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-Structure paper
タイトル | Structural basis of bacteriophage lambda capsid maturation. |
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ジャーナル・号・ページ | Structure, Vol. 30, Issue 4, Page 637-645.e3, Year 2022 |
掲載日 | 2022年4月7日 |
![]() | Chang Wang / Jianwei Zeng / Jiawei Wang / ![]() |
PubMed 要旨 | Bacteriophage lambda is an excellent model system for studying capsid assembly of double-stranded DNA (dsDNA) bacteriophages, some dsDNA archaeal viruses, and herpesviruses. HK97 fold coat proteins ...Bacteriophage lambda is an excellent model system for studying capsid assembly of double-stranded DNA (dsDNA) bacteriophages, some dsDNA archaeal viruses, and herpesviruses. HK97 fold coat proteins initially assemble into a precursor capsid (procapsid) and subsequent genome packaging triggers morphological expansion of the shell. An auxiliary protein is required to stabilize the expanded capsid structure. To investigate the capsid maturation mechanism, we determined the cryo-electron microscopy structures of the bacteriophage lambda procapsid and mature capsid at 3.88 Å and 3.76 Å resolution, respectively. Besides primarily rigid body movements of common features of the major capsid protein gpE, large-scale structural rearrangements of other domains occur simultaneously. Assembly of intercapsomers within the procapsid is facilitated by layer-stacking effects at 3-fold vertices. Upon conformational expansion of the capsid shell, the missing top layer is fulfilled by cementing the gpD protein against the internal pressure of DNA packaging. Our structures illuminate the assembly mechanisms of dsDNA viruses. |
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手法 | EM (単粒子) |
解像度 | 3.76 - 5.6 Å |
構造データ | EMDB-32004, PDB-7vi9: EMDB-32005, PDB-7via: EMDB-32010, PDB-7vii: EMDB-32011, PDB-7vik: |
由来 |
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![]() | VIRUS / bacteriophage lambda; capsid; procapsid; capsid maturation; virus structure; cryo-EM; auxiliary protein; conformational expansion; cementing protein; DNA packaging |