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-Structure paper
タイトル | In situ structure of the red algal phycobilisome-PSII-PSI-LHC megacomplex. |
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ジャーナル・号・ページ | Nature, Vol. 616, Issue 7955, Page 199-206, Year 2023 |
掲載日 | 2023年3月15日 |
![]() | Xin You / Xing Zhang / Jing Cheng / Yanan Xiao / Jianfei Ma / Shan Sun / Xinzheng Zhang / Hong-Wei Wang / Sen-Fang Sui / ![]() |
PubMed 要旨 | In oxygenic photosynthetic organisms, light energy is captured by antenna systems and transferred to photosystem II (PSII) and photosystem I (PSI) to drive photosynthesis. The antenna systems of red ...In oxygenic photosynthetic organisms, light energy is captured by antenna systems and transferred to photosystem II (PSII) and photosystem I (PSI) to drive photosynthesis. The antenna systems of red algae consist of soluble phycobilisomes (PBSs) and transmembrane light-harvesting complexes (LHCs). Excitation energy transfer pathways from PBS to photosystems remain unclear owing to the lack of structural information. Here we present in situ structures of PBS-PSII-PSI-LHC megacomplexes from the red alga Porphyridium purpureum at near-atomic resolution using cryogenic electron tomography and in situ single-particle analysis, providing interaction details between PBS, PSII and PSI. The structures reveal several unidentified and incomplete proteins and their roles in the assembly of the megacomplex, as well as a huge and sophisticated pigment network. This work provides a solid structural basis for unravelling the mechanisms of PBS-PSII-PSI-LHC megacomplex assembly, efficient energy transfer from PBS to the two photosystems, and regulation of energy distribution between PSII and PSI. |
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手法 | EM (単粒子) |
解像度 | 3.3 - 6.3 Å |
構造データ | EMDB-33558, PDB-7y1a: EMDB-33605, PDB-7y4l: EMDB-33618, PDB-7y5e: EMDB-33658, PDB-7y7a: ![]() EMDB-33669: In situ double-PBS-PSII-PSI-LHCs megacomplex from red alga Porphyridium purpureum. |
化合物 | ![]() ChemComp-PEB: ![]() ChemComp-PUB: ![]() ChemComp-CYC: ![]() ChemComp-BCR: ![]() ChemComp-CL: ![]() ChemComp-CLA: ![]() ChemComp-LHG: ![]() ChemComp-OEX: ![]() ChemComp-FE: ![]() ChemComp-PHO: ![]() ChemComp-LMG: ![]() ChemComp-BCT: ![]() ChemComp-PL9: ![]() ChemComp-DGD: ![]() ChemComp-HEM: ![]() ChemComp-GOL: ![]() ChemComp-PQN: ![]() ChemComp-SF4: ![]() ChemComp-3XQ: ![]() ChemComp-FES: ![]() ChemComp-BGC: ![]() ChemComp-ZEX: ![]() ChemComp-HOH: |
由来 |
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![]() | PHOTOSYNTHESIS / Lateral hexamer / PBS / complex / Double-PBS-PSII-PSI-LHCs |