+検索条件
-Structure paper
タイトル | Cryo-EM structures of a LptDE transporter in complex with Pro-macrobodies offer insight into lipopolysaccharide translocation. |
---|---|
ジャーナル・号・ページ | Nat Commun, Vol. 13, Issue 1, Page 1826, Year 2022 |
掲載日 | 2022年4月5日 |
![]() | Mathieu Botte / Dongchun Ni / Stephan Schenck / Iwan Zimmermann / Mohamed Chami / Nicolas Bocquet / Pascal Egloff / Denis Bucher / Matilde Trabuco / Robert K Y Cheng / Janine D Brunner / Markus A Seeger / Henning Stahlberg / Michael Hennig / ![]() ![]() |
PubMed 要旨 | Lipopolysaccharides are major constituents of the extracellular leaflet in the bacterial outer membrane and form an effective physical barrier for environmental threats and for antibiotics in Gram- ...Lipopolysaccharides are major constituents of the extracellular leaflet in the bacterial outer membrane and form an effective physical barrier for environmental threats and for antibiotics in Gram-negative bacteria. The last step of LPS insertion via the Lpt pathway is mediated by the LptD/E protein complex. Detailed insights into the architecture of LptDE transporter complexes have been derived from X-ray crystallography. However, no structure of a laterally open LptD transporter, a transient state that occurs during LPS release, is available to date. Here, we report a cryo-EM structure of a partially opened LptDE transporter in complex with rigid chaperones derived from nanobodies, at 3.4 Å resolution. In addition, a subset of particles allows to model a structure of a laterally fully opened LptDE complex. Our work offers insights into the mechanism of LPS insertion, provides a structural framework for the development of antibiotics targeting LptD and describes a highly rigid chaperone scaffold to enable structural biology of challenging protein targets. |
![]() | ![]() ![]() ![]() |
手法 | EM (単粒子) / X線回折 |
解像度 | 2 - 3.4 Å |
構造データ | EMDB-12990: Cryo-EM structure of N. gonorhoeae LptDE in complex with ProMacrobodies ![]() PDB-7omt: |
化合物 | ![]() ChemComp-P6G: ![]() ChemComp-MG: ![]() ChemComp-HOH: |
由来 |
|
![]() | TRANSPORT PROTEIN / Outer membrane protein LPS transporter Bacterial transporter Neisseria gonorrhoeae Drug target ProMacrobodies Structural chaperone Cryo-electron microscopy / IMMUNE SYSTEM / nanobody Cryo-EM Chaperone MBP |