EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Mapping inhibitory sites on the RNA polymerase of the 1918 pandemic influenza virus using nanobodies.
ジャーナル・号・ページ	Nat Commun, Vol. 13, Issue 1, Page 251, Year 2022
掲載日	2022年1月11日
著者	Jeremy R Keown / Zihan Zhu / Loïc Carrique / Haitian Fan / Alexander P Walker / Itziar Serna Martin / Els Pardon / Jan Steyaert / Ervin Fodor / Jonathan M Grimes /
PubMed 要旨	Influenza A viruses cause seasonal epidemics and global pandemics, representing a considerable burden to healthcare systems. Central to the replication cycle of influenza viruses is the viral RNA- ...Influenza A viruses cause seasonal epidemics and global pandemics, representing a considerable burden to healthcare systems. Central to the replication cycle of influenza viruses is the viral RNA-dependent RNA polymerase which transcribes and replicates the viral RNA genome. The polymerase undergoes conformational rearrangements and interacts with viral and host proteins to perform these functions. Here we determine the structure of the 1918 influenza virus polymerase in transcriptase and replicase conformations using cryo-electron microscopy (cryo-EM). We then structurally and functionally characterise the binding of single-domain nanobodies to the polymerase of the 1918 pandemic influenza virus. Combining these functional and structural data we identify five sites on the polymerase which are sensitive to inhibition by nanobodies. We propose that the binding of nanobodies at these sites either prevents the polymerase from assuming particular functional conformations or interactions with viral or host factors. The polymerase is highly conserved across the influenza A subtypes, suggesting these sites as effective targets for potential influenza antiviral development.
リンク	Nat Commun / PubMed:35017564 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	1.68 - 6.72 Å
構造データ	12322 7nha EMDB-12322, PDB-7nha: 1918 H1N1 Viral influenza polymerase heterotrimer - Endonuclease and priming loop ordered (Class2a) 手法: EM (単粒子) / 解像度: 2.91 Å 12323 7nhc EMDB-12323, PDB-7nhc: 1918 H1N1 Viral influenza polymerase heterotrimer - Endonuclease ordered (Class2b) 手法: EM (単粒子) / 解像度: 2.87 Å 12342 7nhx EMDB-12342, PDB-7nhx: 1918 H1N1 Viral influenza polymerase heterotrimer - full transcriptase (Class1) 手法: EM (単粒子) / 解像度: 3.23 Å 12348 7ni0 EMDB-12348, PDB-7ni0: 1918 H1N1 Viral influenza polymerase heterotrimer - Replicase (class 3) 手法: EM (単粒子) / 解像度: 3.32 Å 12361 7nik EMDB-12361, PDB-7nik: 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8189 core 手法: EM (単粒子) / 解像度: 6.2 Å 12362 7nil EMDB-12362, PDB-7nil: 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8190 core 手法: EM (単粒子) / 解像度: 5.01 Å 12363 7nir EMDB-12363, PDB-7nir: 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8191 core 手法: EM (単粒子) / 解像度: 6.7 Å 12364 7nis EMDB-12364, PDB-7nis: 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8192 core 手法: EM (単粒子) / 解像度: 5.96 Å 12371 7nj3 EMDB-12371, PDB-7nj3: 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8196 core 手法: EM (単粒子) / 解像度: 4.48 Å 12372 7nj4 EMDB-12372, PDB-7nj4: 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8198 core 手法: EM (単粒子) / 解像度: 5.84 Å 12373 7nj5 EMDB-12373, PDB-7nj5: 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8199 core 手法: EM (単粒子) / 解像度: 4.63 Å 12375 7nj7 EMDB-12375, PDB-7nj7: 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8200 core 手法: EM (単粒子) / 解像度: 4.82 Å 12428 7nk1 EMDB-12428, PDB-7nk1: 1918 Influenza virus polymerase heterotirmer in complex with vRNA promoters and Nb8201 手法: EM (単粒子) / 解像度: 4.22 Å 12429 7nk2 EMDB-12429, PDB-7nk2: 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8202 core 手法: EM (単粒子) / 解像度: 4.84 Å 12430 7nk4 EMDB-12430, PDB-7nk4: 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8203 core 手法: EM (単粒子) / 解像度: 5.32 Å 12431 7nk6 EMDB-12431, PDB-7nk6: 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8204 手法: EM (単粒子) / 解像度: 6.72 Å 12433 7nk8 EMDB-12433, PDB-7nk8: 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8205 core 手法: EM (単粒子) / 解像度: 5.34 Å 12435 7nka EMDB-12435, PDB-7nka: 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8206 手法: EM (単粒子) / 解像度: 4.07 Å 12437 7nkc EMDB-12437, PDB-7nkc: 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8207 手法: EM (単粒子) / 解像度: 4.46 Å 12440 7nki EMDB-12440, PDB-7nki: 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8209 core 手法: EM (単粒子) / 解像度: 4.67 Å 12447 7nkr EMDB-12447, PDB-7nkr: 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8210 手法: EM (単粒子) / 解像度: 5.6 Å PDB-7nfq: Fujian capmidlink domain in complex with Nb8193 手法: X-RAY DIFFRACTION / 解像度: 1.68 Å PDB-7nfr: Fujian capmidlink domain in complex with Nb8194 手法: X-RAY DIFFRACTION / 解像度: 1.88 Å PDB-7nft: Fujian capbinding domain in complex with Nb8208 手法: X-RAY DIFFRACTION / 解像度: 3.14 Å
化合物	ChemComp-GOL: GLYCEROL / グリセロ-ル ChemComp-PEG: DI(HYDROXYETHYL)ETHER / ジエチレングリコ-ル ChemComp-HOH: WATER ChemComp-SO4: SULFATE ION / 硫酸ジアニオン
由来	influenza a virus (strain a/brevig mission/1/1918 h1n1) (A型インフルエンザウイルス) synthetic construct (人工物) staphylococcus aureus (黄色ブドウ球菌) influenza a virus (a/brevig mission/1/1918(h1n1)) (A型インフルエンザウイルス) camelidae mixed library (哺乳類) Influenza A virus (A/Brevig Mission/1/1918(H1N1)) influenza a virus (a/duck/fujian/13/2002(h5n1)) (A型インフルエンザウイルス) homo sapiens (ヒト)
キーワード	VIRAL PROTEIN / Influenza polymerase / cap-binding domain / nanobody / Influenza / RNA polymerase / H1N1 / 1918 / Influenza virus / polymerase / RdRp