EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Architecture of the cytoplasmic face of the nuclear pore.
ジャーナル・号・ページ	Science, Vol. 376, Issue 6598, Page eabm9129, Year 2022
掲載日	2022年6月10日
著者	Christopher J Bley / Si Nie / George W Mobbs / Stefan Petrovic / Anna T Gres / Xiaoyu Liu / Somnath Mukherjee / Sho Harvey / Ferdinand M Huber / Daniel H Lin / Bonnie Brown / Aaron W Tang / Emily J Rundlet / Ana R Correia / Shane Chen / Saroj G Regmi / Taylor A Stevens / Claudia A Jette / Mary Dasso / Alina Patke / Alexander F Palazzo / Anthony A Kossiakoff / André Hoelz /
PubMed 要旨	INTRODUCTION The subcellular compartmentalization of eukaryotic cells requires selective transport of folded proteins and protein-nucleic acid complexes. Embedded in nuclear envelope pores, which are ...INTRODUCTION The subcellular compartmentalization of eukaryotic cells requires selective transport of folded proteins and protein-nucleic acid complexes. Embedded in nuclear envelope pores, which are generated by the circumscribed fusion of the inner and outer nuclear membranes, nuclear pore complexes (NPCs) are the sole bidirectional gateways for nucleocytoplasmic transport. The ~110-MDa human NPC is an ~1000-protein assembly that comprises multiple copies of ~34 different proteins, collectively termed nucleoporins. The symmetric core of the NPC is composed of an inner ring encircling the central transport channel and outer rings formed by Y‑shaped coat nucleoporin complexes (CNCs) anchored atop both sides of the nuclear envelope. The outer rings are decorated with compartment‑specific asymmetric nuclear basket and cytoplasmic filament nucleoporins, which establish transport directionality and provide docking sites for transport factors and the small guanosine triphosphatase Ran. The cytoplasmic filament nucleoporins also play an essential role in the irreversible remodeling of messenger ribonucleoprotein particles (mRNPs) as they exit the central transport channel. Unsurprisingly, the NPC's cytoplasmic face represents a hotspot for disease‑associated mutations and is commonly targeted by viral virulence factors. RATIONALE Previous studies established a near-atomic composite structure of the human NPC's symmetric core by combining (i) biochemical reconstitution to elucidate the interaction network between symmetric nucleoporins, (ii) crystal and single-particle cryo-electron microscopy structure determination of nucleoporins and nucleoporin complexes to reveal their three-dimensional shape and the molecular details of their interactions, (iii) quantitative docking in cryo-electron tomography (cryo-ET) maps of the intact human NPC to uncover nucleoporin stoichiometry and positioning, and (iv) cell‑based assays to validate the physiological relevance of the biochemical and structural findings. In this work, we extended our approach to the cytoplasmic filament nucleoporins to reveal the near-atomic architecture of the cytoplasmic face of the human NPC. RESULTS Using biochemical reconstitution, we elucidated the protein-protein and protein-RNA interaction networks of the human and cytoplasmic filament nucleoporins, establishing an evolutionarily conserved heterohexameric cytoplasmic filament nucleoporin complex (CFNC) held together by a central heterotrimeric coiled‑coil hub that tethers two separate mRNP‑remodeling complexes. Further biochemical analysis and determination of a series of crystal structures revealed that the metazoan‑specific cytoplasmic filament nucleoporin NUP358 is composed of 16 distinct domains, including an N‑terminal S‑shaped α‑helical solenoid followed by a coiled‑coil oligomerization element, numerous Ran‑interacting domains, an E3 ligase domain, and a C‑terminal prolyl‑isomerase domain. Physiologically validated quantitative docking into cryo-ET maps of the intact human NPC revealed that pentameric NUP358 bundles, conjoined by the oligomerization element, are anchored through their N‑terminal domains to the central stalk regions of the CNC, projecting flexibly attached domains as far as ~600 Å into the cytoplasm. Using cell‑based assays, we demonstrated that NUP358 is dispensable for the architectural integrity of the assembled interphase NPC and RNA export but is required for efficient translation. After NUP358 assignment, the remaining 4-shaped cryo‑ET density matched the dimensions of the CFNC coiled‑coil hub, in close proximity to an outer-ring NUP93. Whereas the N-terminal NUP93 assembly sensor motif anchors the properly assembled related coiled‑coil channel nucleoporin heterotrimer to the inner ring, biochemical reconstitution confirmed that the NUP93 assembly sensor is reused in anchoring the CFNC to the cytoplasmic face of the human NPC. By contrast, two CFNCs are anchored by a divergent mechanism that involves assembly sensors located in unstructured portions of two CNC nucleoporins. Whereas unassigned cryo‑ET density occupies the NUP358 and CFNC binding sites on the nuclear face, docking of the nuclear basket component ELYS established that the equivalent position on the cytoplasmic face is unoccupied, suggesting that mechanisms other than steric competition promote asymmetric distribution of nucleoporins. CONCLUSION We have substantially advanced the biochemical and structural characterization of the asymmetric nucleoporins' architecture and attachment at the cytoplasmic and nuclear faces of the NPC. Our near‑atomic composite structure of the human NPC's cytoplasmic face provides a biochemical and structural framework for elucidating the molecular basis of mRNP remodeling, viral virulence factor interference with NPC function, and the underlying mechanisms of nucleoporin diseases at the cytoplasmic face of the NPC. [Figure: see text].
リンク	Science / PubMed:35679405 / PubMed Central
手法	X線回折 / EM (サブトモグラム平均)
解像度	1.1 - 37 Å
構造データ	PDB-7mni: Crystal structure of the N-terminal domain of NUP88 in complex with NUP98 C-terminal Autoproteolytic Domain 手法: X-RAY DIFFRACTION / 解像度: 2 Å PDB-7mnj: Crystal structure of the N-terminal domain of NUP358/RanBP2 (residues 145-673) 手法: X-RAY DIFFRACTION / 解像度: 3.8 Å PDB-7mnk: Crystal structure of the tetramerization element of NUP358/RanBP2 (residues 805-832) 手法: X-RAY DIFFRACTION / 解像度: 1.1 Å PDB-7mnl: Crystal structure of the N-terminal domain of NUP358/RanBP2 (residues 1-752) in complex with Fab fragment 手法: X-RAY DIFFRACTION / 解像度: 3.95 Å PDB-7mnm: Crystal structure of the N-terminal domain of NUP358/RanBP2 (residues 1-752) T585M mutant in complex with Fab fragment 手法: X-RAY DIFFRACTION / 解像度: 4.7 Å PDB-7mnn: Crystal structure of the N-terminal domain of NUP358/RanBP2 (residues 1-752) T653I mutant in complex with Fab fragment 手法: X-RAY DIFFRACTION / 解像度: 6.7 Å PDB-7mno: Crystal structure of the N-terminal domain of NUP358/RanBP2 (residues 1-752) I656V mutant in complex with Fab fragment 手法: X-RAY DIFFRACTION / 解像度: 6.73 Å PDB-7mnp: Crystal Structure of the ZnF2 of Nucleoporin NUP358/RanBP2 in complex with Ran-GDP 手法: X-RAY DIFFRACTION / 解像度: 2.05 Å PDB-7mnq: Crystal Structure of the ZnF2 of Nucleoporin NUP358/RanBP2 in complex with Ran-GDP 手法: X-RAY DIFFRACTION / 解像度: 2.05 Å PDB-7mnr: Crystal Structure of the ZnF3 of Nucleoporin NUP358/RanBP2 in complex with Ran-GDP 手法: X-RAY DIFFRACTION / 解像度: 1.8 Å PDB-7mns: Crystal Structure of the ZnF4 of Nucleoporin NUP358/RanBP2 in complex with Ran-GDP 手法: X-RAY DIFFRACTION / 解像度: 2.1 Å PDB-7mnt: Crystal Structure of the ZnF5 or ZnF6 of Nucleoporin NUP358/RanBP2 in complex with Ran-GDP 手法: X-RAY DIFFRACTION / 解像度: 2.45 Å PDB-7mnu: Crystal Structure of the ZnF7 of Nucleoporin NUP358/RanBP2 in complex with Ran-GDP 手法: X-RAY DIFFRACTION / 解像度: 2 Å PDB-7mnv: Crystal Structure of the ZnF8 of Nucleoporin NUP358/RanBP2 in complex with Ran-GDP 手法: X-RAY DIFFRACTION / 解像度: 1.8 Å PDB-7mnw: Crystal Structure of Nup358/RanBP2 Ran-binding domain 1 in complex with Ran-GPPNHP 手法: X-RAY DIFFRACTION / 解像度: 2.4 Å PDB-7mnx: Crystal Structure of Nup358/RanBP2 Ran-binding domain 2 in complex with Ran-GPPNHP 手法: X-RAY DIFFRACTION / 解像度: 2.4 Å PDB-7mny: Crystal Structure of Nup358/RanBP2 Ran-binding domain 3 in complex with Ran-GPPNHP 手法: X-RAY DIFFRACTION / 解像度: 2.7 Å PDB-7mnz: Crystal Structure of Nup358/RanBP2 Ran-binding domain 4 in complex with Ran-GPPNHP 手法: X-RAY DIFFRACTION / 解像度: 2.35 Å PDB-7mo0: Crystal Structure of Nucleoporin NUP50 Ran-Binding Domain in Complex with Ran-GPPNHP 手法: X-RAY DIFFRACTION / 解像度: 2.45 Å PDB-7mo1: Crystal Structure of the ZnF1 of Nucleoporin NUP153 in complex with Ran-GDP 手法: X-RAY DIFFRACTION / 解像度: 1.6 Å PDB-7mo2: Crystal Structure of the ZnF2 of Nucleoporin NUP153 in complex with Ran-GDP 手法: X-RAY DIFFRACTION / 解像度: 1.65 Å PDB-7mo3: Crystal Structure of the ZnF3 of Nucleoporin NUP153 in complex with Ran-GDP, resolution 2.05 Angstrom 手法: X-RAY DIFFRACTION / 解像度: 2.05 Å PDB-7mo4: Crystal Structure of the ZnF3 of Nucleoporin NUP153 in complex with Ran-GDP, resolution 2.4 Angstrom 手法: X-RAY DIFFRACTION / 解像度: 2.4 Å PDB-7mo5: Crystal Structure of the ZnF4 of Nucleoporin NUP153 in complex with Ran-GDP 手法: X-RAY DIFFRACTION / 解像度: 1.55 Å PDB-7tbl: Composite structure of the human nuclear pore complex (NPC) cytoplasmic face generated with a 12A cryo-ET map of the purified HeLa cell NPC 手法: ELECTRON MICROSCOPY / 解像度: 23 Å PDB-7tbm: Composite structure of the dilated human nuclear pore complex (NPC) generated with a 37A in situ cryo-ET map of CD4+ T cell NPC 手法: ELECTRON MICROSCOPY / 解像度: 37 Å
化合物	ChemComp-HOH: WATER / 水 ChemComp-EDO: 1,2-ETHANEDIOL / エチレングリコ-ル / エチレングリコール ChemComp-SO4: SULFATE ION / 硫酸ジアニオン / 硫酸塩 ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子YM / グアノシン二リン酸 ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry ChemComp-GOL: GLYCEROL / グリセロ-ル / グリセリン ChemComp-GNP: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / Gpp(NH)p / GppNHp, GMPPNP, エネルギー貯蔵分子類似体YM / 5'-Guanylyl imidodiphosphate
由来	homo sapiens (ヒト) rattus norvegicus (ドブネズミ)
キーワード	TRANSPORT PROTEIN (運搬体タンパク質) / nuclear pore complex component (核膜孔) / nucleocytoplasmic transport / TRANSFERASE (転移酵素) / TRANSFERASE/Immune System / TRANSFERASE-Immune System complex / TRANSPORT PROTEIN/Immune System / TRANSPORT PROTEIN-Immune System complex / ZINC FINGER (ジンクフィンガー) / complex (small GTPase-nuclear protein) / nuclear pore complex (核膜孔) / alpha-helical solenoid / nuclear pore (核膜孔)