EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Mechanistic insights into DNA binding and cleavage by a compact type I-F CRISPR-Cas system in bacteriophage.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 120, Issue 18, Page e2215098120, Year 2023
掲載日	2023年5月2日
著者	Manling Zhang / Ruchao Peng / Qi Peng / Sheng Liu / Zhiteng Li / Yuqin Zhang / Hao Song / Jia Yang / Xiao Xing / Peiyi Wang / Jianxun Qi / George F Gao /
PubMed 要旨	CRISPR-Cas systems are widespread adaptive antiviral systems used in prokaryotes. Some phages, in turn, although have small genomes can economize the use of genetic space to encode compact or ...CRISPR-Cas systems are widespread adaptive antiviral systems used in prokaryotes. Some phages, in turn, although have small genomes can economize the use of genetic space to encode compact or incomplete CRISPR-Cas systems to inhibit the host and establish infection. Phage ICP1, infecting , encodes a compact type I-F CRISPR-Cas system to suppress the antiphage mobile genetic element in the host genome. However, the mechanism by which this compact system recognizes the target DNA and executes interference remains elusive. Here, we present the electron cryo-microscopy (cryo-EM) structures of both apo- and DNA-bound ICP1 surveillance complexes (Aka Csy complex). Unlike most other type I surveillance complexes, the ICP1 Csy complex lacks the Cas11 subunit or a structurally homologous domain, which is crucial for dsDNA binding and Cas3 activation in other type I CRISPR-Cas systems. Structural and functional analyses revealed that the compact ICP1 Csy complex alone is inefficient in binding to dsDNA targets, presumably stalled at a partial R-loop conformation. The presence of Cas2/3 facilitates dsDNA binding and allows effective dsDNA target cleavage. Additionally, we found that Cas2/3 efficiently cleaved the dsDNA target presented by the ICP1 Csy complex, but not vice versa. These findings suggest a unique mechanism for target dsDNA binding and cleavage by the compact phage-derived CRISPR-Cas system.
リンク	Proc Natl Acad Sci U S A / PubMed:37094126 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2 - 3.5 Å
構造データ	32873 7wwu EMDB-32873, PDB-7wwu: ICP1 Csy complex 手法: EM (単粒子) / 解像度: 3.5 Å 32874 7wwv EMDB-32874, PDB-7wwv: DNA bound-ICP1 Csy complex 手法: EM (単粒子) / 解像度: 3.2 Å PDB-7wko: ICP1 Csy1-2 complex 手法: X-RAY DIFFRACTION / 解像度: 2.3 Å PDB-7wkp: ICP1 Csy4 手法: X-RAY DIFFRACTION / 解像度: 2 Å
化合物	ChemComp-HOH: WATER
由来	vibrio phage icp1_2011_a (ファージ)
キーワード	RNA BINDING PROTEIN / CRISPR-Cas / phage ICP1 / RNA BINDING PROTEIN/RNA / Complex / RNA BINDING PROTEIN-RNA complex / CRISPR-Cas system / RNA / RNA BINDING PROTEIN/RNA/DNA / RNA BINDING PROTEIN-RNA-DNA complex