タイトル | Structural basis for Cas9 off-target activity. |
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ジャーナル・号・ページ | Cell, Vol. 185, Page 4067-4081.e21, Year 2022 |
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掲載日 | 2022年1月10日 (構造データの登録日) |
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著者 | Pacesa, M. / Lin, C.H. / Clery, A. / Saha, A. / Arantes, P.R. / Bargsten, K. / Irby, M.J. / Allain, F.H. / Palermo, G. / Cameron, P. ...Pacesa, M. / Lin, C.H. / Clery, A. / Saha, A. / Arantes, P.R. / Bargsten, K. / Irby, M.J. / Allain, F.H. / Palermo, G. / Cameron, P. / Donohoue, P.D. / Jinek, M. |
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リンク | Cell / PubMed:36306733 |
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手法 | X線回折 |
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解像度 | 2.25 - 3.3 Å |
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構造データ | PDB-7qqo: SpCas9 bound to AAVS1 off-target1 DNA substrate 手法: X-RAY DIFFRACTION / 解像度: 3 Å PDB-7qqp: SpCas9 bound to AAVS1 off-target3 DNA substrate 手法: X-RAY DIFFRACTION / 解像度: 2.6 Å PDB-7qqq: SpCas9 bound to AAVS1 off-target4 DNA substrate 手法: X-RAY DIFFRACTION / 解像度: 2.65 Å PDB-7qqr: SpCas9 bound to AAVS1 off-target5 DNA substrate 手法: X-RAY DIFFRACTION / 解像度: 2.75 Å PDB-7qqs: SpCas9 bound to FANCF on-target DNA substrate 手法: X-RAY DIFFRACTION / 解像度: 2.4 Å PDB-7qqt: SpCas9 bound to FANCF off-target1 DNA substrate 手法: X-RAY DIFFRACTION / 解像度: 2.5 Å PDB-7qqu: SpCas9 bound to FANCF off-target2 DNA substrate 手法: X-RAY DIFFRACTION / 解像度: 2.45 Å PDB-7qqv: SpCas9 bound to FANCF off-target3 DNA substrate 手法: X-RAY DIFFRACTION / 解像度: 2.25 Å PDB-7qqw: SpCas9 bound to FANCF off-target4 DNA substrate 手法: X-RAY DIFFRACTION / 解像度: 3.1 Å PDB-7qqx: SpCas9 bound to FANCF off-target5 DNA substrate 手法: X-RAY DIFFRACTION / 解像度: 2.4 Å PDB-7qqz: SpCas9 bound to FANCF off-target7 DNA substrate 手法: X-RAY DIFFRACTION / 解像度: 2.25 Å PDB-7qr0: SpCas9 bound to TRAC off-target1 DNA substrate 手法: X-RAY DIFFRACTION / 解像度: 2.3 Å PDB-7qr1: SpCas9 bound to TRAC off-target2 DNA substrate 手法: X-RAY DIFFRACTION / 解像度: 2.6 Å PDB-7qr5: SpCas9 bound to FANCF off-target6 DNA substrate 手法: X-RAY DIFFRACTION / 解像度: 3.3 Å PDB-7qr7: SpCas9 bound to AAVS1 off-target2 DNA substrate 手法: X-RAY DIFFRACTION / 解像度: 3 Å PDB-7qr8: SpCas9 bound to PTPRC off-target1 DNA substrate 手法: X-RAY DIFFRACTION / 解像度: 2.75 Å PDB-7zo1: SpCas9 bound to CD34 off-target9 DNA substrate 手法: X-RAY DIFFRACTION / 解像度: 2.4 Å |
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化合物 | |
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由来 | - synthetic construct (人工物)
streptococcus pyogenes (化膿レンサ球菌)
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キーワード | HYDROLASE / crispr / cas9 / off-target / ternary complex / ternary / deletion |
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