesting protein B-800-850 alpha chain
N: Light-harvesting protein B-800-850 beta chain
O: Light-harvesting protein B-800-850 alpha chain
P: Light-harvesting protein B-800-850 beta chain
Q: Light-harvesting protein B-800-850 alpha chain
R: Light-harvesting protein B-800-850 beta chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)151,50661
Polymers116,43219
Non-polymers35,07442
Water1629
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: electron microscopy, 2d projection and 3D reconstruction.
TypeNameSymmetry operationNumber
identity operation1_5551
Buried area105160 Å2
ΔGint-779 kcal/mol
Surface area38970 Å2
MethodPISA

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Components

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Light-harvesting protein B-800-850 ... , 2 types, 18 molecules ACEGIKMOQBDFHJLNPR

#1: Protein
Light-harvesting protein B-800-850 alpha chain / PucA-LH2-alpha / Light-harvesting protein B-800-850 subunit alpha


Mass: 6443.515 Da / Num. of mol.: 9 / Source method: isolated from a natural source
Source: (natural) Rhodopseudomonas palustris ATCC 17001 (phototrophic)
Strain: ATCC 17001 / References: UniProt: A0A2R4GUT4
#2: Protein/peptide
Light-harvesting protein B-800-850 beta chain / PucA-LH2-beta / Light-harvesting protein B-800-850 subunit beta


Mass: 5288.061 Da / Num. of mol.: 9 / Source method: isolated from a natural source
Source: (natural) Rhodopseudomonas palustris ATCC 17001 (phototrophic)
Strain: ATCC 17001 / References: UniProt: A0A2R4GUZ8

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Protein , 1 types, 1 molecules S

#3: Protein PucA-LH2-gamma / Light-harvesting protein B-800-850 subunit gamma


Mass: 10848.179 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Rhodopseudomonas palustris ATCC 17001 (phototrophic)
Strain: ATCC 17001 / References: UniProt: Q6N9P5

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Non-polymers , 3 types, 51 molecules

#4: Chemical...
ChemComp-BCL / BACTERIOCHLOROPHYLL A / Bacteriochlorophyll


Mass: 911.504 Da / Num. of mol.: 33 / Source method: obtained synthetically / Formula: C55H74MgN4O6 / Feature type: SUBJECT OF INVESTIGATION
#5: Chemical
ChemComp-IRM / 1,2-Dihydro-psi,psi-caroten-1-ol / Rhodopin / (6~{E},8~{E},10~{E},12~{E},14~{E},16~{E},18~{E},20~{E},22~{E},24~{E},26~{E})-2,6,10,14,19,23,27,31-octamethyldotriaconta-6,8,10,12,14,16,18,20,22,24,26,30-dodecaen-2-ol / Rhodopin


Mass: 554.888 Da / Num. of mol.: 9 / Source method: obtained synthetically / Formula: C40H58O
#6: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 9 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: PucA-LH2 from Rps. palustris / Type: COMPLEX / Details: Delt-pucBA(bced) / Entity ID: #1-#3 / Source: NATURAL
Source (natural)Organism: Rhodopseudomonas palustris ATCC 17001 (phototrophic)
Buffer solutionpH: 8 / Details: 0.1% LDAO in 20 mM Tris.Cl pH 8.0
Buffer componentConc.: 20 mM / Name: Tris.HCl / Formula: C4H11NO3
SpecimenConc.: 7 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES / Details: protein was purified using LDAO detergent.
Specimen supportGrid material: COPPER / Grid mesh size: 300 divisions/in. / Grid type: Quantifoil R1.2/1.3
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 277 K
Details: Chamber humidity: 100%; Chamber temperature: 4 oC; Blotting time: 2.5; Blotting force: 3; Wait time: 30 sec

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELDBright-field microscopy / Nominal magnification: 130000 X / Nominal defocus max: 2400 nm / Nominal defocus min: 800 nm / Cs: 2.7 mm / C2 aperture diameter: 50 µm / Alignment procedure: COMA FREE
Specimen holderCryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Temperature (min): 80 K
Image recordingAverage exposure time: 1.4 sec. / Electron dose: 42.03 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Num. of grids imaged: 1 / Num. of real images: 4865 / Details: Images were collected in AFIS mode.
EM imaging opticsEnergyfilter name: GIF Bioquantum / Energyfilter slit width: 20 eV

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Processing

Software
NameVersionClassificationNB
phenix.real_space_refinedev_4386refinement
PHENIXdev_4386refinement
EM software
IDNameVersionCategory
2EPUimage acquisition
4CTFFIND4CTF correction
11RELION3.1final Euler assignment
12RELION3.1classification
13RELION3.13D reconstruction
CTF correctionType: NONE
Particle selectionNum. of particles selected: 2407413
SymmetryPoint symmetry: C1 (asymmetric)
3D reconstructionResolution: 2.7 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 368671 / Algorithm: BACK PROJECTION / Num. of class averages: 1 / Symmetry type: POINT
RefinementCross valid method: NONE
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
Displacement parametersBiso mean: 29.91 Å2
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.003610612
ELECTRON MICROSCOPYf_angle_d0.91714900
ELECTRON MICROSCOPYf_chiral_restr0.04161516
ELECTRON MICROSCOPYf_plane_restr0.00991925
ELECTRON MICROSCOPYf_dihedral_angle_d13.9763626

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