EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures of the nitrogenase complex prepared under catalytic turnover conditions.
ジャーナル・号・ページ	Science, Vol. 377, Issue 6608, Page 865-869, Year 2022
掲載日	2022年8月19日
著者	Hannah L Rutledge / Brian D Cook / Hoang P M Nguyen / Mark A Herzik / F Akif Tezcan /
PubMed 要旨	The enzyme nitrogenase couples adenosine triphosphate (ATP) hydrolysis to the multielectron reduction of atmospheric dinitrogen into ammonia. Despite extensive research, the mechanistic details of ...The enzyme nitrogenase couples adenosine triphosphate (ATP) hydrolysis to the multielectron reduction of atmospheric dinitrogen into ammonia. Despite extensive research, the mechanistic details of ATP-dependent energy transduction and dinitrogen reduction by nitrogenase are not well understood, requiring new strategies to monitor its structural dynamics during catalytic action. Here, we report cryo-electron microscopy structures of the nitrogenase complex prepared under enzymatic turnover conditions. We observe that asymmetry governs all aspects of the nitrogenase mechanism, including ATP hydrolysis, protein-protein interactions, and catalysis. Conformational changes near the catalytic iron-molybdenum cofactor are correlated with the nucleotide-hydrolysis state of the enzyme.
リンク	Science / PubMed:35901182 / PubMed Central
手法	EM (単粒子)
解像度	1.91 - 3.01 Å
構造データ	26756 7ut6 EMDB-26756, PDB-7ut6: C1 symmetric cryoEM structure of Azotobacter vinelandii MoFeP under non-turnover conditions 手法: EM (単粒子) / 解像度: 1.91 Å 26757 7ut7 EMDB-26757, PDB-7ut7: C2 symmetric cryoEM structure of Azotobacter vinelandii MoFeP under non-turnover conditions 手法: EM (単粒子) / 解像度: 1.91 Å EMDB-26758: Consensus cryoEM map of Azotobacter vinelandii MoFeP in a 1:1 complex (ATP-bound) with FeP during catalytic N2 reduction 手法: EM (単粒子) / 解像度: 2.38 Å EMDB-26759: Locally refined cryoEM map of Azotobacter vinelandii FeP in a 1:1 complex (ATP-bound) with MoFeP during catalytic N2 reduction 手法: EM (単粒子) / 解像度: 2.75 Å 26760 7ut8 EMDB-26760, PDB-7ut8: CryoEM structure of Azotobacter vinelandii nitrogenase complex (1:1 FeP:MoFeP, ATP-bound) during catalytic N2 reduction 手法: EM (単粒子) / 解像度: 2.43 Å EMDB-26761: Consensus cryoEM map of Azotobacter vinelandii MoFeP in a 1:1 complex (ADP/ATP-bound) with FeP during catalytic N2 reduction 手法: EM (単粒子) / 解像度: 2.34 Å EMDB-26762: Locally refined cryoEM map of Azotobacter vinelandii FeP in a 1:1 complex (ADP/ATP-bound) with MoFeP during catalytic N2 reduction 手法: EM (単粒子) / 解像度: 3.01 Å 26763 7ut9 EMDB-26763, PDB-7ut9: CryoEM structure of Azotobacter vinelandii nitrogenase complex (1:1 FeP:MoFeP, ADP/ATP-bound) during catalytic N2 reduction 手法: EM (単粒子) / 解像度: 2.44 Å 26764 7uta EMDB-26764, PDB-7uta: CryoEM structure of Azotobacter vinelandii nitrogenase complex (2:1 FeP:MoFeP) inhibited by BeFx during catalytic N2 reduction 手法: EM (単粒子) / 解像度: 2.4 Å 27639 8dpn EMDB-27639, PDB-8dpn: CryoEM structure of Azotobacter vinelandii nitrogenase MoFeP during catalytic N2 reduction 手法: EM (単粒子) / 解像度: 2.49 Å
化合物	ChemComp-HCA: 3-HYDROXY-3-CARBOXY-ADIPIC ACID / (2R)-2-ヒドロキシ-1,2,4-ブタントリカルボン酸 ChemComp-ICS: iron-sulfur-molybdenum cluster with interstitial carbon ChemComp-FE: Unknown entry ChemComp-CLF: FE(8)-S(7) CLUSTER ChemComp-HOH: WATER ChemComp-SF4: IRON/SULFUR CLUSTER ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-0BE: BERYLLIUM
由来	azotobacter vinelandii dj (窒素固定)
キーワード	OXIDOREDUCTASE / nitrogenase / MoFeP / nitrogen fixation / FeP