EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Histone H1 binding to nucleosome arrays depends on linker DNA length and trajectory.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 29, Issue 5, Page 493-501, Year 2022
掲載日	2022年5月17日
著者	Marco Dombrowski / Maik Engeholm / Christian Dienemann / Svetlana Dodonova / Patrick Cramer /
PubMed 要旨	Throughout the genome, nucleosomes often form regular arrays that differ in nucleosome repeat length (NRL), occupancy of linker histone H1 and transcriptional activity. Here, we report cryo-EM ...Throughout the genome, nucleosomes often form regular arrays that differ in nucleosome repeat length (NRL), occupancy of linker histone H1 and transcriptional activity. Here, we report cryo-EM structures of human H1-containing tetranucleosome arrays with four physiologically relevant NRLs. The structures show a zig-zag arrangement of nucleosomes, with nucleosomes 1 and 3 forming a stack. H1 binding to stacked nucleosomes depends on the NRL, whereas H1 always binds to the non-stacked nucleosomes 2 and 4. Short NRLs lead to altered trajectories of linker DNA, and these altered trajectories sterically impair H1 binding to the stacked nucleosomes in our structures. As the NRL increases, linker DNA trajectories relax, enabling H1 contacts and binding. Our results provide an explanation for why arrays with short NRLs are depleted of H1 and suited for transcription, whereas arrays with long NRLs show full H1 occupancy and can form transcriptionally silent heterochromatin regions.
リンク	Nat Struct Mol Biol / PubMed:35581345 / PubMed Central
手法	EM (単粒子)
解像度	3.8 - 11.0 Å
構造データ	13356 7pet EMDB-13356, PDB-7pet: The 4x177 nucleosome array containing H1 手法: EM (単粒子) / 解像度: 9.5 Å 13357 7peu EMDB-13357, PDB-7peu: Trinucleosome of the 4x177 nucleosome array containing H1 手法: EM (単粒子) / 解像度: 7.2 Å 13358 7pev EMDB-13358, PDB-7pev: Nucleosome stack of the 4x177 nucleosome array containing H1 手法: EM (単粒子) / 解像度: 6.0 Å 13359 7pew EMDB-13359, PDB-7pew: Nucleosome 1 of the 4x177 nucleosome array containing H1 手法: EM (単粒子) / 解像度: 4.6 Å 13360 7pex EMDB-13360, PDB-7pex: Nucleosome 2 of the 4x177 nucleosome array containing H1 手法: EM (単粒子) / 解像度: 5.1 Å 13361 7pey EMDB-13361, PDB-7pey: Nucleosome 3 of the 4x177 nucleosome array containing H1 手法: EM (単粒子) / 解像度: 4.5 Å 13362 7pez EMDB-13362, PDB-7pez: Nucleosome 4 of the 4x177 nucleosome array containing H1 手法: EM (単粒子) / 解像度: 7.9 Å 13363 7pf0 EMDB-13363, PDB-7pf0: Trinucleosome of the 4x177 nucleosome array containing H1 手法: EM (単粒子) / 解像度: 11.0 Å 13365 7pf2 EMDB-13365, PDB-7pf2: Nucleosome stack of the 4x187 nucleosome array containing H1 手法: EM (単粒子) / 解像度: 5.1 Å 13366 7pf3 EMDB-13366, PDB-7pf3: Nucleosome 4 of the 4x187 nucleosome array containing H1 手法: EM (単粒子) / 解像度: 4.0 Å 13367 7pf4 EMDB-13367, PDB-7pf4: Nucleosome 3 of the 4x187 nucleosome array containing H1 手法: EM (単粒子) / 解像度: 4.0 Å 13368 7pf5 EMDB-13368, PDB-7pf5: Nucleosome 2 of the 4x187 nucleosome array containing H1 手法: EM (単粒子) / 解像度: 3.8 Å 13369 7pf6 EMDB-13369, PDB-7pf6: Nucleosome 1 of the 4x187 nucleosome array containing H1 手法: EM (単粒子) / 解像度: 4.0 Å 13370 7pfa EMDB-13370, PDB-7pfa: Trinucleosome of the 4x197 nucleosome array containing H1 手法: EM (単粒子) / 解像度: 9.7 Å 13371 7pfc EMDB-13371, PDB-7pfc: Nucleosome stack of the 4x197 nucleosome array containing H1 手法: EM (単粒子) / 解像度: 6.4 Å 13372 7pfd EMDB-13372, PDB-7pfd: Nucleosome 1 of the 4x197 nucleosome array containing H1 手法: EM (単粒子) / 解像度: 4.4 Å 13373 7pfe EMDB-13373, PDB-7pfe: Nucleosome 2 of the 4x197 nucleosome array containing H1 手法: EM (単粒子) / 解像度: 4.4 Å 13374 7pff EMDB-13374, PDB-7pff: Nucleosome 3 of the 4x197 nucleosome array containing H1 手法: EM (単粒子) / 解像度: 4.3 Å 13379 7pft EMDB-13379, PDB-7pft: Trinucleosome of the 4x207 nucleosome array containing H1 手法: EM (単粒子) / 解像度: 9.8 Å 13380 7pfu EMDB-13380, PDB-7pfu: Nucleosome stack of the 4x207 nucleosome array containing H1 手法: EM (単粒子) / 解像度: 5.0 Å 13381 7pfv EMDB-13381, PDB-7pfv: Nucleosome 1 of the 4x207 nucleosome array containing H1 手法: EM (単粒子) / 解像度: 4.4 Å 13382 7pfw EMDB-13382, PDB-7pfw: Nucleosome 2 of the 4x207 nucleosome array containing H1 手法: EM (単粒子) / 解像度: 5.2 Å 13383 7pfx EMDB-13383, PDB-7pfx: Nucleosome 3 of the 4x207 nucleosome array containing H1 手法: EM (単粒子) / 解像度: 4.3 Å
由来	homo sapiens (ヒト) synthetic construct (人工物)
キーワード	DNA BINDING PROTEIN (DNA結合タンパク質) / Chromatin (クロマチン) / Nucleosomes (ヌクレオソーム) / Linker Histone / Nucleosome (ヌクレオソーム)