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-Structure paper
タイトル | Structures and implications of TBP-nucleosome complexes. |
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ジャーナル・号・ページ | Proc Natl Acad Sci U S A, Vol. 118, Issue 30, Year 2021 |
掲載日 | 2021年7月27日 |
著者 | Haibo Wang / Le Xiong / Patrick Cramer / |
PubMed 要旨 | The TATA box-binding protein (TBP) is highly conserved throughout eukaryotes and plays a central role in the assembly of the transcription preinitiation complex (PIC) at gene promoters. TBP binds and ...The TATA box-binding protein (TBP) is highly conserved throughout eukaryotes and plays a central role in the assembly of the transcription preinitiation complex (PIC) at gene promoters. TBP binds and bends DNA, and directs adjacent binding of the transcription factors TFIIA and TFIIB for PIC assembly. Here, we show that yeast TBP can bind to a nucleosome containing the Widom-601 sequence and that TBP-nucleosome binding is stabilized by TFIIA. We determine three cryo-electron microscopy (cryo-EM) structures of TBP-nucleosome complexes, two of them containing also TFIIA. TBP can bind to superhelical location (SHL) -6, which contains a TATA-like sequence, but also to SHL +2, which is GC-rich. Whereas binding to SHL -6 can occur in the absence of TFIIA, binding to SHL +2 is only observed in the presence of TFIIA and goes along with detachment of upstream terminal DNA from the histone octamer. TBP-nucleosome complexes are sterically incompatible with PIC assembly, explaining why a promoter nucleosome generally impairs transcription and must be moved before initiation can occur. |
リンク | Proc Natl Acad Sci U S A / PubMed:34301908 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.5 - 3.4 Å |
構造データ | EMDB-12897, PDB-7oh9: EMDB-12898, PDB-7oha: EMDB-12899, PDB-7ohb: EMDB-12900, PDB-7ohc: |
由来 |
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キーワード | TRANSCRIPTION / DNA binding protein nucleosome / NUCLEAR PROTEIN / nucleosome |